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01-05-2019 | Original Paper

Live imaging of angiogenesis during cutaneous wound healing in adult zebrafish

Authors: Chikage Noishiki, Shinya Yuge, Koji Ando, Yuki Wakayama, Naoki Mochizuki, Rei Ogawa, Shigetomo Fukuhara

Published in: Angiogenesis | Issue 2/2019

Abstract

Angiogenesis, the growth of new blood vessels from pre-existing vessels, is critical for cutaneous wound healing. However, it remains elusive how endothelial cells (ECs) and pericytes (PCs) establish new blood vessels during cutaneous angiogenesis. We set up a live-imaging system to analyze cutaneous angiogenesis in adult zebrafish. First, we characterized basic structures of cutaneous vasculature. In normal skin tissues, ECs and PCs remained dormant to maintain quiescent blood vessels, whereas cutaneous injury immediately induced angiogenesis through the vascular endothelial growth factor signaling pathway. Tortuous and disorganized vessel networks formed within a few weeks after the injury and subsequently normalized through vessel regression in a few months. Analyses of the repair process of injured single blood vessels revealed that severed vessels elongated upon injury and anastomosed with each other. Thereafter, repaired vessels and adjacent uninjured vessels became tortuous by increasing the number of ECs. In parallel, PCs divided and migrated to cover the tortuous blood vessels. ECs sprouted from the PC-covered tortuous vessels, suggesting that EC sprouting does not require PC detachment from the vessel wall. Thus, live imaging of cutaneous angiogenesis in adult zebrafish enables us to clarify how ECs and PCs develop new blood vessels during cutaneous angiogenesis.

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Title: Live imaging of angiogenesis during cutaneous wound healing in adult zebrafish
Authors: Chikage Noishiki
Shinya Yuge
Koji Ando
Yuki Wakayama
Naoki Mochizuki
Rei Ogawa
Shigetomo Fukuhara
Publication date: 01-05-2019
Publisher: Springer Netherlands
Published in: Angiogenesis / Issue 2/2019
Print ISSN: 0969-6970
Electronic ISSN: 1573-7209
DOI: https://doi.org/10.1007/s10456-018-09660-y

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