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Cardiovascular Drugs and Therapy
Published in: Cardiovascular Drugs and Therapy 6/2023

16-02-2022 | Myocardial Infarction | Review Article

Depletion of microRNA-92a Enhances the Role of Sevoflurane Treatment in Reducing Myocardial Ischemia–Reperfusion Injury by Upregulating KLF4

Authors: Qianfu Wu, Haihui Wang, Fei He, Jiali Zheng, Hongjing Zhang, Chang Cheng, Panwei Hu, Rong Lu, Guoliang Yan

Published in: Cardiovascular Drugs and Therapy | Issue 6/2023

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Abstract

Objective

As some articles have highlighted the role of microRNA-92a (miR-92a) in myocardial ischemia–reperfusion injury (MI/RI), this article aimed to investigate the effect of miR-92a on Sevoflurane (Sevo)-treated MI/RI via regulation of Krüppel-like factor 4 (KLF4).

Methods

An MI/RI rat model was established by ligating the left anterior descending coronary artery. The cardiac function, pathological changes of myocardial tissues, inflammatory response, oxidative stress and cardiomyocyte apoptosis in MI/RI rats were determined. KLF4 and miR-92a expression was detected in the myocardial tissue of rats, and the target relationship between miR-92a and KLF4 was confirmed.

Results

Sevo treatment alleviated myocardial damage, inflammatory response, oxidative stress response, and cardiomyocyte apoptosis, and improved cardiac function in MI/RI rats. miR-92a increased and KLF4 decreased in the myocardial tissue of MI/RI rats. KLF4 was targeted by miR-92a. Downregulation of miR-92a or upregulation of KLF4 further enhanced the effect of Sevo treatment on MI/RI.

