Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
International Ophthalmology
Published in: International Ophthalmology 2/2018

01-04-2018 | Original Paper

Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

Authors: Chien-Neng Kuo, Ching-Hsein Chen, San-Ni Chen, Jou-Cheng Huang, Li-Ju Lai, Chien-Hsiung Lai, Chia-Hui Hung, Chien-Hsing Lee, Chung-Yi Chen

Published in: International Ophthalmology | Issue 2/2018

Login to get access

Abstract

Aim

This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.

Methods

CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.

Results

Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.

Conclusions

Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.
Literature
1.
Chang JH, Gabison EE, Kato T, Azar DT (2001) Corneal neovascularization. Curr Opin Ophthalmol 12(4):242–249CrossRefPubMed
2.
Gonzalez L, Loza RJ, Han KY, Sunoqrot S, Cunningham C, Purta P, Drake J, Jain S, Hong S, Chang JH (2013) Nanotechnology in corneal neovascularization therapy—a review. J Ocul Pharmacol Ther 29(2):124–134CrossRefPubMedPubMedCentral
3.
Anand P, Thomas SG, Kunnumakkara AB, Sundaram C, Harikumar KB, Sung B, Tharakan ST, Misra K, Priyadarsini IK, Rajasekharan KN, Aggarwal BB (2008) Biological activities of curcumin and its analogues (Congeners) made by man and mother nature. Biochem Pharmacol 76(11):1590–1611CrossRefPubMed
4.
Dempe JS, Scheerle RK, Pfeiffer E, Metzler M (2013) Metabolism and permeability of curcumin in cultured Caco-2 cells. Mol Nutr Food Res 57(9):1543–1549CrossRefPubMed
5.
Deters M, Knochenwefel H, Lindhorst D, Koal T, Meyer HH, Hansel W, Resch K, Kaever V (2008) Different curcuminoids inhibit T-lymphocyte proliferation independently of their radical scavenging activities. Pharm Res 25(8):1822–1827CrossRefPubMed
6.
Somparn P, Phisalaphong C, Nakornchai S, Unchern S, Morales NP (2007) Comparative antioxidant activities of curcumin and its demethoxy and hydrogenated derivatives. Biol Pharm Bull 30(1):74–78CrossRefPubMed
7.
Srimuangwong K, Tocharus C, Yoysungnoen Chintana P, Suksamrarn A, Tocharus J (2012) Hexahydrocurcumin enhances inhibitory effect of 5-fluorouracil on HT-29 human colon cancer cells. World J Gastroenterol 18(19):2383–2389CrossRefPubMedPubMedCentral
8.
Moohammadaree A, Changtam C, Wicha P, Suksamrarn A, Tocharus J, Tocharus C (2015) Mechanisms of vasorelaxation induced by hexahydrocurcuminin isolated rat thoracic aorta. Phytother Res 29(11):1806–1813CrossRefPubMed
9.
Kuo CN, Yang LC, Yang CT, Chen MF, Lai CH, Chen YH, Chen CH, Chen CH, Wu PC, Kou HK, Tsai JC, Hung CH (2008) A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Curr Eye Res 33(10):839–848CrossRefPubMed
10.
Kuo CN, Yang LC, Yang CT, Lai CH, Chen MF, Chen CY, Chen CH, Wu PC, Kou HK, Chen YJ, Hung CH, Tsai CB (2009) Inhibition of corneal neovascularization with plasmid pigment epithelium-derived factor (p-PEDF) delivered by synthetic amphiphile INTeraction-18 (SAINT-18) vector in an experimental model of rat corneal angiogenesis. Exp Eye Res 89(5):678–685CrossRefPubMed
11.
Lin RJ, Chen CY, Chung LY, Yen CM (2010) Larvicidal activities of ginger (Zingiber officinale) against Angiostrongylus cantonensis. Acta Trop 115(1–2):69–76CrossRefPubMed
12.
Kuo CN, Chen CY, Chen SN, Yang LC, Lai LJ, Lai CH, Chen MF, Hung CH, Chen CH (2013) Inhibition of corneal neovascularization with the combination of bevacizumab and plasmid pigment epithelium-derived factor-synthetic amphiphile INTeraction-18 (p-PEDF-SAINT-18) vector in a rat corneal experimental angiogenesis model. Int J Mol Sci 14(4):8291–8305CrossRefPubMedPubMedCentral
