Top

Published in:

01-07-2008 | Original Paper

Kampo Medicine “Dai-Kenchu-To” Prevents Bacterial Translocation in Rats

Authors: Kozo Yoshikawa, Nobuhiro Kurita, Jun Higashijima, Tomohiko Miyatani, Hidenori Miyamoto, Masanori Nishioka, Mitsuo Shimada

Published in: Digestive Diseases and Sciences | Issue 7/2008

Abstract

Kampo medicine “Dai-kenchu-to” (DKT) has been used for treatment of ileus. The aim of this study was to evaluate the role of DKT on the bacterial translocation (BT) model in rats. Rats were divided into the following four groups: group 1, receiving only water, and groups 2, 3, and 4, receiving 100, 300, and 1,000 mg/kg/day of DKT. Rats were sacrificed 6 days after the beginning of the fast, and then the mesenteric lymph node was cultured. Inflammatory cytokines, intestinal integrity, and apoptosis were assessed. Incidence of BT in groups 3 (33%) and 4 (16%) was lower than in group 1 (66%). Interferon-γ expression in groups 2, 3, and 4 was significantly lower than in group 1. Villous height and number of villus in groups 2, 3, and 4 were significantly taller and greater than in group 1. Apoptotic index in groups 2, 3, and 4 was significantly lower than in group 1. This is the first evidence that DKT prevents BT by reducing inflammatory reaction and maintaining intestinal integrity.

MacFie J, Reddy BS, Gatt M, Jain PK, Sowdi R, Mitchell CJ (2006) Bacterial translocation studied in 927 patients over 13 years. Brit J Surg 93:87–93PubMedCrossRef

Anderson ADG, McNaught CE, Jain PK, MacFie J (2004) Randomised clinical trial of symbiotic therapy in elective surgical patients. Gut 53:241–245PubMedCrossRef

Ekelund M, Ekelund M, Qader SS, Hallen MH, Ekblad E (2005) Effects of total parenteral nutrition on rat enteric nervous system, intestinal morphology, and motility. J Surg Res 124:187–193PubMedCrossRef

Wildhaber BE, Yang H, Spencer AU, Drongowski RA, Teitelbaum DH (2005) Lack of enteral nutrition—effects on the intestinal immune system. J Surg Res 123:8–16PubMedCrossRef

Warner BW (2003) Enterocyte apoptosis and TPN-associated intestinal mucosal atrophy: a view of the chicken or the egg? Gastroenterology 125:1273–1274PubMedCrossRef

Yang H, Fan Y, Teitelbaum DH (2003) Intraepithelial lymphocyte-derived interferon-γ evokes enterocyte apoptosis with parenteral nutrition in mice. Am J Physiol Gastrointest Liver Physiol 284:629–637

Veal N, Auduberteau H, Lemarie C, Oberti F, Cales P (2001) Effect of octreotide on intestinal transit and bacterial translocation in conscious rats with portal hypertension and liver fibrosis. Digest Dis Sci 46:2367–2373PubMedCrossRef

Sato Y, Inoue S, Katagiri F, Itoh H, Takeyama M (2006) Effects of pirenzepine on Dai-kenchu-to-induced elevation of the plasma neuropeptide levels in humans. Biol Pharm Bull 29:166–171PubMedCrossRef

Satoh K, Kase Y, Yuzurihara M, Mizoguchi K, Kurauchi K, Ishige A (2003) Effect of Dai-kenchu-to (Da-Jian-Zhong-Tang) on the delayed intestinal propulsion induced by chlorpromazine in mice. J Ethnopharmacol 86:37–44PubMedCrossRef

10.

Hashimoto K, Satoh K, Murata P, Makino B, Sakakibara I, Kase Y, Ishige A, Higuchi M, Sasaki H (2003) Components of Panax ginseng that improve accelerated small intestinal transit. J Ethnopharmacol 84:115–119PubMedCrossRef

11.

Nakamura T, Sakai A, Isogami I, Noda K, Ueno K, Yano S (2002) Abatement of morphine-induced slowing in gastrointestinal transit by Dai-kenchu-to, a traditional Japanese herbal medicine. Jpn J Pharmacol 88:217–221PubMedCrossRef

12.

Satoh K, Hashimoto K, Hayakawa T, Ishige A, Kaneko M, Ogihara S, Kurosawa S, Yakabi K, Nakamura T (2001) Mechanism of atropine-resistant contraction induced by Dai-kenchu-to in guinea pig ileum. Jpn J Pharmacol 86:32–37PubMedCrossRef

13.

