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01-07-2019 | Original Article

The impacts of laser zona thinning on hatching and implantation of vitrified-warmed mouse embryos

Authors: Zhengyuan Huang, Jinghao Liu, Lei Gao, Qingrui Zhuan, Yuxi Luo, Shien Zhu, Kaiyu Lei, Xiangwei Fu

Published in: Lasers in Medical Science | Issue 5/2019

Abstract

Embryo vitrification has advantages in assisted reproduction yet it also induces zona hardening. Laser zona thinning (LZT) is considered as a solution yet its efficacy and security have not been well studied. In this study, we used vitrified-warmed morulae from 2-month-old and 10-month-old ICR female mice as model to investigate the impacts that LZT treatment brings to the in vitro hatching process and implantation by analyzing hatching rate, implantation rate, and blastocyst quality. The results showed that the fully hatched rate was significantly higher after LZT treatment for both young (25.7% vs. 16.2%, P < 0.05) and aged (36.6% vs. 13.2%, P < 0.01) mice. For zona-thinned morulae in young mice, its onset of hatching occurred earlier (28.6% vs. 8.8%, P < 0.01) at D4 and with a greater percentage of U-shaped hatching at D5 (48.3% vs. 33.0%, P < 0.05). LZT treatment did not induce expression change of apoptosis-related genes in all groups (P > 0.05), but for young mice, the total cell number of day 5 blastocyst in zona-thinned group was significantly less than that of the control group (40.6 ± 5.1 vs. 59.9 ± 14.5, P < 0.01). At last, there was an increasing implantation rate in zona-thinned compared to the control group for young (63.8% vs. 52.5%, P > 0.05) and aged (55.6% vs. 47.2%; P > 0.05) mice after embryos were bilaterally transferred in the same recipient. In conclusion, the significant increase of fully hatched rate after LZT treatment is related to the advanced onset of hatching as well as the enhancement of superior hatching structure, and LZT also lead to a better implantation after embryo transfer.

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Trounson A, Mohr L (1983) Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 305:707–709CrossRefPubMed

Chen ZJ, Shi Y, Sun Y, Zhang B, Liang X, Cao Y et al (2016) Fresh versus frozen embryos for infertility in the polycystic ovary syndrome. N Engl J Med 375:523–533CrossRefPubMed

Tucker MJ, Cohen J, Massey JB, Mayer MP, Wiker SR, Wright G (1991) Partial dissection of the zona pellucida of frozen-thawed human embryos may enhance blastocyst hatching, implantation, and pregnancy rates. Am J Obstet Gynecol 165:341–344CrossRefPubMed

Kanyo K, Zeke J, Kriston R, Szucs Z, Cseh S, Somoskoi B et al (2016) The impact of laser-assisted hatching on the outcome of frozen human embryo transfer cycles. Zygote 24:742–747CrossRefPubMed

Moreira DSF, Metelo R (2010) Relation between physical properties of the zona pellucida and viability of bovine embryos after slow-freezing and vitrification. Reprod Domest Anim 40:205–209CrossRef

Tian SJ, Yan CL, Yang HX, Zhou GB, Yang ZQ, Zhu SE (2007) Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes and decrease sperm penetration. Anim Reprod Sci 101:365–371CrossRefPubMed

Cohen J, Alikani M, Reing AM, Ferrara TA, Trowbridge J, Tucker M (1992) Selective assisted hatching of human embryos. Ann Acad Med Singap 21:565–570PubMed

Kilani SS, Cooke S, Kan AK, Chapman MG (2006) Do age and extended culture affect the architecture of the zona pellucida of human oocytes and embryos? Zygote 14:39–44CrossRefPubMed

Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP et al (1990) Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod 5:7–13CrossRefPubMed

10.

Petersen CG, Mauri AL, Baruffi RL, Oliveira JB, Massaro FC, Elder K et al (2005) Implantation failures: success of assisted hatching with quarter-laser zona thinning. Reprod BioMed Online 10:224–229CrossRefPubMed

11.

Palanker D, Ohad S, Lewis A, Simon A, Shenkar J, Penchas S et al (1991) Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med 11:580–586CrossRefPubMed

12.

