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01-01-2016 | Original Article

A grid matrix-based Raman spectroscopic method to characterize different cell milieu in biopsied axillary sentinel lymph nodes of breast cancer patients

Authors: Dipasree Som, Megha Tak, Mohit Setia, Asawari Patil, Amit Sengupta, C. Murali Krishna Chilakapati, Anurag Srivastava, Vani Parmar, Nita Nair, Rajiv Sarin, R. Badwe

Published in: Lasers in Medical Science | Issue 1/2016

Abstract

Raman spectroscopy which is based upon inelastic scattering of photons has a potential to emerge as a noninvasive bedside in vivo or ex vivo molecular diagnostic tool. There is a need to improve the sensitivity and predictability of Raman spectroscopy. We developed a grid matrix-based tissue mapping protocol to acquire cellular-specific spectra that also involved digital microscopy for localizing malignant and lymphocytic cells in sentinel lymph node biopsy sample. Biosignals acquired from specific cellular milieu were subjected to an advanced supervised analytical method, i.e., cross-correlation and peak-to-peak ratio in addition to PCA and PC-LDA. We observed decreased spectral intensity as well as shift in the spectral peaks of amides and lipid bands in the completely metastatic (cancer cells) lymph nodes with high cellular density. Spectral library of normal lymphocytes and metastatic cancer cells created using the cellular specific mapping technique can be utilized to create an automated smart diagnostic tool for bench side screening of sampled lymph nodes. Spectral library of normal lymphocytes and metastatic cancer cells created using the cellular specific mapping technique can be utilized to develop an automated smart diagnostic tool for bench side screening of sampled lymph nodes supported by ongoing global research in developing better technology and signal and big data processing algorithms.

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Title: A grid matrix-based Raman spectroscopic method to characterize different cell milieu in biopsied axillary sentinel lymph nodes of breast cancer patients
Authors: Dipasree Som
Megha Tak
Mohit Setia
Asawari Patil
Amit Sengupta
C. Murali Krishna Chilakapati
Anurag Srivastava
Vani Parmar
Nita Nair
Rajiv Sarin
R. Badwe
Publication date: 01-01-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 1/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1830-6

ACC 2024 Congress

Springer Medicine

A grid matrix-based Raman spectroscopic method to characterize different cell milieu in biopsied axillary sentinel lymph nodes of breast cancer patients

Abstract

ACC 2024 Congress

Springer Medicine

Abstract

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