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International Ophthalmology
Published in: International Ophthalmology 9/2020

Open Access 01-09-2020 | Toxoplasmosis | Original Paper

Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

Authors: Jasem Saki, Elham Eskandari, Mostafa Feghhi

Published in: International Ophthalmology | Issue 9/2020

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Abstract

Introduction

Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debilitating vision loss.In Khuzestan province, southwest of Iran, T. gondii infection has been reported to be significant but toxocariasis was rare. However, the frequency of ocular toxoplasmosis and toxocariasis has not been studied in this area. The aim of this study was to evaluate the ocular toxoplasmosis and ocular toxocariasis using serological and molecular methods.

Method

In this case control study, 310 patients were identified by ophthalmologist as ocular toxoplasmosis and then 5 cc of venous blood samples were taken from each of them. Serum samples and buffy coat were prepared and ELISA was used to detect IgG and IgM anti-Toxoplasma antibodies and the molecular PCR was used to detect Toxoplasma DNA parasite in buffy coats. ELISA test was used to detect of IgG anti-Toxocara antibodies.

Results

Totally, for ocular toxoplasmosis, 130 (41.93%) of 310 patients were positive by ELISA, of them 121 (39%) IgG positive and nine (2.9%) IgM positive were diagnosed. Of 121 cases with IgG+, 119 (98.35%) were diagnosed with high IgG avidity indicating chronic phase of the infection. For ocular toxocariasis evaluation, antibodies against Toxocara were not detected in any of the samples. By PCR molecular method, 11 out of 310 patients (3.54%) had T. gondii DNA in the blood. In control, in total, 21 cases were detected positive by serology method, which showed a significant difference with the results of the case group(P < 0.05).By PCR method, only three cases showed positive which also indicated significant difference with result of case group (3 vs 9) (P < 0.05). In the control group, also no anti-toxocara antibodies were found.

