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Lasers in Medical Science
Published in: Lasers in Medical Science 2/2020

01-03-2020 | Original Article

Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy

Authors: Letícia Parada Moreira, Débora Dias Ferraretto Moura Rocco, Alexandre Galvão da Silva, Marcos Tadeu Tavares Pacheco, Landulfo Silveira Jr

Published in: Lasers in Medical Science | Issue 2/2020

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Abstract

High-level sport requires analysis of athletes’ metabolic conditions in order to improve the training. Raman spectroscopy can be used to assess urinary composition advantageously when compared to conventional methods of urinalysis. In this work, Raman spectroscopy has been employed to detect creatine in urine of professional swimmers before and after training compared to sedentaries. It has been collected urine samples from five swimmers before and immediately after 150 min of swimming and submitted to Raman spectroscopy (830 nm excitation, 350 mW laser power, 20 s integration time) and compared to the urine from a control group (14 sedentary subjects). The Raman spectra of urine from four swimmers after training showed peaks related to creatine at 829, 915, 1049, and 1397 cm−1, besides peaks referred to urea, creatinine, ketone bodies, and phosphate. A spectral model estimated the concentration of creatine to be from 0.26 to 0.72 g/dL in the urine of these athletes. The presence of this metabolic biomarker in the urine of some swimmers suggests a metabolic profile influenced by the diet, supplementation, individual metabolism, and the self-response to the training. Raman spectroscopy allows a rapid and reliable detection of creatine excreted in the urine of swimming athletes, which may be used to adjust the nutrition/supplementation of each individual as well as the individual response and energy consumption depending on the type and duration of the training.
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Literature
1.
Sahlin K, Harris RC (2011) The creatine kinase reaction: a simple reaction with functional complexity. Amino Acids 40:1363–1367PubMed
2.
Harris R (2011) Creatine in health, medicine and sport: an introduction to a meeting held at Downing College, University of Cambridge, July 2010. Amino Acids 40:1267–1270PubMed
3.
Kreider RB, Jung YP (2011) Invite review: creatine supplementation in exercise, sport, and medicine. J Exerc Nutr Biochem 15:53–69
4.
Kreider RB, Kalman DS, Antonio J et al (2017) International Society of Sports Nutrition position stand: safety and efficacy of creatine supplementation in exercise, sport, and medicine. J Int Soc Sports Nutr 14:1–18PubMedPubMedCentral
5.
Juhn MS, Tarnopolsky M (1998) Oral creatine supplementation and athletic performance: a critical review. Clin J Sports Med 8:286–297
6.
Terjung RL, Clarkson P, Eichner ER et al (2000) The physiological and health effects of oral creatine supplementation. Med Sci Sports Exerc 32:706–717PubMed
7.
Santos RVT, Bassit RA, Caperuto EC et al (2004) The effect of creatine supplementation upon inflammatory and muscle soreness markers after a 30km race. Life Sci 75:1917–1924PubMed
8.
Deminice R, Rosa FT, Franco GS et al (2013) Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition 29:1127–1132PubMed
9.
Kilduff LP, Georgiades E, James N et al (2004) The effects of creatine supplementation on cardiovascular, metabolic, and thermoregulatory responses during exercise in the heat in endurance-trained humans. Int J Sport Nutr Exerc Metab 14:443–460PubMed
10.
Tarnopolsky MA (2010) Caffeine and creatine use in sport. Ann Nutr Metab 57:1–8PubMed
11.
Dou X, Yamaguchi Y, Yamamoto H et al (1996) Quantitative analysis of metabolites in urine using a highly precise, compact near-infrared Raman spectrometer. Vib Spectrosc 13:83–89
12.
Bispo JAM, Vieira EES, Silveira L et al (2013) Correlating the amount of urea, creatinine, and glucose in urine from patients with diabetes mellitus and hypertension with the risk of developing renal lesions by means of Raman spectroscopy and principal component analysis. J Biomed Opt 18:87004PubMed
13.
Saatkamp CJ, Almeida ML, Bispo JAM et al (2016) Quantifying creatinine and urea in human urine through Raman spectroscopy aiming at diagnosis of kidney disease. J Biomed Opt 21:37001PubMed