Suehiro T, Matsumata T, Shikada Y, Sugimachi K (2005) The effect of the herbal medicines Dai-kenchu-to and Keishi-bukuryo-gan on bowel movement after colorectal surgery. Hepato-gastroenterol 52:97–100PubMed

14.

Itoh T, Yamakawa J, Mai M, Yamaguchi N, Kanda T (2002) The effect of the herbal medicine Dai-kenchu-to on post-operative ileus. J Int Med Res 30:428–432PubMed

15.

Hayakawa T, Kase Y, Saito K, Hashimoto K, Ishige A, Komatsu Y, Sasaki H (1999) Pharmacological studies of the effect of Dai-kenchu-to on spontaneous contraction of isolated rabbit jejunum. J Smooth Muscle Res 35:55–62PubMed

16.

Murata P, Kase Y, Ishige A, Sasaki H, Kurosawa S, Nakamura T (2002) The herbal medicine Dai-kenchu-to and one of its active components 6-shogaol increase intestinal blood flow in rats. Life Sci 70:2061–2070PubMedCrossRef

17.

Kaiho T, Tanaka T, Tsuchiya S, Yanagisawa S, Takeuchi O, Miura M, Saigusa N, Miyazaki M (2004) Effect of the herbal medicine Dai-kenchu-to for serum ammonia in hepatectomized patient. Hepato-gastroenterol 52:161–165

18.

Hayakawa T, Kase Y, Saito K, Kashimoto K, Ishige A, Komatsu Y, Sasaki H (1999) Effect of Dai-kenchu-to on intestinal obstruction following laparotomy. J Smooth Muscle Res 35:47–54PubMed

19.

Endo S, Nishida T, Nishikawa K, Nakajima K, Hasegawa J, Kitagawa T, Ito T, Matsuda H (2006) Dai-kenchu-to, a Chinese herbal medicine, improves stasis of patients with total gastrectomy and jejunal pouch interposition. Am J Surg 192:9–13PubMedCrossRef

20.

Sato Y, Katagiri F, Inoue S, Itoh H, Takeyama M (2004) Dai-kenchu-to raises levels of calcitonin gene-related peptide and substance P in human plasma. Biol Pharm Bull 27:1875–1877PubMedCrossRef

21.

Fukuda H, Chen C, Mantyh C, Ludwig K, Pappas TN, Takehashi T (2006) The herbal medicine, Dai-Kenchu-To, accelerates delayed gastrointestinal transit after the operation in rats. J Surg Res 131:290–295PubMedCrossRef

22.

Llpis M, Antolin M, Guarner F, Salas A, Malagelada JR (2005) Mucosal colonization with Lactobacillus casei mitigates barrier injury induced by exposure to trinitronbenzene sulphonic acid. Gut 54:955–959CrossRef

23.

Zou Y, Hernandez F, Burgos E, Martinez L, Gonzalez-Reyes S, Fernandez-Dumont V, Lopez G, Romero M, Lopez-Santamaria M, Tovar JA (2005) Bacterial translocation in acute rejection after small bowel transplantation in rats. Pediatr Surg Int 21:208–211PubMedCrossRef

24.

Tayfum Gurbuz A, Kunzelman J, Ratzer EE (1998) Supplemental dietary arginine accelerates intestinal mucosal regeneration and enhances bacterial clearance following radiation enteritis in rats. J Surg Res 74:149–154CrossRef

25.

Demirer S, Aydintug S, Aslim B, Kepenekci I, Sengui N, Evirgen O, Gerceker D, Andrieu MN, Ulusoy C, Karahuseyinoglu S (2006) Effects of probiotics on radiation-induced intestinal injury in rats. Nutrition 22:179–186PubMedCrossRef

26.

Iskit SH, Tugtepe H, Ayyildiz SH, Kotiloglu E, Dagli TE, Yegen BC (2005) Epidermal growth factor and bombesin act synergistically to support intestinal adaptation in rats with massive small bowel resenction. Pediatr Surg Int 21:436–440PubMedCrossRef

27.

Hang CH, Shi JX, Sun BW, Li JS (2006) Apoptosis and functional changes of dipeptide transporter in the rat small intestine after traumatic brain injury. J Surg Res 137:53–60PubMedCrossRef

28.

Zareie M, Johnson-Henry K, Jury J, Yang P-C, Ngan B-Y, McKay DM, Soderholm JD, Perdue MH, Sherman PM (2006) Probiotics prevent bacterial translation and improve intestinal barrier function in rats following chronic psychological stress. Gut 55:1553–1560PubMedCrossRef

29.

Yasuda T, Takeyama Y, Ueda T, Shinzeki M, Sawa H, Nakajima T, Kuroda Y (2006) Breakdown of intestinal mucosa via accelerated apoptosis increases intestinal permeability in experimental severe acute pancreatitis. J Surg Res 135:18–26PubMedCrossRef

30.

