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Documenta Ophthalmologica

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01-12-2012 | Original Research Article

Response properties of slow PIII in the Large ^vls mutant

Authors: Neal S. Peachey, Gwen M. Sturgill-Short

Published in: Documenta Ophthalmologica | Issue 3/2012

Abstract

Purpose

Mouse mutants for proteins expressed in the dystrophin–glycoprotein complex at the photoreceptor terminal have electroretinogram (ERG) b-waves with a delayed onset and time course. The b-wave is defined by the sum of PII generated by depolarizing bipolar cells and slow PIII generated by Müller glial cells. In this study, we evaluated the hypothesis that the abnormalities observed in one of these mutants, Large ^vls, are caused by abnormal response properties of slow PIII.

Methods

To isolate slow PIII, we crossed the Large ^vls mutant to a mouse line (Gpr179 ^nob5) that lacks the ERG b-wave but maintains normal photoreceptor function and in which retinal degeneration does not occur. ERGs were recorded to strobe flash stimuli after overnight dark adaptation.

Results

In comparison with control responses, the a-wave and slow PIII had comparable waveforms but were reduced in amplitude in Large ^vls mice. The magnitude of this reduction was comparable for these components, and across stimulus luminance. There was no stimulus condition where the amplitude of slow PIII was larger than control.

Conclusions

The data obtained are inconsistent with the idea that the b-wave abnormalities noted in Large ^vls mutant mice are caused by abnormal response properties of slow PIII.

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Title: Response properties of slow PIII in the Large vls mutant
Authors: Neal S. Peachey
Gwen M. Sturgill-Short
Publication date: 01-12-2012
Publisher: Springer-Verlag
Published in: Documenta Ophthalmologica / Issue 3/2012
Print ISSN: 0012-4486
Electronic ISSN: 1573-2622
DOI: https://doi.org/10.1007/s10633-012-9347-9

At a glance: The STEP trials

Springer Medicine

Response properties of slow PIII in the Large ^vls mutant

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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