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01-04-2018 | Original Article

Comparison of the effect of root surface modification with citric acid, EDTA, and aPDT on adhesion and proliferation of human gingival fibroblasts and osteoblasts: an in vitro study

Authors: Rafael Ferreira, Renato Taddei de Toledo Barros, Paula Stephania Brandão Hage Karam, Adriana Campos Passanezi Sant’Ana, Sebastião Luiz Aguiar Greghi, Maria Lucia Rubo de Rezende, Mariana Schutzer Ragghianti Zangrando, Rodrigo Cardoso de Oliveira, Carla Andreotti Damante

Published in: Lasers in Medical Science | Issue 3/2018

Abstract

Root demineralization is used in Periodontics as an adjuvant for mechanical treatment. The aim of this study was to evaluate the effects of root surface modification with mechanic, chemical, and photodynamic treatments on adhesion and proliferation of human gingival fibroblasts and osteoblasts. Root fragments were treated by scaling and root planing (C—control group), EDTA (pH 7), citric acid plus tetracycline (CA—pH 1), and antimicrobial photodynamic therapy (aPDT) with toluidine blue O and red laser (pH 4). Cells were seeded (104 cells/well, 6th passage) on root fragments of each experimental group and cultured for 24, 48, and 72 h. Cells were counted in scanning electron microscopy images by a calibrated examiner. For fibroblasts, the highest number of cells were present at 72-h period (p < 0.05). EDTA group showed a very low number of cells in relation to CA group (p < 0.05). CA and aPDT group presented higher number of cells in all periods, but without differences between other treatment groups (p > 0.05). For osteoblasts, there was a significant increase in cell numbers for aPDT group at 72 h (p < 0.05). In conclusion, aPDT treatment provided a positive stimulus to osteoblast growth, while for fibroblasts, aPDT and CA had a tendency for higher cell growth.

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Title: Comparison of the effect of root surface modification with citric acid, EDTA, and aPDT on adhesion and proliferation of human gingival fibroblasts and osteoblasts: an in vitro study
Authors: Rafael Ferreira
Renato Taddei de Toledo Barros
Paula Stephania Brandão Hage Karam
Adriana Campos Passanezi Sant’Ana
Sebastião Luiz Aguiar Greghi
Maria Lucia Rubo de Rezende
Mariana Schutzer Ragghianti Zangrando
Rodrigo Cardoso de Oliveira
Carla Andreotti Damante
Publication date: 01-04-2018
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2018
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2395-3

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Comparison of the effect of root surface modification with citric acid, EDTA, and aPDT on adhesion and proliferation of human gingival fibroblasts and osteoblasts: an in vitro study

Abstract

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Abstract

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