Tsuchioka T, Fujiwara T, Sunagawa M (2006) Effects of glutamic asid and taurine on total parenteral nutrition. J Pediatr Surg 41:1566–1572PubMedCrossRef

31.

Sun X, Spencer AU, Yang H, Haxhija EQ, Teitelbaum DH (2006) Impact of caloric intake on parenteral nutrition-associated intestinal morphology and mucosal barrier function. J Parenter Enteral Nutr 30:474–479CrossRef

32.

Kiristioglu I, Teitelbaum DH (1998) Alteration of the intestinal intraepithelial lymphocytes during total parenteral nutrition. J Surg Res 79:91–96PubMedCrossRef

33.

Yang H, Kiristioglu I, Fan Y, Forbush B, Bishop K, Antony PA, Zhou H, Teitelbaum DH (2002) Interferon-gamma expression by intraepithelial lymphocytes results in a loss of epithelial barrier function in a mouse model of total perenteral nutrition. Ann Surg 2:226–234CrossRef

34.

Clark E, Hoare C, Tanianis-Hughes J, Carlson G, Warhurst G (2005) Interferon induced translocation of commemsal escherichia coli across gut epithelial cells via a lipid raft-mediated process. Gastroenterology 128:1258–1267PubMedCrossRef

35.

Resta LS, Barrett KE (2006) Probiotics and commensals reverse TNF-α and IFN-γ induced dysfunction in human intestinal epithelial cells. Gastroenterology 130:731–746CrossRef

36.

Furrie E, Macfarlane S, Kennedy K, Cummings H, Walsh SV, O’Neil DA, Macfarlane GT (2005) Synbiotic theory (Bifidobacterium longum/Synergy1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomized controlled pilot trial. Gut 54:242–249PubMedCrossRef

37.

Gunji H, Scarth S, Carlson GL, Warhurt G, Little RA, Hopkins SJ (2006) Variability of bacterial translocation in the absence of intestinal mucosal damage following injury and the influence of interleukin-6. Pathophysiology 13:39–49PubMedCrossRef

38.

Ohya T, Usui Y, Arii S, Iwai T (2003) Effect of Dai-kenchu-to on obstructive bowel disease in children. Am J Chinese Med 31:129-135PubMedCrossRef

39.

Jin X, Shibata C, Naito H, Ueno T, Funayama Y, Fukushima K, Matsuno S, Sasaki I (2001) Intraduodenal and intrajejunal administration of the herbal medicine, Dai-Kenchu-Tou, stimulates small intestinal motility via cholinergic receptors in conscious dogs. Digest Dis Sci 46:1171–1176PubMedCrossRef

40.

Satoh K, Hayakawa T, Kase Y, Ishige A, Sasaki H, Nishikawa S, Kurosawa S, Yakabi K, Nakamura T (2001) Mechanisms for contractile effect of Dai-kenchu-to in isolated guinea pig ileum. Digest Dis Sci 46:250–256PubMedCrossRef

41.

Shibata C, Sasaki I, Naito H, Ueno T, Matsumo S (1999) The herbal medicine Dai-kenchu-tou stimulates upper gut motility through cholinergic and 5-hydroxytryptamine 3 receptors in conscious dogs. Surgery 126:918–924PubMed

Title: Kampo Medicine “Dai-Kenchu-To” Prevents Bacterial Translocation in Rats
Authors: Kozo Yoshikawa
Nobuhiro Kurita
Jun Higashijima
Tomohiko Miyatani
Hidenori Miyamoto
Masanori Nishioka
Mitsuo Shimada
Publication date: 01-07-2008
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 7/2008
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0281-3

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Kampo Medicine “Dai-Kenchu-To” Prevents Bacterial Translocation in Rats

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 7/2008

Li-cadherin is Inversely Correlated with Galectin-3 Expression in Gastric Cancer

Association of Gluten Enteropathy and Irritable Bowel Syndrome in Adult Turkish Population

Evaluation of Gastric Cancer Risk Using Topography of Histological Gastritis: A Large-scaled Cross-sectional Study

Akt1/protein Kinase Bα is Involved in Gastric Cancer Progression and Cell Proliferation

Consensus Interferon and Ribavirin in Patients with Chronic Hepatitis C Who Were Nonresponders to Pegylated Interferon alfa-2b and Ribavirin

A Unique Presentation: Dorsal Agenesis of the Pancreas Manifesting as Pancreatic Exocrine Insufficiency in the Absence of Diabetes Mellitus in an 8-Year-Old Boy

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page