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01-05-2009 | Origianal Article

Two-layered multiphoton microscopic imaging of cervical tissue

Authors: Shuangmu Zhuo, Jianxin Chen, Tianshu Luo, Xingshan Jiang, Shusen Xie, Rong Chen

Published in: Lasers in Medical Science | Issue 3/2009

Abstract

We demonstrate the application of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals in imaging two-layered structures of cervical tissue in mice. It was found that MPM is effective for visualizing two-layered cervical microstructures, monitoring the metabolic activity and the density (nuclear-to-cytoplasmic) of epithelial cells, measuring the size of epithelial cell nuclei and the thickness of the epithelial layer, and quantifying collagen in the stroma. These results suggest that, with the advent of clinical portability of typical multiphoton endoscopes, the MPM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of cervical cancer.

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Title: Two-layered multiphoton microscopic imaging of cervical tissue
Authors: Shuangmu Zhuo
Jianxin Chen
Tianshu Luo
Xingshan Jiang
Shusen Xie
Rong Chen
Publication date: 01-05-2009
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2009
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0570-2

At a glance: The STEP trials

Springer Medicine

Two-layered multiphoton microscopic imaging of cervical tissue

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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