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Lasers in Medical Science
Published in: Lasers in Medical Science 3/2021

01-04-2021 | Arthritis | Original Article

Laser treatment of synovial inflammatory process in experimentally induced microcrystalline arthritis in Wistar rats

Authors: Débora Mara Fortes Bartoli, Airton Luiz Felizatti, Fernando Russo Costa do Bomfim, Julia Leme Bovo, Andrea Aparecida de Aro, Maria Esméria Corezzola do Amaral, Marcelo Augusto Marretto Esquisatto

Published in: Lasers in Medical Science | Issue 3/2021

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Abstract

The presence of intra-articular crystals is detected in different articular pathologies of acute or chronic nature. The aim of this work was to analyze the action of the indium gallium aluminum and phosphorus (InGaAlP) (λ = 670 nm) laser on the synovial membrane present in the knee joint in experimentally induced microcrystalline arthritis in male adult Wistar rats. The animals were divided into three experimental groups (n = 24): control (A), experimentally induced arthritis (B), experimentally induced arthritis+InGaAlP laser therapy (C). The laser treatment was made daily in the patellar region of the right knee after 48 h of the experimental induction. After 7, 14, and 21 days of therapy, the rats were euthanized and the right knees were removed and processed for histomorphometric, immunohistochemical, ultrastructural, and biochemical investigation of the synovium. The number of granulocytes on the 14th and 21st days was higher in B and lower in C and, lastly, in A. The number of fibroblasts on the 14th and 21st days was similar between A and C and below B. The number of blood vessels on the 21st day was higher in B than in the other groups. The positive number of cells for the TUNEL test was higher on the 14th and 21st days in B compared to the others. The percentage of tissue area occupied by birefringent collagen fibers was higher in B on the 21st day than in the others. The ultrastructure of cells showed fibroblast-like morphology in all groups and periods evaluated. The quantification of glycosaminoglycans did not present significant differences between the groups in all the experimental periods. The amount of hydroxyproline was higher in B compared to the other groups on the 14th and 21st days. The content of non-collagen proteins was higher in B on the 21st day in relation to the other groups. Quantification of TNF-α on the 21st day was higher in A and B than in C. For TGF-β on the 21st day, groups B and C presented similar and higher values than A. For MMP-13, groups A and B presented data similar to and above C. In relation to ADAMT-S4, on the 21st day, groups B and C presented data similar to and lower than A. InGaAlP-670 nm therapy reduced the inflammatory process and tissue injuries of the synovial membrane in comparison to the untreated group, indicating its potential utilization in clinical studies aiming in the recovery of acute arthritis in patients.
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Metadata
Title
Laser treatment of synovial inflammatory process in experimentally induced microcrystalline arthritis in Wistar rats
Authors
Débora Mara Fortes Bartoli
Airton Luiz Felizatti
Fernando Russo Costa do Bomfim
Julia Leme Bovo
Andrea Aparecida de Aro
Maria Esméria Corezzola do Amaral
Marcelo Augusto Marretto Esquisatto
Publication date
01-04-2021
Publisher
Springer London
Keywords
Arthritis
Laser
Published in
Lasers in Medical Science / Issue 3/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03055-6

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