Conclusion

This study suggests that depletion of miR-92a promotes upregulation of KLF4 to improve cardiac function, reduce cardiomyocyte apoptosis and further enhance the role of Sevo treatment in alleviating MI/RI.
Appendix
Available only for authorised users
Literature
1.
Yu SY, et al. Knockdown of lncRNA AK139328 alleviates myocardial ischaemia/reperfusion injury in diabetic mice via modulating miR-204-3p and inhibiting autophagy. J Cell Mol Med. 2018;22(10):4886–98.CrossRefPubMedPubMedCentral
2.
3.
4.
Ma N, et al. Trimetazidine protects against cardiac ischemia/reperfusion injury via effects on cardiac miRNA21 expression, Akt and the Bcl2/Bax pathway. Mol Med Rep. 2016;14(5):4216–22.CrossRefPubMedPubMedCentral
5.
Chen JG, et al. Inhibiting miR-155 protects against myocardial ischemia/reperfusion injury via targeted regulation of HIF-1alpha in rats. Iran J Basic Med Sci. 2019;22(9):1050–8.PubMedPubMedCentral
6.
7.
Yu H, et al. MiR-92a modulates proliferation, apoptosis, migration, and invasion of osteosarcoma cell lines by targeting Dickkopf-related protein 3. Biosci Rep. 2019;39:4.CrossRef
8.
Qiao SG, et al. Sevoflurane postconditioning protects against myocardial ischemia/reperfusion injury by restoring autophagic flux via an NO-dependent mechanism. Acta Pharmacol Sin. 2019;40(1):35–45.CrossRefPubMed
9.
Huang HT, et al. MiR-92a regulates endothelial progenitor cells (EPCs) by targeting GDF11 via activate SMAD2/3/FAK/Akt/eNOS pathway. Ann Transl Med. 2019;7(20):563.CrossRefPubMedPubMedCentral
10.
Cheng M, An S, Li J. CDKN2B-AS may indirectly regulate coronary artery disease-associated genes via targeting miR-92a. Gene. 2017;629:101–7.CrossRefPubMed
11.
Ye Y, et al. The role of microRNA in modulating myocardial ischemia-reperfusion injury. Physiol Genomics. 2011;43(10):534–42.CrossRefPubMed
12.
Zhang B, et al. MicroRNA-92a inhibition attenuates hypoxia/reoxygenation-induced myocardiocyte apoptosis by targeting Smad7. PLoS One. 2014;9(6):e100298.CrossRefPubMedPubMedCentral
13.
Liu H, et al. Inhibition of MiR-92a may protect endothelial cells after acute myocardial infarction in rats: role of KLF2/4. Med Sci Monit. 2016;22:2451–62.CrossRefPubMedPubMedCentral
14.
Sakaizawa T, et al. Potential role of ASC, a Proapoptotic protein, for determining the Cisplatin susceptibility of lung Cancer cells. Tohoku J Exp Med. 2018;244(2):133–44.CrossRefPubMed
15.
Zhang Y, et al. Kruppel-like factor 4 transcriptionally regulates TGF-beta1 and contributes to cardiac myofibroblast differentiation. PLoS One. 2013;8(4):e63424.CrossRefPubMedPubMedCentral
16.
Yoshida T, et al. Kruppel-like factor 4 protein regulates isoproterenol-induced cardiac hypertrophy by modulating myocardin expression and activity. J Biol Chem. 2014;289(38):26107–18.CrossRefPubMedPubMedCentral
17.
Ferdinandy P, Schulz R, Baxter GF. Interaction of cardiovascular risk factors with myocardial ischemia/reperfusion injury, preconditioning, and postconditioning. Pharmacol Rev. 2007;59(4):418–58.CrossRefPubMed
18.
Qin X, et al. Exosomal miR-196a derived from cancer-associated fibroblasts confers cisplatin resistance in head and neck cancer through targeting CDKN1B and ING5. Genome Biol. 2019;20(1):12.CrossRefPubMedPubMedCentral
19.
20.
Tian ZQ, Jiang H, Lu ZB. MiR-320 regulates cardiomyocyte apoptosis induced by ischemia-reperfusion injury by targeting AKIP1. Cell Mol Biol Lett. 2018;23:41.CrossRefPubMedPubMedCentral
21.
Yu P, et al. Protective effect of Sevoflurane Postconditioning against cardiac ischemia/reperfusion injury via ameliorating mitochondrial impairment, oxidative stress and rescuing Autophagic clearance. PLoS One. 2015;10(8):e0134666.CrossRefPubMedPubMedCentral
22.
Hinkel R, et al. Inhibition of microRNA-92a protects against ischemia/reperfusion injury in a large-animal model. Circulation. 2013;128(10):1066–75.CrossRefPubMed
23.
24.
Jiang C, et al. The effects and mechanism of miR-92a and miR-126 on myocardial apoptosis in mouse ischemia-reperfusion model. Cell Biochem Biophys. 2014;70(3):1901–6.CrossRefPubMed
25.
26.
Wang W, et al. Circulating microRNA-92a level predicts acute coronary syndrome in diabetic patients with coronary heart disease. Lipids Health Dis. 2019;18(1):22.CrossRefPubMedPubMedCentral
27.
Zhu Z, et al. Kruppel-like factor 4 inhibits pancreatic Cancer epithelial-to-Mesenchymal transition and metastasis by Down-regulating Caveolin-1 expression. Cell Physiol Biochem. 2018;46(1):238–52.CrossRefPubMed
28.
Fan L, et al. Astragalus polysaccharide ameliorates lipopolysaccharide-induced cell injury in ATDC5 cells via miR-92a/KLF4 mediation. Biomed Pharmacother. 2019;118:109180.CrossRefPubMed
29.
Liu PJ, et al. MiRNA-92a promotes cell proliferation and invasion through binding to KLF4 in glioma. Eur Rev Med Pharmacol Sci. 2019;23(15):6612–20.PubMed
30.
Wu J, et al. Sevoflurane postconditioning protects the myocardium against ischemia/reperfusion injury via activation of the JAK2-STAT3 pathway. PeerJ. 2017;5:e3196.CrossRefPubMedPubMedCentral
31.
Cao J, et al. Sevoflurane post-conditioning reduces rat myocardial ischemia reperfusion injury through an increase in NOS and a decrease in phopshorylated NHE1 levels. Int J Mol Med. 2015;36(6):1529–37.CrossRefPubMedPubMedCentral
32.
Dong J, et al. Effects of Sevoflurane pretreatment on myocardial ischemia-reperfusion injury through the Akt/hypoxia-inducible factor 1-alpha (HIF-1alpha)/vascular endothelial growth factor (VEGF) signaling pathway. Med Sci Monit. 2019;25:3100–7.CrossRefPubMedPubMedCentral
33.
Yu R, et al. Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-beta1-meidated ERK/JNK/NF-kappaB signaling pathways. Tumour Biol. 2017;39(6):1010428317705574.CrossRefPubMed
34.
Wang L, et al. Role of Kruppel-like factor 4 in regulating inhibitor of apoptosis-stimulating protein of p53 in the progression of gastric cancer. Oncol Lett. 2018;15(5):6865–72.PubMedPubMedCentral
Metadata
Title
Depletion of microRNA-92a Enhances the Role of Sevoflurane Treatment in Reducing Myocardial Ischemia–Reperfusion Injury by Upregulating KLF4
Authors
Qianfu Wu
Haihui Wang
Fei He
Jiali Zheng
Hongjing Zhang
Chang Cheng
Panwei Hu
Rong Lu
Guoliang Yan
Publication date
16-02-2022
Publisher
Springer US
Published in
Cardiovascular Drugs and Therapy / Issue 6/2023
Print ISSN: 0920-3206
Electronic ISSN: 1573-7241
DOI
https://doi.org/10.1007/s10557-021-07303-x

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