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Inflammation
Published in: Inflammation 2/2013

01-04-2013

Evaluation of CHI3L-1 and CHIT-1 Expression in Differentiated and Polarized Macrophages

Authors: Michelino Di Rosa, Giulia Malaguarnera, Corinne De Gregorio, Filippo Drago, Lucia Malaguarnera

Published in: Inflammation | Issue 2/2013

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Abstract

Chitinase 3-like protein 1 (CHI3L-1) and chitotriosidase (CHIT-1) are members of the chitinase family. CHI3L-1 is a newly recognized protein that is secreted by activated macrophages and neutrophils and expressed in a broad spectrum of inflammatory conditions and cancers. In human plasma, CHIT-1 activity has been proposed as a biochemical marker of macrophage activation. Although CHI3L-1 expression in inflammation is under examination, little is known regarding its regulation during macrophages’ full maturation and polarization. In this study, we compared CHI3L-1 and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR. We found that during the maturation of monocytes into macrophages, the expression of both CHI3L-1 and CHIT-1 increased exponentially over time. Additionally, we observed a different regulation of CHI3L-1 and CHIT-1 in undifferentiated monocytes under stimulation with lipopolysaccharide, interferon-γ, and interleukin-4, at the same concentration used to polarize macrophages. Our finding suggests that in the immune response, the role of CHI3L-1 and CHIT-1 is not restricted to innate immunity, but they are also protagonists in acquired immunity.
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Metadata
Title
Evaluation of CHI3L-1 and CHIT-1 Expression in Differentiated and Polarized Macrophages
Authors
Michelino Di Rosa
Giulia Malaguarnera
Corinne De Gregorio
Filippo Drago
Lucia Malaguarnera
Publication date
01-04-2013
Publisher
Springer US
Published in
Inflammation / Issue 2/2013
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-012-9569-8

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