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01-07-2019 | Original Article

Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Authors: Shigeki Uchinuma, Yasushi Shimada, Khairul Matin, Keiichi Hosaka, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami

Published in: Lasers in Medical Science | Issue 5/2019

Abstract

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD₄₉₀) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD₄₉₀ measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.

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Title: Effects of UVB and UVC irradiation on cariogenic bacteria in vitro
Authors: Shigeki Uchinuma
Yasushi Shimada
Khairul Matin
Keiichi Hosaka
Masahiro Yoshiyama
Yasunori Sumi
Junji Tagami
Publication date: 01-07-2019
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 5/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2685-4

ACC 2024 Congress

Springer Medicine

Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

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