13.
14.
Ding X, Patel M, Chan CC (2009) Molecular pathology of age-related macular degeneration. Prog Retin Eye Res 28(1):1–18CrossRefPubMed
15.
Kubicka-Trzaska A, Karska-Basta I, Romanowska-Dixon B (2014) Current concepts in pathogenesis of age-related macular degeneration. Klin Oczna 116(4):277–282PubMed
16.
Rezaei KA, Toma HS, Cai J, Penn JS, Sternberg P, Kim SJ (2011) Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice. Investig Ophthalmol Vis Sci 52(2):701–707. doi:10.​1167/​iovs.​10-6319 CrossRef
17.
Kenyon BM, Voest EE, Chen CC, Flynn E, Folkman J, D’Amato RJ (1996) A model of angiogenesis in the mouse cornea. Investig Ophthalmol Vis Sci 37(8):1625–1632
18.
Adamis AP, Meklir B, Joyce NC (1991) In situ injury-induced release of basic-fibroblast growth factor from corneal epithelial cells. Am J Pathol 139(5):961–967PubMedPubMedCentral
19.
Klein-Soyer C, Beretz A, Cazenave JP, Driot F, Maffrand JP (1990) Behavior of confluent endothelial cells after irradiation. Modulation of wound repair by heparin and acidic fibroblast growth factor. Biol Cell 68(3):231–238CrossRefPubMed
20.
Benelli U, Bocci G, Danesi R, Lepri A, Bernardini N, Bianchi F, Lupetti M, Dolfi A, Campagni A, Agen C, Nardi M, Del Tacca M (1998) The heparan sulfate suleparoide inhibits rat corneal angiogenesis and in vitro neovascularization. Exp Eye Res 67(2):133–142CrossRefPubMed
21.
Amano S, Rohan R, Kuroki M, Tolentino M, Adamis AP (1998) Requirement for vascular endothelial growth factor in wound- and inflammation-related corneal neovascularization. Investig Ophthalmol Vis Sci 39(1):18–22
22.
23.
Duenas Z, Torner L, Corbacho AM, Ochoa A, Gutierrez-Ospina G, Lopez-Barrera F, Barrios FA, Berger P, Martinez de la Escalera G, Clapp C (1999) Inhibition of rat corneal angiogenesis by 16-kDa prolactin and by endogenous prolactin-like molecules. Investig Ophthalmol Vis Sci 40(11):2498–2505
24.
Ogata N, Wada M, Otsuji T, Jo N, Tombran-Tink J, Matsumura M (2002) Expression of pigment epithelium-derived factor in normal adult rat eye and experimental choroidal neovascularization. Investig Ophthalmol Vis Sci 43(4):1168–1175
Metadata
Title
Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization
Authors
Chien-Neng Kuo
Ching-Hsein Chen
San-Ni Chen
Jou-Cheng Huang
Li-Ju Lai
Chien-Hsiung Lai
Chia-Hui Hung
Chien-Hsing Lee
Chung-Yi Chen
Publication date
01-04-2018
Publisher
Springer Netherlands
Published in
International Ophthalmology / Issue 2/2018
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-017-0530-6

Other articles of this Issue 2/2018

International Ophthalmology 2/2018 Go to the issue

Case Report

Excessive serous retinal detachment during the use of isotretinoin

Original Paper

Impact of dehydration and fasting on intraocular pressure and corneal biomechanics measured by the Ocular Response Analyzer

Original Paper

Bilateral optic disc pathologies as an accompanying feature of comitant strabismus in children

Original Paper

Agreement among Goldmann applanation tonometer, iCare, and Icare PRO rebound tonometers; non-contact tonometer; and Tonopen XL in healthy elderly subjects

Original Paper

Usability and reproducibility of tear meniscus values generated via swept-source optical coherence tomography and the slit lamp with a graticule method

Original Paper

Prevention of myopia by partial correction of hyperopia: a twins study