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01-11-2015 | Original Article

Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat’s TMJ induced by carrageenan

Authors: Isabele C. V. de Castro, Cristiane B. Rosa, Carolina M. Carvalho, Juliana S. Aragão, Maria Cristina T. Cangussu, Jean N. dos Santos, Antonio L. B. Pinheiro

Published in: Lasers in Medical Science | Issue 8/2015

Abstract

Temporomandibular disorders (TMDs) are mostly inflammatory conditions widespread in the population. Previous studies have shown positive effects of either laser or light-emitting diode (LED) phototherapies on treating TMDs, but their action and mechanism in the inflammatory infiltrate of the temporomandibular joint are still poorly understood. The aim of this study was to assess, through histological analysis, the effectiveness of using laser light (λ 780 nm, 70 mW, continous wave (CW), 10 J) and LED (λ 850 ± 10 nm, 100 mW, CW, 10 J) on the inflammation of the temporomandibular joint of rats induced by carrageenan. Forty-five animals were divided into three groups with five animals each according to the experimental times of 2, 3, and 7 days: inflammation, inflammation+laser phototherapy, and inflammation+LED phototherapy. The first irradiation was performed 24 h after induction with an interval of 48 h between sessions. After animal death, specimens were processed and stained with hematoxylin-eosin (HE) and picrosirius. Then, the samples were examined histologically. Data were statistically analyzed. The inflammation group showed mild to moderate chronic inflammatory infiltrate between bone trabecules of the condyle. Over the time course of the study in the laser group, the region of the condyle presented mild chronic inflammation and intense vascularization. In the LED group, the condyle showed aspects of normality and absence of inflammation in some specimens. In all the time points, the laser-irradiated groups showed greater amount of collagen deposition in the condyle (p = 0.04) and in the disc (p = 0.03) when compared to the inflammation and LED groups, respectively. Laser- and LED-treated groups demonstrate a smaller number of layers of the synovial membrane when compared to the non-irradiated groups. It was concluded that, in general, laser and LED phototherapies resulted in a reduction of inflammatory infiltrate in the temporomandibular joint of rat.

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Title: Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat’s TMJ induced by carrageenan
Authors: Isabele C. V. de Castro
Cristiane B. Rosa
Carolina M. Carvalho
Juliana S. Aragão
Maria Cristina T. Cangussu
Jean N. dos Santos
Antonio L. B. Pinheiro
Publication date: 01-11-2015
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 8/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1748-z

At a glance: The STEP trials

Springer Medicine

Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat’s TMJ induced by carrageenan

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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