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01-12-2016 | ORIGINAL ARTICLE

Maresin 1 Maintains the Permeability of Lung Epithelial Cells In Vitro and In Vivo

Authors: Lin Chen, Hong Liu, Yaxin Wang, Haifa Xia, Jie Gong, Bo Li, Shanglong Yao, You Shang

Published in: Inflammation | Issue 6/2016

Abstract

Previous reports showed that Maresin 1 (MaR1) possessed organ protection effects and could attenuate acute lung injury. Here, we aim to figure out whether MaR1 can maintain the permeability of lung epithelial cells by regulating the expression of tight junction protein during lung injury. Monolayer of murine lung epithelial cells was stimulated by lipopolysaccharide (LPS) with or without MaR1 and the permeability was evaluated. The expression of Claudin-1 and ZO-1 in lung epithelial cells was analyzed by immunofluorescence staining and western blotting. MaR1 was given to the mice after LPS induced acute lung injury. The permeability of lung was assessed by Evans Blue extravasation, lung wet/dry ratio and protein concentration in bronchoalveolar lavage fluid. Lung injury score was also evaluated. The expression of Claudin-1 and ZO-1 in the lung was analyzed by immunofluorescence staining. Results showed that MaR1 maintained the permeability of lung epithelial cells and upregulated the expression of Claudin-1 and ZO-1 after LPS stimulation. In acute lung injury mice, MaR1 upregulated the expression of Claudin-1 and ZO-1, decreased lung permeability, and reduced lung injury. In summary, this study suggests that MaR1 can maintain the permeability of lung epithelial cells by upregulating the expression of Claudin-1 and ZO-1 in acute lung injury.

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Title: Maresin 1 Maintains the Permeability of Lung Epithelial Cells In Vitro and In Vivo
Authors: Lin Chen
Hong Liu
Yaxin Wang
Haifa Xia
Jie Gong
Bo Li
Shanglong Yao
You Shang
Publication date: 01-12-2016
Publisher: Springer US
Published in: Inflammation / Issue 6/2016
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-016-0433-0

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