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Published in:

01-08-2012

Expression of Complement Components and Regulators by Different Subtypes of Bone Marrow-Derived Macrophages

Authors: Chang Luo, Mei Chen, Angelina Madden, Heping Xu

Published in: Inflammation | Issue 4/2012

Abstract

Under inflammatory conditions, macrophages can differentiate into different functional subtypes. We show that bone marrow-derived macrophages constitutively express different levels of various complement-related genes. The relative expression levels are C1qb > Crry > CFH > C3 > C1r > CFB > DAF1 > CD59a > C2 > C1INH > C1s > C4. Upon activation, the expression of C1r, C1s, C3, C2, CFB, and C1INH was up-regulated, and CFH, CD59a, and DAF1, down-regulated in M1 (induced by interferon-γ + lipopolysaccharides (LPS)) and M2b (induced by immune complex + LPS) macrophages. The expression of C4 and CFH was slightly up-regulated in interleukin (IL)-10-induced M2c macrophages. Complement gene expression in IL-4-induced M2a macrophages was weakly down-regulated as compared to resting M0 macrophages. Higher levels of C3, C1INH, and CFB but lower levels of CFH expression in M1 and M2b macrophage suggests that they may be involved in the alternative pathway of complement activation during inflammation.

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Title: Expression of Complement Components and Regulators by Different Subtypes of Bone Marrow-Derived Macrophages
Authors: Chang Luo
Mei Chen
Angelina Madden
Heping Xu
Publication date: 01-08-2012
Publisher: Springer US
Published in: Inflammation / Issue 4/2012
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-012-9458-1

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