Top

Esophagus

Published in:

01-07-2020 | Original Article

LncRNA-IUR up-regulates PTEN by sponging miR-21 to regulate cancer cell proliferation and apoptosis in esophageal squamous cell carcinoma

Authors: Bin Wang, Peiyan Hua, Li Zhang, Jindong Li, Yan Zhang

Published in: Esophagus | Issue 3/2020

Abstract

Backgrounds

Imatinib-up-regulated lncRNA (lncRNA-IUR) is a recently identified tumor suppressor, while its role in ESCC is unknown. This study aimed to investigate the role of lncRNA-IUR in esophageal squamous cell carcinoma (ESCC).

Methods

Between April 2011 and April 2014, the Second Hospital of Jilin University admitted a total of 98 ESCC patients, from whom the present study selected 50 cases (33 males and 17 females; 43–67 years; 54.8 ± 6.0 year). ESCC cells and transient transfections, qPCR, RNA interaction analysis, western blot, CCK-8 assay, and cell apoptosis analysis were applied.

Results

We found that IUR was down-regulated in ESCC, and low levels of IUR predicted poor survival of ESCC patients. In ESCC cells, IUR expression levels were significantly and positively correlated with PTEN mRNA. Bioinformatics analysis showed that IUR may sponge miR-21, which can target PTEN. In ESCC cells, IUR over-expression led to the increased expression levels of PTEN; while miR-21 over-expression led to decreased expression levels of PTEN. IUR and miR-21 failed to affect each other. Cell proliferation and apoptosis analysis showed that IUR and PTEN over-expression inhibited cancer cell proliferation and promoted apoptosis; while miR-21 over-expression played an opposite role. In addition, miR-21 over-expression attenuated the effects of IUR over-expression on PTEN expression and cell proliferation.

Conclusion

Therefore, IUR may up-regulate PTEN by sponging miR-21 to regulate cancer cell proliferation and apoptosis in ESCC.

Lin DC, Wang MR, Koeffler HP. Genomic and epigenomic aberrations in esophageal squamous cell carcinoma and implications for patients. Gastroenterology. 2018;154(2):374–89. https://doi.org/10.1053/j.gastro.2017.06.066.CrossRefPubMed

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin. 2019;69(1):7–34. https://doi.org/10.3322/caac.21551.CrossRefPubMed

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016;66(1):7–30. https://doi.org/10.3322/caac.21332.CrossRefPubMed

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):115–32. https://doi.org/10.3322/caac.21338.CrossRefPubMed

Kashima H, Noma K, Ohara T, Kato T, Katsura Y, Komoto S, Sato H, Katsube R, Ninomiya T, Tazawa H, Shirakawa Y, Fujiwara T. Cancer-associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma. Int J Cancer. 2019;144(4):828–40. https://doi.org/10.1002/ijc.31953.CrossRefPubMed

Muto M, Minashi K, Yano T, Saito Y, Oda I, Nonaka S, Omori T, Sugiura H, Goda K, Kaise M, Inoue H, Ishikawa H, Ochiai A, Shimoda T, Watanabe H, Tajiri H, Saito D. Early detection of superficial squamous cell carcinoma in the head and neck region and esophagus by narrow band imaging: a multicenter randomized controlled trial. J Clin Oncol. 2010;28(9):1566–72. https://doi.org/10.1200/JCO.2009.25.4680.CrossRefPubMedPubMedCentral

Gao YB, Chen ZL, Li JG, Hu XD, Shi XJ, Sun ZM, Zhang F, Zhao ZR, Li ZT, Liu ZY, Zhao YD, Sun J, Zhou CC, Yao R, Wang SY, Wang P, Sun N, Zhang BH, Dong JS, Yu Y, Luo M, Feng XL, Shi SS, Zhou F, Tan FW, Qiu B, Li N, Shao K, Zhang LJ, Zhang LJ, Xue Q, Gao SG, He J. Genetic landscape of esophageal squamous cell carcinoma. Nat Genet. 2014;46(10):1097–102. https://doi.org/10.1038/ng.3076.CrossRefPubMed

Lin DC, Hao JJ, Nagata Y, Xu L, Shang L, Meng X, Sato Y, Okuno Y, Varela AM, Ding LW, Garg M, Liu LZ, Yang H, Yin D, Shi ZZ, Jiang YY, Gu WY, Gong T, Zhang Y, Xu X, Kalid O, Shacham S, Ogawa S, Wang MR, Koeffler HP. Genomic and molecular characterization of esophageal squamous cell carcinoma. Nat Genet. 2014;46(5):467–73. https://doi.org/10.1038/ng.2935.CrossRefPubMedPubMedCentral

Georgescu MM. PTEN tumor suppressor network in PI3K-Akt pathway control. Genes Cancer. 2010;1(12):1170–7. https://doi.org/10.1177/1947601911407325.CrossRefPubMedPubMedCentral

10.

