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Clinical and Experimental Medicine
Published in: Clinical and Experimental Medicine 4/2017

01-11-2017 | Original Article

Spatial distribution of mast cells around vessels and glands in human gastric carcinoma

Authors: Diego Guidolin, Simona Ruggieri, Tiziana Annese, Cinzia Tortorella, Andrea Marzullo, Domenico Ribatti

Published in: Clinical and Experimental Medicine | Issue 4/2017

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Abstract

The spatial distribution of mast cells inside the tumor stroma has been little investigated. In this study, we have evaluated tumor mast cells (MCs) distribution in gastric cancer through the analysis of the morphological features of the spatial patterns generated by these cells, including size, shape, and architecture of the cell pattern. The pattern of distribution of tryptase- and chymase-positive MCs around the blood vessels and gastric glands in human gastric adenocarcinoma samples was investigated by immunohistochemical techniques and by introducing a quantitative approach to characterize the spatial distribution of MCs. In human gastric cancer, both chymase-positive MC and vessels exhibited significant deviations from randomness for what it concerns their spatial relationship with gastric parenchyma. As indicated by cell-to-gland distances shorter than expected by chance, in grade II samples a preferential localization of chymase-positive MC near the gastric glands was observed. Interestingly, the same type of spatial association was exhibited by vessels in grade IV samples, where vessel-to-gland distances shorter than expected by chance were observed. These two findings allow to speculate about a sequence of events in which a subpopulation of MC is first recruited around gastric parenchyma to drive the subsequent development of a vascular support to the tissue.
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Metadata
Title
Spatial distribution of mast cells around vessels and glands in human gastric carcinoma
Authors
Diego Guidolin
Simona Ruggieri
Tiziana Annese
Cinzia Tortorella
Andrea Marzullo
Domenico Ribatti
Publication date
01-11-2017
Publisher
Springer International Publishing
Published in
Clinical and Experimental Medicine / Issue 4/2017
Print ISSN: 1591-8890
Electronic ISSN: 1591-9528
DOI
https://doi.org/10.1007/s10238-017-0452-7

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