14.
de Almeida ML, Saatkamp CJ, Fernandes AB et al (2016) Estimating the concentration of urea and creatinine in the human serum of normal and dialysis patients through Raman spectroscopy. Lasers Med Sci 31:1415–1423PubMed
15.
Vieira EES, Bispo JAM, Silveira L, Fernandes AB (2017) Discrimination model applied to urinalysis of patients with diabetes and hypertension aiming at diagnosis of chronic kidney disease by Raman spectroscopy. Lasers Med Sci 32:1605–1613
16.
Hanlon E, Manoharan R, Koo T et al (2000) Prospects for in vivo Raman spectroscopy. Phys Med Biol 45:R1PubMed
17.
Silveira L, Borges RCF, Navarro RS et al (2017) Quantifying glucose and lipid components in human serum by Raman spectroscopy and multivariate statistics. Lasers Med Sci 32:787–795PubMed
18.
Rohleder D, Kocherscheidt G, Gerber K et al (2005) Comparison of mid-infrared and Raman spectroscopy in the quantitative analysis of serum. J Biomed Opt 10:31108
19.
Rupérez A, Montes R, Laserna JJ (1991) Identification of stimulant drugs by surface-enhanced Raman spectrometry on colloidal silver. Vib Spectrosc 2:145–154
20.
Premasiri WR, Clarke RH, Womble ME (2001) Urine analysis by laser Raman spectroscopy. Lasers Surg Med 28:330–334PubMed
21.
Guimarães AE, Pacheco MTT, Silveira L et al (2006) Near infrared Raman spectroscopy (NIRS): a technique for doping control. Spectroscopy 20:185–194
22.
Moreira LP, Silveira L, Pacheco MTT et al (2018) Detecting urine metabolites related to training performance in swimming athletes by means of Raman spectroscopy and principal component analysis. J Photochem Photobiol B Biol 185:223–234
23.
Moreira LP, Silveira L, da Silva AG et al (2017) Raman spectroscopy applied to identify metabolites in urine of physically active subjects. J Photochem Photobiol B Biol 176:92–99
24.
Vandenabeele P (2013) Practical Raman spectroscopy: an introduction. J. Wiley & Sons, Chichester
25.
Ostrovskii DI, Yaremko AM, Vorona IP (1997) Nature of background scattering in Raman spectra of materials containing high-wavenumber vibrations. J Raman Spectrosc 28:771–778
26.
Lieber CA, Mahadevan-Jansen A (2003) Automated method for subtraction of fluorescence from biological Raman spectra. Appl Spectrosc 57:1363–1367PubMed
27.
Bell SEJ, Stewart SP, Speers SJ (2012) Infrared and Raman spectroscopy in forensic science. John Wiley & Sons, Chichester
28.
Silveira FL, Pacheco MTT, Bodanese B et al (2015) Discrimination of non-melanoma skin lesions from non-tumor human skin tissues in vivo using Raman spectroscopy and multivariate statistics. Lasers Surg Med 47:6–16PubMed
29.
Jolliffe IT (1995) Principal components analysis. Springer-Velag, New York
30.
Keuleers R, Desseyn HO, Rousseau B et al (1999) Vibrational analysis of urea. J Phys Chem A 103:4621–4630
31.
Bayrak C, Bayarı SH (2010) Vibrational and DFT studies of creatinine and its metal complexes. J Biol Chem 38:107–188
32.
Podstawka E, Światłowska M, Borowiec E et al (2007) Food additives characterization by infrared, Raman, and surface-enhanced Raman spectroscopies. J Raman Spectrosc 38:356–363
33.
Socrates G (2004) Infrared and Raman characteristic group frequencies - tables and charts. J. Wiley & Sons, Chichester
34.
Lambert JB, Shurvell HF, Cooks RG (1987) Introduction to organic spectroscopy. Macmillan, New York
35.
De Gelder J, Willemse-Erix D, Scholtes MJ et al (2008) Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy. Anal Chem 80:2155–2160PubMed
36.
Furukawa T, Sato H, Murakami R et al (2006) Raman microspectroscopy study of structure, dispersibility, and crystallinity of poly (hydroxybutyrate)/poly (l-lactic acid) blends. Polymer 47:3132–3140
37.
Hoccart X, Turrell G (1993) Raman spectroscopic investigation of the dynamics of urea–water complexes. J Chem Phys 99:8498–8503
38.
Frost RL, Kristof J, Rintoul L et al (2000) Raman spectroscopy of urea and urea-intercalated kaolinites at 77 K. Spectrochim Acta A Mol Biomol Spectrosc 56:1681–1691
39.
McMurdy JW, Berger AJ (2003) Raman spectroscopy-based creatinine measurement in urine samples from a multipatient population. Appl Spectrosc 57:522–525PubMed
40.
41.
42.
Mossoba MM (1998) Spectral methods in food analysis: instrumentation and applications. Marcel Dekker, New York
43.
44.