Li H, Gao Q, Guo L, Lu SH. The PTEN/PI3K/Akt pathway regulates stem-like cells in primary esophageal carcinoma cells. Cancer Biol Ther. 2011;11(11):950–8. https://doi.org/10.4161/cbt.11.11.15531.CrossRefPubMed

11.

Zhang JG, Wang JJ, Zhao F, Liu Q, Jiang K, Yang GH. MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC). Clin Chim Acta. 2010;411(11–12):846–52. https://doi.org/10.1016/j.cca.2010.02.074.CrossRefPubMed

12.

Paraskevopoulou MD, Hatzigeorgiou AG. Analyzing MiRNA–LncRNA interactions. Methods Mol Biol. 2016;1402:271–86. https://doi.org/10.1007/978-1-4939-3378-5_21.CrossRefPubMed

13.

Wang X, Yang J, Guo G, Feng R, Chen K, Liao Y, Zhang L, Sun L, Huang S, Chen JL. Novel lncRNA-IUR suppresses Bcr-Abl-induced tumorigenesis through regulation of STAT5-CD71 pathway. Mol Cancer. 2019;18(1):84. https://doi.org/10.1186/s12943-019-1013-3.CrossRefPubMedPubMedCentral

14.

Hueman MT, Wang H, Yang CQ, Sheng L, Henson DE, Schwartz AM, Chen D. Creating prognostic systems for cancer patients: a demonstration using breast cancer. Cancer Med. 2018;7(8):3611–21. https://doi.org/10.1002/cam4.1629.CrossRefPubMedPubMedCentral

15.

Pfeffer SR, Yang CH, Pfeffer LM. The role of miR-21 in cancer. Drug Dev Res. 2015;76(6):270–7. https://doi.org/10.1002/ddr.21257.CrossRefPubMed

16.

Yang Y, Guo JX, Shao ZQ. miR-21 targets and inhibits tumor suppressor gene PTEN to promote prostate cancer cell proliferation and invasion: an experimental study. Asian Pac J Trop Med. 2017;10(1):87–91. https://doi.org/10.1016/j.apjtm.2016.09.011.CrossRefPubMed

17.

Wu XS, Wang F, Li HF, Hu YP, Jiang L, Zhang F, Li ML, Wang XA, Jin YP, Zhang YJ, Lu W, Wu WG, Shu YJ, Weng H, Cao Y, Bao RF, Liang HB, Wang Z, Zhang YC, Gong W, Zheng L, Sun SH, Liu YB. LncRNA-PAGBC acts as a microRNA sponge and promotes gallbladder tumorigenesis. EMBO Rep. 2017;18(10):1837–53. https://doi.org/10.15252/embr.201744147.CrossRefPubMedPubMedCentral

Title: LncRNA-IUR up-regulates PTEN by sponging miR-21 to regulate cancer cell proliferation and apoptosis in esophageal squamous cell carcinoma
Authors: Bin Wang
Peiyan Hua
Li Zhang
Jindong Li
Yan Zhang
Publication date: 01-07-2020
Publisher: Springer Singapore
Published in: Esophagus / Issue 3/2020
Print ISSN: 1612-9059
Electronic ISSN: 1612-9067
DOI: https://doi.org/10.1007/s10388-020-00724-x

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

LncRNA-IUR up-regulates PTEN by sponging miR-21 to regulate cancer cell proliferation and apoptosis in esophageal squamous cell carcinoma

Abstract

Backgrounds

Methods

Results

Conclusion

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Backgrounds

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 3/2020

Clinical significance of preoperative serum concentrations of interleukin-6 as a prognostic marker in patients with esophageal cancer

Chemoradiotherapy and radiotherapy alone in patients with esophageal cancer aged 80 years or older based on the Comprehensive Registry of Esophageal Cancer in Japan

Predictors of reflux aspiration and laryngo-pharyngeal reflux

Therapeutic results of proton beam therapy with concurrent chemotherapy for cT1 esophageal cancer and salvage endoscopic therapy for local recurrence

Anomalies of the right vertebral vein increasing the difficulty of lymph-node dissection along the right recurrent laryngeal nerve: a single-institution, retrospective study

The sterno-tracheal distance is an important factor of anastomotic leakage of retrosternal gastric tube reconstruction after esophagectomy

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page