Schimmel T, Cohen J, Saunders H, Alikani M (2014) Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse. Hum Reprod 29:2670–2679CrossRefPubMedPubMedCentral

13.

Das S, Blake D, Farquhar C, Seif MM (2009) Assisted hatching on assisted conception (IVF and ICSI). Cochrane Database Syst Rev 12:D1894

14.

Cohen J (1991) Assisted hatching of human embryos. J In Vitro Fert Embryo Transf 8:179–190CrossRefPubMed

15.

Cohen J, Alikani M, Trowbridge J, Rosenwaks Z (1992) Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod 7:685–691CrossRefPubMed

16.

Hammadeh ME, Fischer-Hammadeh C, Ali KR (2011) Assisted hatching in assisted reproduction: a state of the art. J Assist Reprod Genet 28:119–128CrossRefPubMed

17.

O’Neill C (1997) Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro. Biol Reprod 56:229–237CrossRefPubMed

18.

Gu YF, Lu CF, Lin G, Lu GX (2010) A comparative analysis of the zona pellucida birefringence of fresh and frozen-thawed human embryos. Reproduction 139:121–127CrossRefPubMed

19.

Germond M, Nocera D, Senn A, Rink K, Delacréaz G, Pedrazzini T et al (1996) Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48 μm diode laser beam. Hum Reprod 11:1043CrossRefPubMed

20.

Petersen CG, Mauri AL, Baruffi RL, Oliveira JB, Felipe V, Massaro FC et al (2006) Laser-assisted hatching of cryopreserved-thawed embryos by thinning one quarter of the zona. Reprod BioMed Online 13:668–675CrossRefPubMed

21.

Zhang XJ, Yang YZ, Lv Q, Min LH, Li XL, Bai P (2009) Effect of the size of zona pellucida thinning by laser assisted hatching on clinical outcome of human frozen-thawed embryo transfers. Cryo-Letters 30:455–461PubMed

22.

Ng EH, Naveed F, Lau EY, Yeung WS, Chan CC, Tang OS, Ho PC (2005) A randomized double-blind controlled study of the effectiveness of laser-assisted hatching on implantation and pregnancy rates of frozen-warmed embryo transfer at the cleavage stage. Hum Reprod 20:979–985CrossRefPubMed

23.

Frydman N, Madoux SL, Duvernoy C, Feyereisen E, Le DA, Tachdjian G et al (2006) A randomized double-blind controlled study on the efficacy of laser zona pellucida thinning on live birth rates in cases of advanced female age. Hum Reprod 21:2131–2135CrossRefPubMed

24.

Sagoskin A, Levy MM, Richter K, Widra E (2007) Laser assisted hatching in good prognosis patients undergoing in vitro fertilization-embryo transfer: a randomized controlled trial. Fertil Steril 87:283–287CrossRefPubMed

25.

Razi MH, Halvaei I, Razi Y (2013) Laser assisted zona hatching does not improve live birth rate in patients undergoing their first ICSI cycles. Iran J Reprod Med 11:1021PubMedPubMedCentral

26.

Ge HS, Zhou W, Zhang W, Lin JJ (2008) Impact of assisted hatching on fresh and frozen-warmed embryo transfer cycles: a prospective, randomized study. Reprod BioMed Online 16:589–596CrossRefPubMed

27.

Debrock S, Peeraer K, Spiessens C, Willemen D, De Loecker P, D' Hooghe TM (2011) The effect of modified quarter laser-assisted zona thinning on the implantation rate per embryo in frozen/vitrified-thawed/warmed embryo transfer cycles: a prospective randomized controlled trial. Hum Reprod 26:1997–2007CrossRefPubMed

28.

Tao J, Tamis R, Fink K, Williams B, Nelson-White T, Craig R (2002) The neglected morula/compact stage embryo transfer. Hum Reprod 17:1513–1518CrossRefPubMed

29.

Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T et al (1998) Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53–58CrossRefPubMed

30.

Mantoudis E, Podsiadly BT, Gorgy A, Venkat G, Craft IL (2001) A comparison between quarter, partial and total laser zona thinning in selected infertility patients. Hum Reprod 16:2182–2186CrossRefPubMed

31.

