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01-08-2019 | Gingival Diseases | ORIGINAL ARTICLE

Carnosic Acid Inhibits CXCR3 Ligands Production in IL-27-Stimulated Human Oral Epithelial Cells

Authors: Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Takashi Matsuo

Published in: Inflammation | Issue 4/2019

Abstract

Carnosic acid, which is a bioactive compound isolated from rosemary, has various pharmacological effects. However, the anti-inflammatory effect of carnosic acid on periodontitis is still unknown. The aim of this study was to investigate the effect of carnosic acid on CXC chemokine receptor 3 (CXCR3) ligands, which are involved in Th1 cells migration and accumulation, production in interleukin (IL)-27-stimulated human oral epithelial cells (TR146 cells). Carnosic acid decreased CXC chemokine ligand (CXCL)9, CXCL10, and CXCL11 production in IL-27-stimulated TR146 cells in a dose-dependent fashion. Moreover, we disclosed that carnosic acid could suppress signal transducer and activator of transcription (STAT)1, STAT3, and protein kinase B (Akt) phosphorylation in IL-27-stimulated TR146 cells. Furthermore, STAT1, STAT3, and Akt inhibitors could suppress CXCR3 ligands production in IL-27-treated TR146 cells. In summary, carnosic acid could reduce CXCR3 ligands production in human oral epithelial cell by inhibiting STAT1, STAT3, and Akt activation.

Page, R.C. 1991. The role of inflammatory mediators in the pathogenesis of periodontal disease. Journal of Periodontal Research 26: 230–242.CrossRefPubMed

Kawai, T., R. Eisen-Lev, M. Seki, J.W. Eastcott, M.E. Wilson, and M.A. Taubman. 2000. Requirement of B7 costimulation for Th1-mediated inflammatory bone resorption in experimental periodontal disease. Journal of Immunology 64: 2102–2109.CrossRef

Zhang, X., and Y.T. Teng. 2006. Interleukin-10 inhibits gram-negative-microbe-specific human receptor activator of NF-kappaB ligand-positive CD4+-Th1-cell-associated alveolar bone loss in vivo. Infection and Immunity 74: 4927–4931.CrossRefPubMedPubMedCentral

Hienz, S.A., S. Paliwal, and S. Ivanovski. 2015. Mechanisms of bone resorption in periodontitis. Journal of Immunology Research 2015: 615486.CrossRefPubMedPubMedCentral

Heise, C.E., A. Pahuja, S.C. Hudson, M.S. Mistry, A.L. Putnam, M.M. Gross, P.A. Gottlieb, W.S. Wade, M. Kiankarimi, D. Schwarz, P. Crowe, A. Zlotnik, and D.G. Alleva. 2005. Pharmacological characterization of CXC chemokine receptor 3 ligands and a small molecule antagonist. The Journal of Pharmacology and Experimental Therapeutics 313: 1263–1271.CrossRefPubMed

Hosokawa, Y., I. Hosokawa, K. Ozaki, and T. Matsuo. 2017. IL-27 modulates chemokine production in TNF-α -stimulated human oral epithelial cells. Cellular Physiology and Biochemistry 43: 1198–1206.CrossRefPubMed

Thummuri, D., V.G.M. Naidu, and P. Chaudhari. 2017. Carnosic acid attenuates RANKL-induced oxidative stress and osteoclastogenesis via induction of Nrf2 and suppression of NF-κB and MAPK signalling. Journal of Molecular Medicine 95: 1065–1076.CrossRefPubMed

Vázquez, N.M., G. Fiorilli, P.A. Cáceres Guido, and S. Moreno. 2016. Carnosic acid acts synergistically with gentamicin in killing methicillin-resistant Staphylococcus aureus clinical isolates. Phytomedicine. 23: 1337–1343.CrossRefPubMed

Su, K., C.F. Wang, Y. Zhang, Y.J. Cai, Y.Y. Zhang, and Q. Zhao. 2016. The inhibitory effects of carnosic acid on cervical cancer cells growth by promoting apoptosis via ROS-regulated signaling pathway. Biomedicine & Pharmacotherapy 82: 180–191.CrossRef

10.

Gaya, M., V. Repetto, J. Toneatto, C. Anesini, G. Piwien-Pilipuk, and S. Moreno. 2013. Antiadipogenic effect of carnosic acid, a natural compound present in Rosmarinus officinalis, is exerted through the C/EBPs and PPARγ pathways at the onset of the differentiation program. Biochimica et Biophysica Acta 1830: 3796–3806.

