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01-04-2022 | Sarcopenia | Original Article

Photobiomodulation intervention improves oxidative, inflammatory, and morphological parameters of skeletal muscle in cirrhotic Wistar rats

Authors: Diogo Scalon, Jaqueline Nascimento Picada, Jayne Torres de Sousa, Ariane Tainá da Silva, Josieli Raskopf Colares, Norma Anair Possa Marroni

Published in: Lasers in Medical Science | Issue 3/2022

Abstract

Photobiomodulation (PBM) might be an intervention method to mitigate sarcopenia in cirrhotic patients. Given the lack of research on this issue, the goal of this study was to evaluate possible beneficial effects of PBM on the structural and functional properties of skeletal muscle from cirrhotic rats. Cirrhosis was induced by secondary bile duct ligation (BDL). Wistar rats were randomized into four groups: sham-operated control (Sham), Sham + PBM, BDL, and BDL + PBM. After cirrhosis induction, a dose of PBM (1 J; 100mW; 10 s; 880 nm; 6 × per week) was applied to each quadriceps, from the 15th to the 45th day after surgery. The locomotor ability was performed using an open-field task. The muscle structure was analyzed using histological methods. Cell damage was also evaluated assessing oxidative stress and DNA damage markers, and IL-1β pro-inflammatory interleukin by immunohistochemical analysis. An increase in the number of crossings was observed in the BDL + PBM group in relation to BDL. The BDL group showed muscle atrophy and increased IL-1β in relation to Sham, while in the BDL + PBM group, the fiber muscle was restructured and there was a decrease of IL-1 β. TBARS increased in the liver and muscle tissues in the BDL group and decreased it in the BDL + PBM group. SOD increased while CAT decreased in the BDL + PBM group in relation to the BDL group. No genotoxic or mutagenic effect was observed for PBM treatment. PBM improved the locomotion and the morphology of the muscle fibers, decreasing oxidative stress and inflammation, without causing DNA damage in cirrhotic rats.

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Title: Photobiomodulation intervention improves oxidative, inflammatory, and morphological parameters of skeletal muscle in cirrhotic Wistar rats
Authors: Diogo Scalon
Jaqueline Nascimento Picada
Jayne Torres de Sousa
Ariane Tainá da Silva
Josieli Raskopf Colares
Norma Anair Possa Marroni
Publication date: 01-04-2022
Publisher: Springer London
Keyword: Sarcopenia
Published in: Lasers in Medical Science / Issue 3/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03458-z

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Photobiomodulation intervention improves oxidative, inflammatory, and morphological parameters of skeletal muscle in cirrhotic Wistar rats

Abstract

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Abstract

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