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01-12-2017 | Original Article

Leishmanicidal effect of antiparasitic photodynamic therapy—ApPDT on infected macrophages

Authors: Susana de Oliveira, Evaristo João da Ordem Trahamane, Juliana Monteiro, Gustavo Pires Santos, Pedro Crugeira, Fernando Sampaio, Camila Oliveira, Manoel Barral Neto, Antônio Pinheiro

Published in: Lasers in Medical Science | Issue 9/2017

Abstract

The aim of this study is to evaluate the effects of ApPDT (antiparasitic photodynamic therapy) on the interaction of Leishmania braziliensis with J774 macrophages, used as a photosensitizer, methylene blue associated with red laser. The tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and ApPDT. The photosensitizer used was the methylene blue at concentration of 12.5 μg/mL. The parameters of the laser were λ = 660 nm, 40 mW, and 8.4 J/cm². Samples are analyzed by optical microscopy through the identification and counting of infected and uninfected macrophages, parasite load, infectivity, and infection index. Statistical analysis used ANOVA test with Tukey post-test, being considered statistically significant p < 0.05. The analysis of the interaction tests shows that the infection rate in the ApPDT group in relation to the control group presents a statistically significant reduction (p < 0.0001) of 71% at both 24 and 48 h (p < 0.0001) of 62%. ApPDT reduces the number of macrophages infected by Leishmania braziliensis, as well as the number of intracellular parasites, being a possible alternative therapy in the treatment of cutaneous leishmaniosis.

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Title: Leishmanicidal effect of antiparasitic photodynamic therapy—ApPDT on infected macrophages
Authors: Susana de Oliveira
Evaristo João da Ordem Trahamane
Juliana Monteiro
Gustavo Pires Santos
Pedro Crugeira
Fernando Sampaio
Camila Oliveira
Manoel Barral Neto
Antônio Pinheiro
Publication date: 01-12-2017
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 9/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2292-9

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Leishmanicidal effect of antiparasitic photodynamic therapy—ApPDT on infected macrophages

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