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01-04-2017 | Original Article

Repeated applications of photodynamic therapy on Candida glabrata biofilms formed in acrylic resin polymerized

Authors: Lírian Silva de Figueiredo Freitas, Rodnei Dennis Rossoni, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira

Published in: Lasers in Medical Science | Issue 3/2017

Abstract

Previous studies have been suggested that photodynamic therapy (PDT) can be used as an adjuvant treatment for denture stomatitis. In this study, we evaluated the effects of multiple sessions of PDT on Candida glabrata biofilms in specimens of polymerized acrylic resin formed after 5 days. Subsequently, four applications of PDT were performed on biofilms in 24-h intervals (days 6–9). Also, we evaluated two types of PDT, including application of laser and methylene blue or light-emitting diode (LED) and erythrosine. The control groups were treated with physiological solution. The effects of PDT on biofilm were evaluated after the first and fourth application of PDT. The biofilm analysis was performed by counting the colony-forming units. The results showed that between the days 6 and 9, the biofilms not treated by PDT had an increase of 5.53 to 6.05 log (p = 0.0271). Regarding the treatments, after one application of PDT, the biofilms decreased from 5.53 to 0.89 log. When it was done four applications, the microbial reduction ranged from 6.05 log to 0.11 log. We observed that one application of PDT with laser or LED caused a reduction of 3.36 and 4.64 compared to the control groups, respectively (p = 0.1708). When it was done four applications of PDT, the reductions achieved were 1.57 for laser and 5.94 for LED (p = 0.0001). It was concluded that repeated applications of PDT on C. glabrata biofilms showed higher antimicrobial activity compared to single application. PDT mediated by LED and erythrosine was more efficient than the PDT mediated by laser and methylene blue.

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Title: Repeated applications of photodynamic therapy on Candida glabrata biofilms formed in acrylic resin polymerized
Authors: Lírian Silva de Figueiredo Freitas
Rodnei Dennis Rossoni
Antonio Olavo Cardoso Jorge
Juliana Campos Junqueira
Publication date: 01-04-2017
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2147-4

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Repeated applications of photodynamic therapy on Candida glabrata biofilms formed in acrylic resin polymerized

Abstract

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