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01-09-2020 | Caries | Original Article

Monitoring enamel caries on resin-treated occlusal surfaces using quantitative light-induced fluorescence: an in vitro study

Authors: Florense Gabriela Silva, Patricia Moreira Freitas, Fausto Medeiros Mendes, Tatiane Fernandes de Novaes, Michele Baffi Diniz, Renata Oliveira Guaré

Published in: Lasers in Medical Science | Issue 7/2020

Abstract

The aim of this study is to evaluate the ability of quantitative light-induced fluorescence (QLF) to monitor enamel caries lesions of different severity stages located on the occlusal surfaces of permanent teeth before and after treatment with resin infiltrant. Sixty extracted permanent teeth had one occlusal site selected and were categorized according to the International Caries Detection and Assessment System (ICDAS) criteria. The teeth were divided into three groups (n = 20): ICDAS 1, ICDAS 2, and ICDAS 3. The teeth were assessed by a trained examiner using QLF in two phases: (A) before and (B) after treatment with resin infiltrant. The caries lesions were evaluated using the following QLF parameters: area (mm²); ΔF, fluorescence loss (%); and ΔQ, fluorescence loss integrated over the lesion area (%*mm²). The resin infiltrant (Icon™) was applied on the occlusal surface following the manufacturer’s recommendations. The teeth were then sectioned and prepared for polarized light microscopy analysis. The penetration of resin infiltrant was measured with ImageJ. The groups showed a statistically significant difference in all QLF parameters before and after caries infiltration, with the reduction of fluorescence values posttreatment (p < 0.05). Infiltrant penetration was observed in all groups, with a statistical difference between all groups (p < 0.05). The reduction in QLF parameters after resin infiltration suggests that QLF is able to monitor enamel caries lesions of different severity stages located on the occlusal surfaces of permanent teeth before and after treatment with resin infiltrant.

Frencken JE, Sharma P, Stenhouse L, Green D, Laverty D, Dietrich T (2017) Global epidemiology of dental caries and severe periodontitis - a comprehensive review. J Clin Periodontol 44(Suppl 18):S94–S105PubMed

Gimenez T, Piovesan C, Braga MM, Raggio DP, Deery C, Ricketts DN, Ekstrand KR, Mendes FM (2015) Visual inspection for caries detection: a systematic review and meta-analysis. J Dent Res 94(7):895–904PubMed

Ekstrand KR, Gimenez T, Ferreira FR, Mendes FM, Braga MM (2018) The international caries detection and assessment system - ICDAS: a systematic review. Caries Res 52(5):406–419PubMed

Tranæus S, Heinrich-Weltzien R, Kühnisch J, Stösser L, Angmar-Mansson B (2001) Potential applications and limitations of quantitative light-induced fluorescence in dentistry. Med Laser Appl 16:195–204

Heinrich-Weltzien R, Kuhnisch J, van der Veen M, de Josselin de Jong E, Stosser L (2003) Quantitative light-induced fluorescence (QLF)-a potential method for the dental practitioner. Quintessence Int 34(3):181–188PubMed

Pretty IA, Ellwood RP (2013) The caries continuum: opportunities to detect, treat and monitor the re-mineralization of early caries lesions. J Dent 41(2):S12–S21PubMed

Alammari MR, Smith PW, de Josselin de Jong E, Higham SM (2013) Quantitative light-induced fluorescence (QLF): a tool for early occlusal dental caries detection and supporting decision making in vivo. J Dent 41(2):127–132PubMed

Gomez J, Zakian C, Salsone S, Pinto SC, Taylor A, Pretty IA et al (2013) In vitro performance of different methods in detecting occlusal caries lesions. J Dent 41(2):180–186PubMed

Fontana M, Platt JA, Eckert GJ, González-Cabezas C, Yoder K, Zero DT et al (2014) Monitoring of sound and carious surfaces under sealants over 44 months. J Dent Res 93(11):1070–1075PubMedPubMedCentral

10.

Jallad M, Zero D, Eckert G, Ferreira Zandona A (2015) In vitro detection of occlusal caries on permanent teeth by a visual, light-induced fluorescence and photothermal radiometry and modulated luminescence methods. Caries Res 49(5):523–530PubMed

11.

Novaes TF, Reyes A, Matos R, Antunes-Pontes LR, Marques RP, Braga MM et al (2017) Association between quantitative measures obtained using fluorescence-based methods and activity status of occlusal caries lesions in primary molars. Int J Paediatr Dent 27(3):154–162PubMed

12.