11.

Jacobsen, J., E.B. Nielsen, K. Brøndum-Nielsen, M.E. Christensen, H.B. Olin, N. Tommerup, and M.R. Rassing. 1999. Filter-grown TR146 cells as an in vitro model of human buccal epithelial permeability. European Journal of Oral Sciences 107: 138–146.CrossRefPubMed

12.

Garlet, G.P., W. Martins Jr., B.R. Ferreira, C.M. Milanezi, and J.S. Silva. 2003. Patterns of chemokines and chemokine receptors expression in different forms of human periodontal disease. Journal of Periodontal Research 38: 210–217.CrossRefPubMed

13.

Hosokawa, Y., I. Hosokawa, K. Ozaki, and T. Matsuo. 2018. Honokiol and magnolol inhibit CXCL10 and CXCL11 production in IL-27-stimulated human oral epithelial cells. Inflammation 41: 2110–2115.CrossRefPubMed

14.

Hosokawa, Y., I. Hosokawa, K. Ozaki, H. Nakae, and T. Matsuo. 2009 Apr. Cytokines differentially regulate CXCL10 production by interferon-gamma-stimulated or tumor necrosis factor-alpha-stimulated human gingival fibroblasts. Journal of Periodontal Research 44 (2): 225–231.CrossRefPubMed

15.

Hosokawa, Y., I. Hosokawa, K. Ozaki, H. Nakae, and T. Matsuo. 2010. TNFSF14 coordinately enhances CXCL10 and CXCL11 productions from IFN-gamma-stimulated human gingival fibroblasts. Molecular Immunology 47: 666–670.CrossRefPubMed

16.

Hosokawa, Y., I. Hosokawa, K. Ozaki, T. Nakanishi, H. Nakae, and T. Matsuo. 2010 Jul. Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts. The Journal of Nutritional Biochemistry 21 (7): 659–664.CrossRefPubMed

17.

Liu, M., X. Zhou, L. Zhou, Z. Liu, J. Yuan, J. Cheng, J. Zhao, L. Wu, H. Li, H. Qiu, and J. Xu. 2018. Carnosic acid inhibits inflammation response and joint destruction on osteoclasts, fibroblast-like synoviocytes, and collagen-induced arthritis rats. Journal of Cellular Physiology 233: 6291–6303.CrossRefPubMed

18.

Kida, Y., M. Kobayashi, T. Suzuki, A. Takeshita, Y. Okamatsu, S. Hanazawa, T. Yasui, and K. Hasegawa. 2005. Interleukin-1 stimulates cytokines, prostaglandin E2 and matrix metalloproteinase-1 production via activation of MAPK/AP-1 and NF-kappaB in human gingival fibroblasts. Cytokine. 29: 159–168.CrossRefPubMed

19.

Rizzo, A., N. Bevilacqua, L. Guida, M. Annunziata, C. Romano Carratelli, and R. Paolillo. 2012. Effect of resveratrol and modulation of cytokine production on human periodontal ligament cells. Cytokine. 60: 197–204.CrossRefPubMed

20.

Kim, D.H., K.W. Park, I.G. Chae, J. Kundu, E.H. Kim, J.K. Kundu, and K.S. Chun. 2016. Carnosic acid inhibits STAT3 signaling and induces apoptosis through generation of ROS in human colon cancer HCT116 cells. Molecular Carcinogenesis 55: 1096–1110.CrossRefPubMed

21.

Kar, S., S. Palit, W.B. Ball, and P.K. Das. 2012. Carnosic acid modulates Akt/IKK/NF-κB signaling by PP2A and induces intrinsic and extrinsic pathway mediated apoptosis in human prostate carcinoma PC-3 cells. Apoptosis. 17: 735–747.CrossRefPubMed

Title: Carnosic Acid Inhibits CXCR3 Ligands Production in IL-27-Stimulated Human Oral Epithelial Cells
Authors: Ikuko Hosokawa
Yoshitaka Hosokawa
Kazumi Ozaki
Takashi Matsuo
Publication date: 01-08-2019
Publisher: Springer US
Keyword: Gingival Diseases
Published in: Inflammation / Issue 4/2019
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-019-00991-6

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