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Inflammation
Published in: Inflammation 1-2/2007

01-04-2007

Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"

Authors: Elizabeth Fireman, Moshe Shtark, Israel E. Priel, Robert Shiner, Ram Mor, Shmuel Kivity, Zvi Fireman

Published in: Inflammation | Issue 1-2/2007

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Abstract

We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20’ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined.
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Metadata
Title
Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"
Authors
Elizabeth Fireman
Moshe Shtark
Israel E. Priel
Robert Shiner
Ram Mor
Shmuel Kivity
Zvi Fireman
Publication date
01-04-2007
Published in
Inflammation / Issue 1-2/2007
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-007-9020-8

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