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Inflammation
Published in: Inflammation 2/2020

01-04-2020 | Original Article

Differential Immunomodulatory Effect of Carbon Dots Influenced by the Type of Surface Passivation Agent

Authors: Furkan Ayaz, Melis Ozge Alas, Rukan Genc

Published in: Inflammation | Issue 2/2020

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Abstract

Carbon nanodots (CDs) are often synthesized from natural sources including honey, molasses, fruits, and foods, and plant extracts simply through caramelization. They have wide biological applications especially as drug delivery vehicles and bioimaging agent due to their small size and biocompatibility. This article details the synthesis of carbon dots from carob and its derivatives by surface passivation with polyethylene glycol (PEG), polyvinyl alcohol (PVA), and alginate (ALG). We investigated the immune response against CDs and evaluated the effect of surface passivation agents on their immunomodulatory functions. CDPVA had strong anti-inflammatory activities, whereas CDALG were pro-inflammatory. CDPEG had mild anti-inflammatory activities suggesting that these CDs can be used in the drug delivery studies as inert carriers. These results showed that depending on the type of activated groups dominated on the surface, CDs exerted differential effects on the inflammatory potential of the macrophages by changing the pro-inflammatory TNFα and IL6 production levels.
Literature
1.
Iwalewa, E., L. McGaw, V. Naidoo, and J. Eloff. 2007. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. Afr J Biotechnol 6: 2868–2885.
2.
3.
4.
5.
6.
Hancock, R.E.W., A. Nijnik, and D.J. Philpott. 2012. Modulating immunity as a therapy for bacterial infections. Nat Rev Microbiol 10: 243–254.PubMed
7.
8.
Julier, Z., A.J. Park, P.S. Briquez, and M.M. Martino. 2017. Promoting tissue regeneration by modulating the immune system. Acta Biomater 53: 13–28.PubMed
9.
Khalil, D.N., E.L. Smith, R.J. Brentjens, and J.D. Wolchok. 2016. The future of cancer treatment: immunomodulation, CARs and combination immunotherapy. Nat Rev Clin Oncol 13: 273–290.PubMedPubMedCentral
10.
Tan, T.-T., and L.M. Coussens. 2007. Humoral immunity, inflammation and cancer. Curr Opin Immunol 19: 209–216.PubMed
11.
Chen, D.S., and I. Mellman. 2013. Oncology meets immunology: the cancer-immunity cycle. Immunity. 39: 1–10.PubMed
12.
Guevara-Patino, J.A., M.J. Turk, J.D. Wolchok, and A.N. Houghton. 2003. Immunity to cancer through immune recognition of altered self: studies with melanoma. Adv Cancer Res 90: 157–177.PubMed
13.
Valdes-Ramos, R., and A.D. Benitez-Arciniega. 2007. Nutrition and immunity in cancer. Br J Nutr 98 (Suppl 1): S127–S132.PubMed
14.
S.K. Singh, P.P. Kulkarni, and D. Dash. 2013. Biomedical applications of carbon based nanomaterials. Bio Nanotechnol A Revolut Food, Biomed Heal Sci, eds. Debasis Bagchi, Manashi Bagchi, Hiroyoshi Moriyama, Fereidoon Shahidi, 25:443–463. Wiley Online Library.
15.
S.C. Ray, N.R. JANA, Carbon Nanomaterials for biological and medical applications, 2017.
16.
Cha, C., S.R. Shin, N. Annabi, M.R. Dokmeci, and A. Khademhosseini. 2013. Carbon-based nanomaterials: multifunctional materials for biomedical engineering. ACS Nano 7 (4): 2891–2897.PubMedPubMedCentral
17.
