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Lasers in Medical Science
Published in: Lasers in Medical Science 7/2017

01-09-2017 | Original Article

In vitro studies to evaluate the effect of varying culture conditions and IPL fluencies on tenocyte activities

Authors: Jihad A. M. Alzyoud, Ilyas M. Khan, Sarah G. Rees

Published in: Lasers in Medical Science | Issue 7/2017

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Abstract

Tendons are dense, fibrous connective tissues which carry out the essential physiological role of transmitting mechanical forces from skeletal muscle to bone. From a clinical perspective, tendinopathy is very common, both within the sporting arena and amongst the sedentary population. Studies have shown that light therapy may stimulate tendon healing, and more recently, intense pulsed light (IPL) has attracted attention as a potential treatment modality for tendinopathy; however, its mechanism of action and effect on the tendon cells (tenocytes) is poorly understood. The present study therefore investigates the influence of IPL on an in vitro bovine tendon model. Tenocytes were irradiated with IPL at different devise settings and under variable culture conditions (e.g. utilising cell culture media with or without the pH indicator dye phenol red), and changes in tenocyte viability and migration were subsequently investigated using Alamar blue and scratch assays, respectively. Our data demonstrated that IPL fluencies of up to 15.9 J/cm2 proved harmless to the tenocyte cultures (this was the case using culture media with or without phenol red) and resulted in a significant increase in cell viability under certain culture conditions. Furthermore, IPL treatment of tenocytes did not affect the rate of cell migration. This study demonstrates that irradiation with IPL is not detrimental to the tenocytes and may increase their viability under certain conditions, thus validating our in vitro model. Further studies are required to elucidate the effects of IPL application in the clinical situation.
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Metadata
Title
In vitro studies to evaluate the effect of varying culture conditions and IPL fluencies on tenocyte activities
Authors
Jihad A. M. Alzyoud
Ilyas M. Khan
Sarah G. Rees
Publication date
01-09-2017
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 7/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-017-2279-6

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