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International Ophthalmology
Published in: International Ophthalmology 1/2017

01-02-2017 | Original Paper

Confocal microscopy evaluation of stromal fluorescence intensity after standard and accelerated iontophoresis-assisted corneal cross-linking

Authors: Manuela Lanzini, Claudia Curcio, Eberhard Spoerl, Roberta Calienno, Alessandra Mastropasqua, Martina Colasante, Rodolfo Mastropasqua, Mario Nubile, Leonardo Mastropasqua

Published in: International Ophthalmology | Issue 1/2017

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Abstract

The aim of this study is to determine modifications in stromal fluorescence intensity after different corneal cross-linking (CXL) procedures and to correlate stromal fluorescence to corneal biomechanical resistance. For confocal microscopy study, 15 human cadaver corneas were examined. Three served as control (group 1), three were just soaked with iontophoresis procedure (group 2), three were treated with standard epi-off technique (group 3), and six underwent iontophoresis imbibition. Three of later six were irradiated for 30 min with 3 mW/cm2 UVA (group 4) and three for 9 min at 10 mW/cm2 UVA (group 5). Confocal microscopy was performed to quantify the fluorescence intensity in the cornea at different stromal depths. For biomechanical study, 30 human cadaver corneas were randomly divided into five groups and treated as previously described. Static stress–strain measurements of the corneas were performed. Iontophoresis imbibition followed by 10mW/cm2 irradiation proved to increase stromal fluorescence into the corneal stroma and significant differences were revealed between group 3 and 5 both at 100 (p = 0.0171) and 250 µm (p = 0.0024), respectively. Biomechanical analysis showed an improvement of corneal resistance in group 5. Iontophoresis imbibition followed by accelerated irradiation increased the stromal fluorescence and is related to an improvement of biomechanical resistance. This approach may represent a new strategy to achieve greater concentrations of riboflavin without removing corneal epithelium and improve clinical results while reducing the side effects of CXL.
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Metadata
Title
Confocal microscopy evaluation of stromal fluorescence intensity after standard and accelerated iontophoresis-assisted corneal cross-linking
Authors
Manuela Lanzini
Claudia Curcio
Eberhard Spoerl
Roberta Calienno
Alessandra Mastropasqua
Martina Colasante
Rodolfo Mastropasqua
Mario Nubile
Leonardo Mastropasqua
Publication date
01-02-2017
Publisher
Springer Netherlands
Published in
International Ophthalmology / Issue 1/2017
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-016-0266-8

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