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Published in: Angiogenesis 4/2018

01-11-2018 | Original Paper

Heparin impairs angiogenic signaling and compensatory lung growth after left pneumonectomy

Authors: Duy T. Dao, Lorenzo Anez-Bustillos, Jared Ourieff, Amy Pan, Paul D. Mitchell, Hiroko Kishikawa, Gillian L. Fell, Meredith A. Baker, Randolph S. Watnick, Hong Chen, Thomas E. Hamilton, Michael S. Rogers, Diane R. Bielenberg, Mark Puder

Published in: Angiogenesis | Issue 4/2018

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Abstract

Children with hypoplastic lung diseases, such as congenital diaphragmatic hernia, can require life support via extracorporeal membrane oxygenation and systemic anticoagulation, usually in the form of heparin. The role of heparin in angiogenesis and organ growth is inconclusive, with conflicting data reported in the literature. This study aimed to investigate the effects of heparin on lung growth in a model of compensatory lung growth (CLG). Compared to the absence of heparin, treatment with heparin decreased the vascular endothelial growth factor (VEGF)-mediated activation of VEGFR2 and mitogenic effect on human lung microvascular endothelial cells in vitro. Compared to non-heparinized controls, heparinized mice demonstrated impaired pulmonary mechanics, decreased respiratory volumes and flows, and reduced activity levels after left pneumonectomy. They also had lower lung volume, pulmonary septal surface area and alveolar density on morphometric analyses. Lungs of heparinized mice displayed decreased phosphorylation of VEGFR2 compared to the control group, with consequential downstream reduction in markers of cellular proliferation and survival. The use of bivalirudin, an alternative anticoagulant that does not interact with VEGF, preserved lung growth and pulmonary mechanics. These results demonstrated that heparin impairs CLG by reducing VEGFR2 activation. These findings raise concern for the clinical use of heparin in the setting of organ growth or regeneration.
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Metadata
Title
Heparin impairs angiogenic signaling and compensatory lung growth after left pneumonectomy
Authors
Duy T. Dao
Lorenzo Anez-Bustillos
Jared Ourieff
Amy Pan
Paul D. Mitchell
Hiroko Kishikawa
Gillian L. Fell
Meredith A. Baker
Randolph S. Watnick
Hong Chen
Thomas E. Hamilton
Michael S. Rogers
Diane R. Bielenberg
Mark Puder
Publication date
01-11-2018
Publisher
Springer Netherlands
Published in
Angiogenesis / Issue 4/2018
Print ISSN: 0969-6970
Electronic ISSN: 1573-7209
DOI
https://doi.org/10.1007/s10456-018-9628-3

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