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01-12-2014

Effects of PPAR-γ Agonist Treatment on LPS-Induced Mastitis in Rats

Authors: Ding Mingfeng, Ming Xiaodong, Liu Yue, Piao Taikui, Xiao Lei, Liu Ming

Published in: Inflammation | Issue 6/2014

Abstract

PPAR-γ, a member of the nuclear receptor superfamily, plays an important role in lipid metabolism and inflammation. The aim of this study was to investigate the preventive effects of synthetic PPAR-γ agonist rosiglitazone on lipopolysaccharide (LPS)-induced mastitis in rats. The mouse model of mastitis was induced by the injection of LPS through the duct of the mammary gland. Rosiglitazone was injected 1 h before the induction of LPS intraperitoneally. The results showed that rosiglitazone attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO), and the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in a dose-dependent manner. Additionally, Western blotting showed that rosiglitazone inhibited the phosphorylation of IκB-α and NF-κB p65. These results indicated that rosiglitazone has a protective effect on mastitis, and the anti-inflammatory mechanism of rosiglitazone on LPS-induced mastitis in rats may be due to its ability to inhibit NF-κB signaling pathways. PPAR-γ may be a potential therapeutic target against mastitis.

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Title: Effects of PPAR-γ Agonist Treatment on LPS-Induced Mastitis in Rats
Authors: Ding Mingfeng
Ming Xiaodong
Liu Yue
Piao Taikui
Xiao Lei
Liu Ming
Publication date: 01-12-2014
Publisher: Springer US
Published in: Inflammation / Issue 6/2014
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-014-9924-z

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