Shu, Y., J. Lu, Q. Mao, R. Song, X. Wang, X. Chen, and J. Wang. 2017. Ionic liquid mediated organophilic carbon dots for drug delivery and bioimaging. Carbon N Y 114: 324–333.
18.
Mehta, V.N., S.S. Chettiar, J.R. Bhamore, S.K. Kailasa, and R.M. Patel. 2017. Green synthetic approach for synthesis of fluorescent carbon dots for lisinopril drug delivery system and their confirmations in the cells. J Fluoresc 27: 111–124.PubMed
19.
Yuan, A.Y., B. Guo, L. Hao, and N. Liu. 2017. Doxorubicin-loaded environmentally friendly carbon dots as a novel drug delivery system for nucleus targeted cancer therapy. Colloids Surf B: Biointerfaces 159: 349–359.PubMed
20.
Dehghani, A., S.M. Ardekani, M. Hassan, and V.G. Gomes. 2018. Collagen derived carbon quantum dots for cell imaging in 3D scaffolds via two-photon spectroscopy. Carbon N Y 131: 238–245.
21.
Ayaz, F., M. Özge, A. Melike, and O. Rükan. 2019. Aluminum doped carbon nanodots as potent adjuvants on the mammalian macrophages. Mol Biol Rep: 1–11.
22.
Luo, L., C. Liu, T. He, L. Zeng, J. Xing, Y. Xia, Y. Pan, C. Gong, and A. Wu. 2018. Engineered fluorescent carbon dots as promising immune adjuvants to efficiently enhance cancer immunotherapy. Nanoscale. 10: 22035–22043.PubMed
23.
Liu, C., P. Zhang, X. Zhai, F. Tian, W. Li, J. Yang, Y. Liu, H. Wang, W. Wang, and W. Liu. 2012. Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced fluorescence. Biomaterials. 33: 3604–3613.PubMed
24.
Ke, Y., B. Garg, and Y. Ling. 2014. Waste chicken eggshell as low-cost precursor for efficient synthesis of nitrogen-doped fluorescent carbon nanodots and their multi-functional applications. RSC Adv 4: 58329–58336.
25.
Pal, T., S. Mohiyuddin, and G. Packirisamy. 2018. Facile and green synthesis of multicolor fluorescence carbon dots from curcumin: in vitro and in vivo bioimaging and other applications. ACS Omega 3: 831–843.PubMedPubMedCentral
26.
Park, S.Y., H.U. Lee, E.S. Park, S.C. Lee, J.-W. Lee, S.W. Jeong, C.H. Kim, Y.-C. Lee, Y.S. Huh, and J. Lee. 2014. Photoluminescent green carbon Nanodots from food waste-derived sources: Large-scale synthesis, properties and bio-medical applications. ACS Appl Mater Interfaces 6 (5): 3365–3370.PubMed
27.
Wang, C., D. Sun, K. Zhuo, H. Zhang, and J. Wang. 2015. Simple and green synthesis of nitrogen-, sulfur-, and phosphorus-co- doped carbon dots with tunable luminescence properties and sensing application Chunfeng. RSC Adv 4 (96): 54060–54065.
28.
Luo, X., W. Zhang, Y. Han, X. Chen, L. Zhu, W. Tang, and J. Wang. 2018. N , S co-doped carbon dots based fl uorescent “ on-off-on ” sensor for determination of ascorbic acid in common fruits. Food Chem 258: 214–221.PubMed
29.
Zhang, Y., Y.H. He, P.P. Cui, X.T. Feng, L. Chen, Y.Z. Yang, and X.G. Liu. 2015. Water-soluble, nitrogen-doped fluorescent carbon dots for highly sensitive and selective detection of Hg2+ in aqueous solution. RSC Adv 5 (50): 40393–40401.
30.
Zhao, C., Y. Jiao, Z. Gao, Y. Yang, and H. Li. 2018. N, S co-doped carbon dots for temperature probe and the detection of tetracycline based on the inner filter effect. J Photochem Photobiol A Chem 367: 137–144.
31.
Çalhan, S.D., M.Ö. Alaş, M. Aşık, F.N.D. Kaya, and R. Genç. 2018. One-pot synthesis of hydrophilic and hydrophobic fluorescent carbon dots using deep eutectic solvents as designer reaction media. J Mater Sci 53: 15362–15375.
