Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 5/2015

01-07-2015 | Original Article

Layer-resolved colorectal tissues using nonlinear microscopy

Authors: Lianhuang Li, Hongsheng Li, Zhifen Chen, Shuangmu Zhuo, Changyin Feng, Yinghong Yang, Guoxian Guan, Jianxin Chen

Published in: Lasers in Medical Science | Issue 5/2015

Login to get access

Abstract

In this work, multiphoton microscopy (MPM), based on the nonlinear optical processes two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), was extended to evaluate the feasibility of using MPM to distinguish layers of the bowel wall. It was found that MPM has the ability to identify the four-layer microstructures of colorectal tissues including mucosa, submucosa, muscularis propria, and serosa as there are many intrinsic signal sources in each layer. Our results also showed the capability of using the quantitative analyses of MPM images for quantifying some feature parameters including the nuclear area, nuclear-to-cytoplasmic ratio, and optical redox ratio. This work demonstrates that MPM has the potential in noninvasively monitoring the development and progression of colorectal diseases and then guiding effective treatment.
Literature
1.
American Cancer Society (2014) Cancer facts & figures 2014. American Cancer Society, Atlanta
2.
3.
McKeown E, Nelson DW, Johnson EK et al (2014) Current approaches and challenges for monitoring treatment response in colon and rectal cancer. J Cancer 5:31–43PubMedCentralPubMedCrossRef
4.
5.
Tanaka K, Toiyama Y, Okugawa Y et al (2014) In vivo optical imaging of cancer metastasis using multiphoton microscopy: a short review. Am J Transl Res 6:179–187PubMedCentralPubMed
6.
Campagnola PJ, Loew LM (2003) Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms. Nat Biotechnol 21:1356–1360PubMedCrossRef
7.
8.
So PTC, Dong CY, Masters BR et al (2000) Two-photon excitation fluorescence microscopy. Annu Rev Biomed Eng 2:399–429PubMedCrossRef
9.
Schurmann S, Foersch S, Atreya R et al (2013) Label-free imaging of inflammatory bowel disease using multiphoton microscopy. Gastroenterology 145:514–516PubMedCrossRef
10.
Birk JW, Tadros M, Moezardalan K et al (2014) Second harmonic generation imaging distinguishes both high-grade dysplasia and cancer from normal colonic mucosa. Dig Dis Sci 59:1529–1534PubMedCrossRef
11.
Cicchi R, Sturiale A, Nesi G et al (2013) Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma. Biomed Opt Express 4:1204–1213PubMedCentralPubMedCrossRef
12.
Liu NR, Chen JX, Chen G et al (2012) Detecting the imaging characteristics of colorectal carcinoma invading the muscularis propria with multiphoton microscopy. Laser Phys Lett 9:155–159CrossRef
13.
Zhuo SM, Chen JX, Luo TS et al (2006) Multimode nonlinear optical imaging of the dermis in ex vivo human skin based on the combination of multichannel mode and Lambda mode. Opt Express 14:7810–7820PubMedCrossRef
14.
Chen JX, Zhuo SM, Chen R et al (2007) Depth-resolved spectral imaging of rabbit oesophageal tissue based on two-photon excited fluorescence and second-harmonic generation. New J Phys 9:212–212CrossRef
15.
Zhuo SM, Zheng LQ, Chen JX et al (2010) Depth-cumulated epithelial redox ratio and stromal collagen quantity as quantitative intrinsic indicators for differentiating normal, inflammatory, and dysplastic epithelial tissues. Appl Phys Lett 97:173701CrossRef
16.
Monici M (2005) Cell and tissue autofluorescence research and diagnostic applications. Biotechnol Annu Rev 11:227–256PubMed
17.
18.
Kennedy JC, Nadeau P, Petryka ZJ et al (1984) Clearance times of porphyrin derivatives from mice as measured by in vivo fluorescence spectroscopy. Photochem Photobiol 39:729–734CrossRef
19.
Wang HW, Willis J, Canto MIF et al (1999) Quantitative laser scanning confocal autofluorescence microscopy of normal, premalignant, and malignant colonic tissues. Ieee Trans Biomed Eng 46:1246–1252PubMedCrossRef
20.
21.
Matteini P, Ratto F, Rossi F et al (2009) Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging. Opt Express 17:4868–4878PubMedCrossRef
22.
Eidelman S, Lagunoff D (1972) The morphology of the normal human rectal biopsy. Hum Pathol 3:389–401PubMedCrossRef
23.
Zheng W, Li D, Li S et al (2011) Diagnostic value of nonlinear optical signals from collagen matrix in the detection of epithelial precancer. Opt Lett 36:3620–3622PubMedCrossRef
24.
Boulesteix T, Pena AM, Pages N et al (2006) Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure. Cytometry Part A 69A:20–26CrossRef
25.
Arkill KP, Moger J, Winlove CP (2010) The structure and mechanical properties of collecting lymphatic vessels: an investigation using multimodal nonlinear microscopy. J Anat 216:547–555PubMedCentralPubMedCrossRef
26.
Zhuo SM, Chen JX, Luo TS et al (2009) Two-layered multiphoton microscopic imaging of cervical tissue. Lasers Med Sci 24:359–363PubMedCrossRef
27.
Rudin M, Weissleder R (2003) Molecular imaging in drug discovery and development. Nat Rev Drug Discov 2:123–131PubMedCrossRef
28.
Skala MC, Squirrell JM, Vrotsos KM et al (2005) Multiphoton microscopy of endogenous fluorescence differentiates normal, precancerous, and cancerous squamous epithelial tissues. Cancer Res 65:1180–1186PubMedCentralPubMedCrossRef
29.
Rogart JN, Nagata J, Loeser CS et al (2008) Multiphoton imaging can be used for microscopic examination of intact human gastrointestinal mucosa ex vivo. Clin Gastroenterol Hepatol 6:95–101PubMedCentralPubMedCrossRef
30.
Hovhannisyan VA, Su PJ, Lin SJ et al (2009) Quantifying thermodynamics of collagen thermal denaturation by second harmonic generation imaging. Appl Phys Lett 94:233902CrossRef
31.
Cicchi R, Crisci A, Cosci A et al (2010) Time- and spectral-resolved two-photon imaging of healthy bladder mucosa and carcinoma in situ. Opt Express 18:3840–3849PubMedCrossRef
Metadata
Title
Layer-resolved colorectal tissues using nonlinear microscopy
Authors
Lianhuang Li
Hongsheng Li
Zhifen Chen
Shuangmu Zhuo
Changyin Feng
Yinghong Yang
Guoxian Guan
Jianxin Chen
Publication date
01-07-2015
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 5/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-015-1767-9

Other articles of this Issue 5/2015

Lasers in Medical Science 5/2015 Go to the issue

Original Article

Er:YAG laser irradiation to control the progression of enamel erosion: an in situ study

Brief Report

1064 Nd:YAG laser for the treatment of chronic paronychia: a pilot study

Original Article

Method to control depth error when ablating human dentin with numerically controlled picosecond laser: a preliminary study

Original Article

A comparative study of toluidine blue-mediated photodynamic therapy versus topical corticosteroids in the treatment of erosive-atrophic oral lichen planus: a randomized clinical controlled trial

Original Article

Performance analysis of the beam shaping method on optical auditory neural stimulation in vivo

Original Article

Diagnostic performance of a new red light LED device for approximal caries detection