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Lasers in Medical Science
Published in: Lasers in Medical Science 2/2022

01-03-2022 | Photodynamic Therapy | Original Article

Efficacy of 5-aminolevulinic acid-based photodynamic therapy in different subtypes of canine mammary gland cancer cells

Authors: Ozge Turna, Aslihan Baykal, Elif Sozen Kucukkara, Ozge Ozten, Asuman Deveci Ozkan, Gamze Guney Eskiler, Ali Furkan Kamanli, Cemil Bilir, Salih Zeki Yildiz, Suleyman Kaleli, Melih Ucmak, Guven Kasikci, Hyun Soo Lim

Published in: Lasers in Medical Science | Issue 2/2022

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Abstract

Canine mammary gland tumors (CMGTs) are heterogeneous disease and subclassified [sarcomas (S), carcinomas (C), and carcinosarcomas (CS)] according to histopathological differentiation. Photodynamic therapy (PDT) is a promising treatment strategy based on the use of a photosensitizer (PS) activated by light. However, the therapeutic potential of PDT in the treatment of CMGTs has not been investigated, yet. Therefore, the aim of this study was to determine the in vitro protocol of 5-ALA-based-PDT for the treatment of three subtypes of CMGTs, for the first time. The intracellular PpIX florescence intensity was measured for 5-ALA (0.5 and 1 mM). After irradiation with different light doses (6, 9, 12, 18, and 24 J/cm2) for two different modes [continuous wave (CW) and pulse radiation (PR)], the cytotoxic effects of 5-ALA (0.5 and 1 mM) on the subtypes (C, S, and CS) of CMGTs were analyzed by WST-1. Finally, the optimal PDT treatment protocol was validated through Annexin V and AO/EtBr staining. Our results showed that 1 mM 5-ALA for 4-h incubation was the best treatment condition in all subtypes of CMGTs due to higher intracellular PpIX level. After irradiation with different light doses, PR mode was more effective in S primary cells at 9 J/cm2. However, a significant decrease in the viability of C and CS cells was detected at 12 /cm2 in CW mode (p < 0.05). Additionally, 1 mM 5-ALA induced apoptotic cell death in each subtype of CMGTs. Our preliminary findings suggest that (i) each subtype of CMGTs differentially responds to PDT and (ii) the light dose and mode could play an important role in the effective PDT treatment. However, further studies are needed to investigate the role of the different light sources and PDT-based apoptotic cell death in CMGTs cells.
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Metadata
Title
Efficacy of 5-aminolevulinic acid-based photodynamic therapy in different subtypes of canine mammary gland cancer cells
Authors
Ozge Turna
Aslihan Baykal
Elif Sozen Kucukkara
Ozge Ozten
Asuman Deveci Ozkan
Gamze Guney Eskiler
Ali Furkan Kamanli
Cemil Bilir
Salih Zeki Yildiz
Suleyman Kaleli
Melih Ucmak
Guven Kasikci
Hyun Soo Lim
Publication date
01-03-2022
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 2/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-021-03324-y

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