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Lasers in Medical Science
Published in: Lasers in Medical Science 4/2014

01-07-2014 | Original Article

Low-level laser irradiation stimulates tenocyte proliferation in association with increased NO synthesis and upregulation of PCNA and cyclins

Authors: Wen-Chung Tsai, Ju-Wen Cheng, Jean-Lon Chen, Chen-Yin Chen, Hsiang-Ning Chang, Yu-Hsin Liao, Miao-Sui Lin, Jong-Hwei S. Pang

Published in: Lasers in Medical Science | Issue 4/2014

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Abstract

Low-level laser therapy is commonly used to treat tendinopathy or tendon injury. Tendon healing requires tenocyte migration to the repair site, followed by proliferation and synthesis of the extracellular matrix. There are few evidence to elucidate that low-level laser promote tenocyte proliferation. This study was designed to determine the effect of laser on tenocyte proliferation. Furthermore, the association of this effect with secretion of nitric oxide (NO) and the expressions of proliferating cell nuclear antigen (PCNA) and cyclins D1, E, A, and B1 was investigated. Tenocytes intrinsic to rat Achilles tendon were treated with low-level laser (660 nm). Tenocyte proliferation was evaluated by MTT assay and immunocytochemistry with Ki-67 stain. NO in the conditioned medium was measured by ELISA. Western blot analysis was used to evaluate the protein expressions of PCNA and cyclins D1, E, A, and B1. The results revealed that tenocytes proliferation was enhanced dose dependently by laser. NO secretion was increased after laser treatment. PCNA and cyclins E, A, and B1 were upregulated by laser. In conclusion, low-level laser irradiation stimulates tenocyte proliferation in a process that is mediated by upregulation of NO, PCNA, and cyclins E, A, and B1.
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Metadata
Title
Low-level laser irradiation stimulates tenocyte proliferation in association with increased NO synthesis and upregulation of PCNA and cyclins
Authors
Wen-Chung Tsai
Ju-Wen Cheng
Jean-Lon Chen
Chen-Yin Chen
Hsiang-Ning Chang
Yu-Hsin Liao
Miao-Sui Lin
Jong-Hwei S. Pang
Publication date
01-07-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 4/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1528-1

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