32.
Alas, M.O., and R. Genc. 2017. An investigation into the role of macromolecules of different polarity as passivating agent on the physical , chemical and structural properties of fluorescent carbon nanodots. J Nanopart Res 19: 185.
33.
Yang, Q., and S.K. Lai. 2015. Anti-PEG immunity: emergence, characteristics, and unaddressed questions. Wiley Interdiscip Rev Nanomedicine Nanobiotechnology 7: 655–677.PubMed
34.
Wan, X., J. Zhang, W. Yu, L. Shen, S. Ji, and T. Hu. 2017. Effect of protein immunogenicity and PEG size and branching on the anti-PEG immune response to PEGylated proteins. Process Biochem 52: 183–191.
35.
Giacalone, G., N. Tsapis, L. Mousnier, H. Chacun, and E. Fattal. 2018. PLA-PEG nanoparticles improve the anti-inflammatory effect of rosiglitazone on macrophages by enhancing drug uptake compared to free rosiglitazone. Materials (Basel) 11: 1845.
36.
Kzhyshkowska, J., A. Gudima, V. Riabov, C. Dollinger, P. Lavalle, and N.E. Vrana. 2015. Macrophage responses to implants: Prospects for personalized medicine. J Leukoc Biol 98: 953–962.PubMed
37.
38.
Pueyo, M.E., S. Darquy, F. Capron, and G. Reach. 1993. In vitro activation of human macrophages by alginate-polylysine microcapsules. J Biomater Sci Polym Ed 5: 197–203.PubMed
39.
Bygd, H.C., and K.M. Bratlie. 2016. The effect of chemically modified alginates on macrophage phenotype and biomolecule transport. J Biomed Mater Res Part A 104 (7): 1707–1719.
40.
Muñoz, L., A. Albillos, M. Nieto, E. Reyes, L. Lledó, J. Monserrat, E. Sanz, A. Hera, and M. Alvarez-Mon. 2005. Mesenteric Th1 polarization and monocyte TNF-α production: First steps to systemic inflammation in rats with cirrhosis. Hepatology. 42 (2): 411–419.PubMed
41.
42.
Jin, P., Y. Zhao, H. Liu, J. Chen, J. Ren, J. Jin, D. Bedognetti, S. Liu, E. Wang, F. Marincola, and D. Stroncek. 2016. Interferon-γ and tumor necrosis factor-α polarize bone marrow stromal cells uniformly to a Th1 phenotype. Sci Rep 6: 26345.PubMedPubMedCentral
43.
Baeten, D., N. Van Damme, F. Van Den Bosch, E. Kruithof, M. De Vos, and H. Mielants. 2001. Impaired Th1 cytokine production in spondyloarthropathy is restored by anti-TNF. Ann Rheum Dis 60 (8): 750–755.PubMedPubMedCentral
44.
Swain, S.L., and T. Subsets. 1995. T-cell subsets: who does the polarizing? T Curr Biol 5: 849–851.
45.
Kimura, A., and T. Kishimoto. 2010. IL-6: regulator of Treg/Th17 balance. Eur J Immunol 40 (7): 1830–1835.PubMed
46.
Chung, Y., S.H. Chang, G.J. Martinez, X.O. Yang, R. Nurieva, H.S. Kang, L. Ma, S.S. Watowich, A.M. Jetten, Q. Tian, and C. Dong. 2009. Critical regulation of early Th17 cell differentiation by interleukin-1 signaling. Immunity. 30: 576–587.PubMedPubMedCentral
47.
48.
Raval, R.R., A.B. Sharabi, A.J. Walker, C.G. Drake, and P. Sharma. 2014. Tumor immunology and cancer immunotherapy: summary of the 2013 SITC primer. J Immunother Cancer 2: 14.PubMedPubMedCentral
49.
Metadata
Title
Differential Immunomodulatory Effect of Carbon Dots Influenced by the Type of Surface Passivation Agent
Authors
Furkan Ayaz
Melis Ozge Alas
Rukan Genc
Publication date
01-04-2020
Publisher
Springer US
Published in
Inflammation / Issue 2/2020
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-019-01165-0

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