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01-02-2017 | ORIGINAL ARTICLE

NPS2143 Inhibits MUC5AC and Proinflammatory Mediators in Cigarette Smoke Extract (CSE)-Stimulated Human Airway Epithelial Cells

Authors: Jae-Won Lee, Ji-Won Park, Ok-Kyoung Kwon, Hee Jae Lee, Hye Gwang Jeong, Jae-Hong Kim, Sei-Ryang Oh, Kyoung-Seop Ahn

Published in: Inflammation | Issue 1/2017

Abstract

Mucus overproduction is a fundamental hallmark of COPD that is caused by exposure to cigarette smoke. MUC5AC is one of the main mucin genes expressed in the respiratory epithelium, and its transcriptional upregulation often correlates with increased mucus secretion. Calcium-sensing receptor (CaSR) antagonists have been reported to possess anti-inflammatory effects. The purpose of the present study was to investigate the protective role of NPS2143, a selective CaSR antagonist on cigarette smoke extract (CSE)-stimulated NCI-H292 mucoepidermoid human lung cells. Treatment of NPS2143 significantly inhibited the expression of MUC5AC in CSE-stimulated H292 cells. NPS2143 reduced the expression of MMP-9 in CSE-stimulated H292 cells. NPS2143 also decreased the release of proinflammatory cytokines such as IL-6 and TNF-α in CSE-stimulated H292 cells. Furthermore, NPS2143 attenuated the activation of MAPKs (JNK, p38, and ERK) and inhibited the nuclear translocation of NF-κB in CSE-stimulated H292 cells. These results indicate that NPS2143 had a therapeutic potential in COPD.

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Title: NPS2143 Inhibits MUC5AC and Proinflammatory Mediators in Cigarette Smoke Extract (CSE)-Stimulated Human Airway Epithelial Cells
Authors: Jae-Won Lee
Ji-Won Park
Ok-Kyoung Kwon
Hee Jae Lee
Hye Gwang Jeong
Jae-Hong Kim
Sei-Ryang Oh
Kyoung-Seop Ahn
Publication date: 01-02-2017
Publisher: Springer US
Published in: Inflammation / Issue 1/2017
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-016-0468-2

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Other articles of this Issue 1/2017

Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells

Inhibition of IRF8 Negatively Regulates Macrophage Function and Impairs Cutaneous Wound Healing

Role of Leptin and SOCS3 in Inhibiting the Type I Interferon Response During Obesity

Indirubin Inhibits LPS-Induced Inflammation via TLR4 Abrogation Mediated by the NF-kB and MAPK Signaling Pathways

Porphyromonas gingivalis Lipopolysaccharide Induces a Pro-inflammatory Human Gingival Fibroblast Phenotype

The Diverse Biological Functions of Neutrophils, Beyond the Defense Against Infections

ACC 2024 Congress Coverage

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