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01-02-2016 | Original Article

Safety assessment of trans-tympanic photobiomodulation

Authors: Tae-Hyun Moon, Min Young Lee, Jae Yun Jung, Jin-Chul Ahn, So-Young Chang, Phil-Sang Chung, Chung-Ku Rhee, Yoon-Hwan Kim, Myung-Whan Suh

Published in: Lasers in Medical Science | Issue 2/2016

Abstract

We evaluated functional and morphological changes after trans-tympanic laser application using several different powers of photobiomodulation (PBM). The left (L) ears of 17 rats were irradiated for 30 min daily over 14 days using a power density of 909.1 (group A, 5040 J), 1136.4 (group B, 6300 J), and 1363.6 (group C, 7560 J) mW/cm². The right (N) ears served as controls. The safety of PBM was determined by endoscopic findings, auditory brainstem response (ABR) thresholds, and histological images of hair cells using confocal microscopy, and light microscopic images of the external auditory canal (EAC) and tympanic membrane (TM). Endoscopic findings revealed severe inflammation in the TM of C group; no other group showed damage in the TM. No significant difference in ABR threshold was found in the PBM-treated groups (excluding the group with TM damage). Confocal microscopy showed no histological difference between the AL and AN, or BL and BN groups. However, light microscopy showed more prominent edema, inflammation, and vascular congestion in the TM of BL ears. This study found a dose-response relationship between laser power parameters and TM changes. These results will be useful for defining future allowance criteria for trans-tympanic laser therapies.

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Title: Safety assessment of trans-tympanic photobiomodulation
Authors: Tae-Hyun Moon
Min Young Lee
Jae Yun Jung
Jin-Chul Ahn
So-Young Chang
Phil-Sang Chung
Chung-Ku Rhee
Yoon-Hwan Kim
Myung-Whan Suh
Publication date: 01-02-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1851-1

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Safety assessment of trans-tympanic photobiomodulation

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