Hiraoka K, Hiraoka K, Horiuchi T, Kusuda T, Okano S, Kinutani M, Kinutani K (2009) Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective randomized study. J Assist Reprod Genet 26:515–521CrossRefPubMedPubMedCentral

32.

Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB (2000) Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril 73(6):1155–1158CrossRefPubMed

33.

Yan Z, Liang H, Deng L et al (2015) Eight-shaped hatching increases the risk of inner cell mass splitting in extended mouse embryo culture. PLoS One 10(12):e0145172CrossRefPubMedPubMedCentral

34.

Tian Y, Shen L, Gao Y, Yamauchi T, Shen XM, Ma N (2007) Comparison of 4′, 6′-diamidino-2-phenylindole and Giemsa stainings in preimplantation mouse embryos micronucleus assay including a triple dose study. Ind Health 45:343CrossRefPubMed

35.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(−Delta Delta C) method. Methods 25:402–408CrossRef

36.

Rulicke T, Haenggli A, Rappold K, Moehrlen U, Stallmach T (2006) No transuterine migration of fertilised ova after unilateral embryo transfer in mice. Reprod Fertil Dev 18:885–891CrossRefPubMed

37.

Tucker MJ, Luecke NM, Wiker SR, Wright G (1993) Chemical removal of the outside of the ZP of day 3 human embryos has no impact on implantation rate. J Assist Reprod Genet 10:187–191CrossRefPubMed

38.

Pelletier C, Keefe DL, Trimarchi JR (2004) Noninvasive polarized light microscopy quantitatively distinguishes the multilaminar structure of the ZP of living human eggs and embryos. Fertil Steril 81(Suppl 1):850–856CrossRefPubMed

39.

Balaban B, Urman B, Yakin K, Isiklar A (2006) Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study. Hum Reprod 21:2136–2140CrossRefPubMed

40.

Ng EH, Lau EY, Yeung WS, Cheung TM, Tang OS, Ho PC (2008) Randomized double-blind comparison of laser zona pellucida thinning and breaching in frozen-thawed embryo transfer at the cleavage stage. Fertil Steril 89:1147–1153CrossRefPubMed

41.

Park SB, Kim HJ, Choi YB, Ahn KH, Lee KH, Yang JB, Yu CS, Seo BB (2014) The effect of various assisted hatching techniques on the mouse early embryo development. Clin Exp Reprod Med 41:68–74CrossRefPubMedPubMedCentral

42.

Sathananthan H, Menezes J, Gunasheela S (2003) Mechanics of human blastocyst hatching in vitro. Reprod BioMed Online 7:228–234CrossRefPubMed

43.

Khalifa EA, Tucker MJ, Hunt P (1992) Cruciate thinning of the zona pellucida for more successful enhancement of blastocyst hatching in the mouse. Hum Reprod 7:532–536CrossRefPubMed

44.

Kim HJ, Lee KH, Park SB, Choi YB, Yang JB (2015) The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification. Clin Exp Reprod Med 42:94–100CrossRefPubMedPubMedCentral

45.

Kanyo K, Konc J (2003) A follow-up study of children born after diode laser assisted hatching. Eur J Obstet Gynecol Reprod Biol 110:176–180CrossRefPubMed

46.

Bhuiyan MM, Kang SK, Lee BC (2007) Effects of fructose supplementation in chemically defined protein-free medium on development of bovine in vitro fertilized embryos. Anim Reprod Sci 102:137–144CrossRefPubMed

47.

Schoolcraft WB, Schlenker T, Gee M, Jones GS, Jones HJ (1994) Assisted hatching in the treatment of poor prognosis in vitro fertilization candidates. Fertil Steril 62:551–554CrossRefPubMed

Title: The impacts of laser zona thinning on hatching and implantation of vitrified-warmed mouse embryos
Authors: Zhengyuan Huang
Jinghao Liu
Lei Gao
Qingrui Zhuan
Yuxi Luo
Shien Zhu
Kaiyu Lei
Xiangwei Fu
Publication date: 01-07-2019
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 5/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2681-8

At a glance: The STEP trials

Springer Medicine

The impacts of laser zona thinning on hatching and implantation of vitrified-warmed mouse embryos

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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