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Clinical and Experimental Nephrology
Published in: Clinical and Experimental Nephrology 5/2015

01-10-2015 | Original Article

pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney

Authors: Yukiko Yasuoka, Yuichi Sato, Jillian M. Healy, Hiroshi Nonoguchi, Katsumasa Kawahara

Published in: Clinical and Experimental Nephrology | Issue 5/2015

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Abstract

Background

The localization and role of the calcium-sensing receptor (CaSR) along the nephron including the collecting ducts is still open to debate.

Methods

Using the quantitative, highly sensitive in situ hybridization technique and a double-staining immunohistochemistry technique, we investigated the axial distribution and expression of CaSR along the nephron in mice (C57B/6J) treated for 6 days with acid or alkali diets.

Results

Under control condition, CaSR was specifically localized in the cortical and medullary thick ascending limb of Henle’s loop (CTAL and MTAL), macula densa (MD), distal convoluted tubule (DCT), and CCD (TALs, MD > DCT, CCD). Along the CCD, CaSR was co-localized with an anion exchanger type 4 (AE4), a marker of the basolateral membrane of type-B intercalated cell (IC-B) in mice. On the contrary, CaSR was not detected either in principal cells (PC) or in type-A intercalated cell (IC-A). CaSR expression levels in IC-B significantly (P < 0.005) decreased when mice were fed NH4Cl (acid) diets and increased when animals were given NaHCO3 (alkali) diets. As expected, cell heights of IC-A and IC-B significantly (P < 0.005) increased in the above experimental conditions. Surprisingly, single infusion (ip) of neomycin, an agonist of CaSR, significantly (P < 0.005) increased urinary Ca excretion without further increasing the hourly urine volume and significantly (P < 0.05) decreased urine pH.

Conclusion

CaSR, cloned from rat kidney, was localized in the basolateral membrane of IC-B and was more expressed during alkali-loading. Its alkali-sensitive expression may promote urinary alkali secretion for body acid–base balance.
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Metadata
Title
pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney
Authors
Yukiko Yasuoka
Yuichi Sato
Jillian M. Healy
Hiroshi Nonoguchi
Katsumasa Kawahara
Publication date
01-10-2015
Publisher
Springer Japan
Published in
Clinical and Experimental Nephrology / Issue 5/2015
Print ISSN: 1342-1751
Electronic ISSN: 1437-7799
DOI
https://doi.org/10.1007/s10157-014-1063-1

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