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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 3/2020

01-03-2020 | Review

Tools for Analysis of the Microbiome

Authors: Jessica Galloway-Peña, Blake Hanson

Published in: Digestive Diseases and Sciences | Issue 3/2020

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Abstract

Over the past decade, it has become exceedingly clear that the microbiome is a critical factor in human health and disease and thus should be investigated to develop innovative treatment strategies. The field of metagenomics has come a long way in leveraging the advances of next-generation sequencing technologies resulting in the capability to identify and quantify all microorganisms present in human specimens. However, the field of metagenomics is still in its infancy, specifically in regard to the limitations in computational analysis, statistical assessments, standardization, and validation due to vast variability in the cohorts themselves, experimental design, and bioinformatic workflows. This review summarizes the methods, technologies, computational tools, and model systems for characterizing and studying the microbiome. We also discuss important considerations investigators must make when interrogating the involvement of the microbiome in health and disease in order to establish robust results and mechanistic insights before moving into therapeutic design and intervention.
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Metadata
Title
Tools for Analysis of the Microbiome
Authors
Jessica Galloway-Peña
Blake Hanson
Publication date
01-03-2020
Publisher
Springer US
Published in
Digestive Diseases and Sciences / Issue 3/2020
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-020-06091-y

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