Conclusion

It can be concluded that T. gondii in Khuzestan province as the etiologic agent of ocular toxoplasmosis and physicians should consider diagnostic methods for identifying the infection when they visit the patients.
Literature
1.
Chen XG (2009) Toxoplasma gondii: past, present and future. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 5:426–431
2.
Bou G, Figueroa MS, Martí-Belda P, Navas E, Guerrero A (1999) Value of PCR for Detection of Toxoplasma gondii in aqueous humor and blood samples from immunocompetent patients with ocular toxoplasmosis. J Clin Microbiol 11:3465–3468CrossRef
3.
Holland GN, O'Connor GR, Belfort R Jr, Remington JS (1996) Toxoplasmosis. In: Pepose JS, Holland GN, Wilhelmus KR (eds) Ocular infection and immunity, vol 1, 1st edn. St, Mosby Yearbook, Louis, pp 183–223
4.
Khademvatan S, Saki J, Khajeddin N, Izadi-Mazidi M, Beladi R, Shafiee B, Salehi Z (2014) Toxoplasma gondii exposure and the risk of schizophrenia. Jundishapur J Microbiol 7(11):e12776PubMedPubMedCentralCrossRef
5.
Montoya JG, Remington JS (1996) Toxoplasmic chorioretinitis in the setting of acute acquired toxoplasmosis. Clin Infect Dis 23(2):277–282PubMedCrossRef
6.
7.
Masur H, Jones TC, Lempert JA, Cherubini TD (1978) Outbreak of toxoplasmosis in a family and documentation of acquired retinochoroiditis. Am J Med 64(3):396–402PubMedCrossRef
8.
9.
Kayhoe DE, Jacobs L, Beye HK, McCullough NB (1957) Acquired toxoplasmosis: observations on two parasitologically proved cases treated with pyrimethamine and triple sulfonamides. N Engl J Med 257(26):1247–1254PubMedCrossRef
10.
Elmore SA, Jones JL, Conrad PA, Patton S, Lindsay DS, Dubey J (2010) Toxoplasma gondii: epidemiology, feline clinical aspects, and prevention. Trends Parasitol 26(4):190–196PubMedCrossRef
11.
Robinson P (1947) A case of toxoplamosis; with recovery. Ann Paediatr 168(3):134–137PubMed
12.
Luft B, Conley F, Remington J, Laverdiere M, Levine J, Strandberg D, Wagner K, Craven P, File T, Rice N (1983) Outbreak of central-nervous-system toxoplasmosis in western Europe and North America. The Lancet 321(8328):781–784CrossRef
13.
Khademvatan S, Riahi F, Izadi-Mazidi M, Khajeddin N, Yousefi E (2018) Toxoplasma gondii exposure and the risk of attention deficit hyperactivity disorder in children and adolescents. Pediatr Infect Dis J 37(11):1097–1100PubMedCrossRef
14.
15.
Gharavi M, Jalali S, Khademvatan S, Heydari S (2011) Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: comparison of pre-and post-transplantation status. Ann Trop Med Parasitol 105(5):367–371PubMedPubMedCentralCrossRef
16.
Fuccillo D, Madden D, Tzan N, Sever J (1987) Difficulties associated with serological diagnosis of Toxoplasma gondii infections. Diagn Clin Immunol 5(1):8–13PubMed
17.
Horváth KN, Szénási Z, Danka J, Kucsera I (2005) Value of the IgG avidity in the diagnosis of recent toxoplasmosis: a comparative study of four commercially available anti-Toxoplasma gondii IgG avidity assays. Acta Parasitol 50(3):255–260
18.
Jenum P, Holberg-Petersen M, Melby K, Stray-Pedersen B (1998) Diagnosis of congenital Toxoplasma gondii infection by polymerase chain reaction (PCR) on amniotic fluid samples: the Norwegian experience. Apmis 106(7–12):680–686PubMedCrossRef
19.
Cingolani A, De Luca A, Ammassari A, Murri R, Linzalone A, Grillo R, Antinori A (1996) PCR detection of Toxoplasma gondii DNA in CSF for the differential diagnosis of AIDS-related focal brain lesions. J Med Microbiol 45(6):472–476PubMedCrossRef
20.
Kompalic-Cristo A, Frotta C, Suárez-Mutis M, Fernandes O, Britto C (2007) Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res 101(3):619–625PubMedCrossRef
21.
Miyazaki III I (1991) Section III. Nematode zoonoses. 33. Gnathostomiasis. An illustrated book of helminthic zoonoses. International Medical Foundation of Japan, Tokyo, Japan, 368409
22.
Stewart JM, Cubillan LD, Emmett T, Cunningham J (2005) Prevalence, clinical features, and causes of vision loss among patients with ocular toxocariasis. Retina 25(8):1005–1013PubMedCrossRef
23.
Chuah C, Lim M, Seah L, Ling Y, Chee S (2006) Pseudoretinoblastoma in enucleated eyes of Asian patients. Singapore Med J 47(7):617PubMed
24.
Burg JL, Grover CM, Pouletty P, Boothroyd J (1989) Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 27(8):1787–1792PubMedPubMedCentralCrossRef
25.
Mousavi M, Saravani R, Modrek MJ, Shahrakipour M, Sekandarpour S (2016) Detection of Toxoplasma gondii in diabetic patients using the nested PCR assay via RE and B1 genes. Jundishapur J Microbiol 9(2):e29493PubMedPubMedCentralCrossRef
26.
Kwon N-H, Oh M-J, Lee S-P, Lee B-J, Choi D-C (2006) The prevalence and diagnostic value of toxocariasis in unknown eosinophilia. Ann Hematol 85(4):233–238PubMedCrossRef
27.
28.
Bou G, Figueroa MS, Martí-Belda P, Navas E, Guerrero A (1999) Value of PCR for detection of Toxoplasma gondii in aqueous humor and blood samples from immunocompetent patients with ocular toxoplasmosis. J Clin Microbiol 37(11):3465–3468PubMedPubMedCentralCrossRef
29.
Dubey J (2009) History of the discovery of the life cycle of Toxoplasma gondii. Int J Parasitol 39(8):877–882PubMedCrossRef
30.
Glasner PD, Silveira C, Kruszon-Moran D, Martins MC, Burnier M Jr, Silveira S, Camargo ME, Nussenblatt RB, Kaslow RA, Belfort R Jr (1992) An unusually high prevalence of ocular toxoplasmosis in southern Brazil. Am J Ophthalmol 114(2):136–144PubMedCrossRef
31.
Suresh S, Nor-Masniwati S, Nor-Idahriani MN, Wan-Hazabbah W-H, Zeehaida M, Zunaina E (2012) Serological IgG avidity test for ocular toxoplasmosis. Clinical Ophthalmology (Auckland, NZ) 6:147
32.
Rahimi-Esboei B, Zarei M, Mohebali M, Valian HK, Shojaee S, Mahmoudzadeh R, Salabati M (2018) Serologic tests of IgG and IgM antibodies and IgG avidity for diagnosis of ocular toxoplasmosis. Korean J Parasitol 56(2):147PubMedPubMedCentralCrossRef
33.
Jones JL, Kruszon-Moran D, Sanders-Lewis K, Wilson M (2007) Toxoplasma gondii infection in the United States, 1999–2004, decline from the prior decade. Am J Trop Med Hygiene 77(3):405–410CrossRef
34.
35.
Bourdin C, Busse A, Kouamou E, Touafek F, Bodaghi B, Le Hoang P, Mazier D, Paris L, Fekkar A (2014) PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis. J Clin Microbiol 52(11):3987–3991PubMedPubMedCentralCrossRef
Metadata
Title
Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
Authors
Jasem Saki
Elham Eskandari
Mostafa Feghhi
Publication date
01-09-2020
Publisher
Springer Netherlands
Keyword
Toxoplasmosis
Published in
International Ophthalmology / Issue 9/2020
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-020-01393-6

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