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01-05-2014 | Original Article

Low-level laser therapy and light-emitting diode effects in the secretion of neuropeptides SP and CGRP in rat skin

Authors: Bernardo Hochman, Carlos E. Pinfildi, Michele A. Nishioka, Fabianne Furtado, Silvilena Bonatti, Paola K. P. Monteiro, Arainy S. Antunes, Paulo R. Quieregatto, Richard E. Liebano, Gerson Chadi, Lydia Masako Ferreira

Published in: Lasers in Medical Science | Issue 3/2014

Abstract

The phototherapy effects in the skin are related to biomodulation, usually to accelerate wound healing. However, there is no direct proof of the interrelation between the effects of low-level laser therapy (LLLT) and light-emitting diode (LED) in neuropeptide secretion, these substances being prematurely involved in the neurogenic inflammation phase of wound healing. This study therefore focused on investigating LLLT and LED in Calcitonin gene-related peptide (CGRP) and substance P (SP) secretion in healthy rat skin. Forty rats were randomly distributed into five groups with eight rats each: Control Group, Blue LED Group (470 nm, 350 mW power), Red LED Group (660 nm, 350 mW power), Red Laser Group (660 nm, 100 mW power), and Infrared Laser Group (808 nm, 100 mW power) (DMC® Equipamentos Ltda., São Carlos, São Paulo, Brazil). The skin of the animals in the experimental groups was irradiated using the punctual contact technique, with a total energy of 40 J, single dose, standardized at one point in the dorsal region. After 14 min of irradiation, the skin samples were collected for CGRP and SP quantification using western blot analysis. SP was released in Infrared Laser Group (p = 0.01); there was no difference in the CGRP secretion among groups. Infrared (808 nm) LLLT enhances neuropeptide SP secretion in healthy rat skin.

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Title: Low-level laser therapy and light-emitting diode effects in the secretion of neuropeptides SP and CGRP in rat skin
Authors: Bernardo Hochman
Carlos E. Pinfildi
Michele A. Nishioka
Fabianne Furtado
Silvilena Bonatti
Paola K. P. Monteiro
Arainy S. Antunes
Paulo R. Quieregatto
Richard E. Liebano
Gerson Chadi
Lydia Masako Ferreira
Publication date: 01-05-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-013-1494-z

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Low-level laser therapy and light-emitting diode effects in the secretion of neuropeptides SP and CGRP in rat skin

Abstract

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Abstract

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