Jung EH, Lee ES, Jung HI, Kang SM, de Josselin de Jong E, Kim BI (2018) Development of a fluorescence-image scoring system for assessing noncavitated occlusal caries. Photodiagn Photodyn Ther 21:36–42

13.

Diniz MB, Campos PH, Wilde S, Cordeiro RCL, Zandona AGF (2019) Performance of light-emitting diode device in detecting occlusal caries in the primary molars. Lasers Med Sci 34(6):1235–1241PubMed

14.

Lee HS, Kim SK, Park SW, de Josselin de Jong E, Kwon HK, Jeong SH, Kim BI (2018) Caries detection and quantification around stained pits and fissures in occlusal tooth surfaces with fluorescence. J Biomed Opt 23(9):1–7PubMed

15.

Ferreira Zandoná A, Santiago E, Eckert G, Fontana M, Ando M, Zero DT (2010) Use of ICDAS combined with quantitative light-induced fluorescence as a caries detection method. Caries Res 44(3):317–322PubMedPubMedCentral

16.

Ferreira Zandoná A, Santiago E, Eckert GJ, Katz BP, Pereira de Oliveira S, Capin OR et al (2012) The natural history of dental caries lesions: a 4-year observational study. J Dent Res 91(9):841–846PubMedPubMedCentral

17.

Ferreira Zandoná A, Ando M, Gomez GF, Garcia-Corretjer M, Eckert GJ, Santiago E, Katz BP, Zero DT (2013) Longitudinal analyses of early lesions by fluorescence: an observational study. J Dent Res 92(7):84S–89SPubMed

18.

Yap J, Walsh LJ, Naser-Ud Din S, Ngo H, Manton DJ (2014) Evaluation of a novel approach in the prevention of white spot lesions around orthodontic brackets. Aust Dent J 59(1):70–80PubMed

19.

Yuan H, Li J, Chen L, Cheng L, Cannon RD, Mei L (2014) Esthetic comparison of white-spot lesion treatment modalities using spectrometry and fluorescence. Angle Orthod 84(2):343–349PubMed

20.

Min JH, Inaba D, Kim BI (2016) Evaluation of resin infiltration using quantitative light-induced fluorescence technology. Photodiagn Photodyn Ther 15:6–10

21.

Frencken JE, Peters MC, Manton DJ, Leal SC, Gordan VV, Eden E (2012) Minimal intervention dentistry for managing dental caries - a review: report of a FDI task group. Int Dent J 62(5):223–243PubMedPubMedCentral

22.

Carvalho JC, Dige I, Machiulskiene V, Qvist V, Bakhshandeh A, Fatturi-Parolo C, Maltz M (2016) Occlusal caries: biological approach for its diagnosis and management. Caries Res 50(6):527–542PubMed

23.

Meyer-Lueckel H, Paris S (2008) Improved resin infiltration of natural caries lesions. J Dent Res 87(12):1112–1116PubMed

24.

Abbas BA, Marzouk ES, Zaher AR (2018) Treatment of various degrees of white spot lesions using resin infiltration-in vitro study. Prog Orthod 19(1):27PubMedPubMedCentral

25.

Bakhshandeh A, Ekstrand K (2015) Infiltration and sealing versus fluoride treatment of occlusal caries lesions in primary molar teeth. 2-3 years results. Int J Paediatr Dent 25(1):43–50PubMed

26.

Lausch J, Paris S, Selje T, Dörfer CE, Meyer-Lueckel H (2015) Resin infiltration of fissure caries with various techniques of pretreatment in vitro. Caries Res 49(1):50–55PubMed

27.

Anauate-Netto C, Neto BL, Amore R, Di Hipólito V, D’Alpino PHP (2017) Caries progression in non-cavitated fissures after infiltrant application: a 3-year follow-up of a randomized controlled clinical trial. J Appl Oral Sci 25(4):442–454PubMedPubMedCentral

28.

Al Tuwirqi AA, Alshammari AM, Felemban OM, Ali Farsi NM (2019) Comparison of penetration depth and microleakage of resin infiltrant and conventional sealant in pits and fissures of permanent teeth in vitro. J Contemp Dent Pract 20(11):1339–1344PubMed

29.

Kielbassa AM, Ulrich I, Schmidl R, Schüller C, Frank W, Werth VD (2017) Resin infiltration of deproteinised natural occlusal subsurface lesions improves initial quality of fissure sealing. Int J Oral Sci 9(2):117–124PubMedPubMedCentral

30.

Lausch J, Askar H, Paris S, Meyer-Lueckel H (2017) Micro-filled resin infiltration of fissure caries lesions in vitro. J Dent 57:73–76PubMed

31.