Wyss M, Kaddurah-Daouk R (2000) Creatine and creatinine metabolism. Physiol Rev 80:1107–1213PubMed
45.
McArdle WD, Katch FI (2009) Exercise physiology: nutrition, energy, and human performance. Lippincott Williams & Wilkins, Philadelphia
46.
47.
Brudnak MA (2004) Creatine: are the benefits worth the risk. Toxicol Lett 150:123–130PubMed
48.
Bezrati-Benayed I, Nasrallah F, Feki M et al (2014) Urinary creatine at rest and after repeated sprints in athletes: a pilot study. Biol Sport 31:49–54PubMedPubMedCentral
49.
De Gelder J, De Gussem K, Vandenabeele P et al (2007) Reference database of Raman spectra of biological molecules. J Raman Spectrosc 38:1133–1147
50.
Tao Z, Peng L, Zhang P et al (2016) Probing the kinetic anabolism of poly-beta-hydroxybutyrate in Cupriavidus necator h16 using single-cell Raman spectroscopy. Sensors 16:1257
51.
52.
Robinson M, Williamson DH (1980) Physiological roles of ketone bodies as substrates and signals in mammalian tissues. Physiol Rev 60:143–187PubMed
53.
Clarke K, Tchabanenko K, Pawlosky R et al (2012) Kinetics, safety and tolerability of (R)-3-hydroxybutyl (R)-3-hydroxybutyrate in healthy adult subjects. Regul Toxicol Pharmacol 63:401–408PubMed
54.
55.
Laffel L (1999) Ketone bodies: a review of physiology, pathophysiology and application of monitoring to diabetes. Diabetes Metab Res Rev 15:412–426PubMed
56.
Newman JC, Verdin E (2014) Ketone bodies as signaling metabolites. Trends Endocrinol Metab 25:42–52PubMed
57.
Cox PJ, Clarke K (2014) Acute nutritional ketosis: implications for exercise performance and metabolism. Extrem Physiol Med 3:17PubMedPubMedCentral
58.
Askew EW, Dohm GL, Huston RL (1975) Fatty acid and ketone body metabolism in the rat: response to diet and exercise. J Nutr 105:1422–1432PubMed
59.
Walsh NP, Blannin AK, Clark AM et al (1998) The effects of high-intensity intermittent exercise on the plasma concentrations of glutamine and organic acids. Eur J Appl Physiol Occup Physiol 77:434–438PubMed
60.
Koo GH, Woo J, Kang S et al (2014) Effects of supplementation with BCAA and L-glutamine on blood fatigue factors and cytokines in juvenile athletes submitted to maximal intensity rowing performance. J Phys Ther Sci 26:1241–1246PubMedPubMedCentral
61.
Beelen M, Burke LM, Gibala MJ et al (2010) Nutritional strategies to promote postexercise recovery. Int J Sport Nutr Exerc Metab 20:515–532PubMed
62.
Bartlett JD, Hawley JA, Morton JP (2015) Carbohydrate availability and exercise training adaptation: too much of a good thing? Eur J Sport Sci 15:3–12PubMed
63.
Burke LM, Hawley JA, Wong SHS et al (2011) Carbohydrates for training and competition. J Sports Sci 29:S17–S27PubMed
64.
Cermak NM, Van Loon LJC (2013) The use of carbohydrates during exercise as an ergogenic aid. Sports Med 43:1139–1155PubMed
65.
Johnson RH, Walton JL, Krebs HA et al (1969) Metabolic fuels during and after severe exercise in athletes and non-athletes. Lancet 2:452–455PubMed
66.
Johnson RH, Walton JL (1971) Fitness, fatness, and post-exercise ketosis. Lancet 297:566–568
67.
Johnson RH, Walton JL (1972) The effect of exercise upon acetoacetate metabolism in athletes and non-athletes. Q J Exp Physiol Cogn Med Sci 57:73–79PubMed
68.
Winder WW, Baldwin KM, Holloszy JO (1975) Exercise-induced increase in the capacity of rat skeletal muscle to oxidize ketones. Can J Physiol Pharmacol 53:86–91PubMed
69.
Balsom PD, Ekblom B, Söerlund K et al (1993) Creatine supplementation and dynamic high-intensity intermittent exercise. Scand J Med Sci Sports 3:143–149
70.
Lindh AM, Peyrebrune MC, Ingham SA et al (2008) Sodium bicarbonate improves swimming performance. Int J Sports Med 29:519–523PubMed
71.
Gant N, Ali A, Foskett A (2010) The influence of caffeine and carbohydrate coingestion on simulated soccer performance. Int J Sport Nutr Exerc Metab 20:191–197PubMed
72.
Bompa TO, Buzzichelli CA (2019) Periodization: theory and methodology of training. Human Kinetics, Champaign
Metadata
Title
Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy
Authors
Letícia Parada Moreira
Débora Dias Ferraretto Moura Rocco
Alexandre Galvão da Silva
Marcos Tadeu Tavares Pacheco
Landulfo Silveira Jr
Publication date
01-03-2020
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 2/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02843-z

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