Schneider H, Park KJ, Rueger C, Ziebolz D, Krause F, Haak R (2017) Imaging resin infiltration into non-cavitated carious lesions by optical coherence tomography. J Dent 60:94–98PubMed

32.

Paris S, Lausch J, Selje T, Dörfer CE, Meyer-Lueckel H (2014) Comparison of sealant and infiltrant penetration into pit and fissure caries lesions in vitro. J Dent 42(4):432–438PubMed

33.

Faul F, Erdfelder E, Lang AG, Buchner A (2007) G*power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39(2):175–191PubMed

34.

Ismail AI, Sohn W, Tellez M, Amaya A, Sen A, Hasson H et al (2007) The international caries detection and assessment system (ICDAS): an integrated system for measuring dental caries. Community Dent Oral Epidemiol 35(3):170–178PubMed

35.

Francescut P, Zimmerli B, Lussi A (2006) Influence of different storage methods on laser fluorescence values: a two-year study. Caries Res 40(3):181–185PubMed

36.

Swamy DF, Barretto ES, Mallikarjun SB, Dessai SSR (2017) In vitro evaluation of resin infiltrant penetration into white spot lesions of deciduous molars. J Clin Diagn Res 11(9):ZC71–ZC74PubMedPubMedCentral

37.

Van der Veen MH, de Josselin de Jong E (2000) Application of quantitative lightinduced fluorescence for assessing early caries lesions. Monogr Oral Sci 17:144–162PubMed

38.

De Josselin de Jong ED, Higham SM, Smith PW, van Daelen CJ, van der Veen MH (2009) Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease. J Appl Phys 105(10):102031

39.

Deery C, Iloya J, Nugent ZJ, Srinivasan V (2006) Effect of placing a clear sealant on the validity and reproducibility of occlusal caries detection by a laser fluorescence device: an in vitro study. Caries Res 40(3):186–193PubMed

40.

Gostanian HV, Shey Z, Kasinathan C, Caceda J, Janal MN (2006) An in vitro evaluation of the effect of sealant characteristics on laser fluorescence for caries detection. Pediatr Dent 28(5):445–450PubMed

41.

Diniz MB, Rodrigues JA, Hug I, Cordeiro RC, Lussi A (2008) The influence of pit and fissure sealants on infrared fluorescence measurements. Caries Res 42(5):328–333PubMed

42.

Kim BR, Kang SM, Kim GM, Kim BI (2016) Differences in the intensity of light-induced fluorescence emitted by resin composites. Photodiagn Photodyn Ther 13:114–119

43.

Ekstrand KR, Ricketts DNJ, Kidd EAM, Qvist V, Schou S (1998) Detection,diagnosing, monitoring and logical treatment of occlusal caries in relation to lesion activity and severity: an in vivo examination with histological validation. Caries Res 32(4):247–254PubMed

44.

Hibst R, Paulus R, Lussi A (2001) Detection of occlusal caries by laser fluorescence: basic and clinical investigations. Med Laser Appl 16:205–213

45.

Ekstrand KR, Martignon S, Ricketts DJ, Qvist V (2007) Detection and activity assessment of primary coronal caries lesions: a methodologic study. Oper Dent 32(3):225–235PubMed

46.

Paris S, Bitter K, Naumann M, Dörfer CE, Meyer-Lueckel H (2011) Resin infiltration of proximal caries lesions differing in ICDAS codes. Eur J Oral Sci 119(2):182–186PubMed

47.

Ando M, Eckert GJ, Stookey GK, Zero DT (2004) Effect of imaging geometry on evaluating natural white-spot lesions using quantitative light-induced fluorescence. Caries Res 38(1):39–44PubMed

48.

Yin W, Feng Y, Hu D, Ellwood R, Pretty I (2007) Reliability of quantitative laser fluorescence analysis of smooth surface lesions adjacent to the gingival tissues. Caries Res 41:186–189PubMed

Title: Monitoring enamel caries on resin-treated occlusal surfaces using quantitative light-induced fluorescence: an in vitro study
Authors: Florense Gabriela Silva
Patricia Moreira Freitas
Fausto Medeiros Mendes
Tatiane Fernandes de Novaes
Michele Baffi Diniz
Renata Oliveira Guaré
Publication date: 01-09-2020
Publisher: Springer London
Keyword: Caries
Published in: Lasers in Medical Science / Issue 7/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03032-z

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Monitoring enamel caries on resin-treated occlusal surfaces using quantitative light-induced fluorescence: an in vitro study

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