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Lasers in Medical Science
Published in: Lasers in Medical Science 1/2013

01-01-2013 | Original Article

The effect of He-Ne and Ga-Al-As laser light on the healing of hard palate mucosa of mice

Authors: Farahnaz Fahimipour, Mina Mahdian, Behzad Houshmand, Mohammad Asnaashari, Alireza Naser Sadrabadi, Seid Emadadin Najafi Farashah, Seid Mohsen Mousavifard, Arash Khojasteh

Published in: Lasers in Medical Science | Issue 1/2013

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Abstract

Low-level laser therapy (LLLT) has been used to accelerate wound healing, yet questions remain concerning its therapeutic applications. This study aimed to compare the healing efficacy of helium-neon (He-Ne) red light (laser) and gallium aluminum arsenide (Ga-Al-As) infrared lasers at two different doses on hard palate wounds. In a randomized controlled study, 75 adult male mice were divided into five groups of 15 each, after undergoing identical surgical procedures; a control group, with no laser irradiation; HD1 and HD2 groups, treated with He-Ne laser (wavelengths 632.8 nm, power 5 mW, and spot size 0.02 cm2) at doses of 4 J/cm2 and 7.5 J/cm2 respectively; and GD1 and GD2 groups, treated with Ga-Al-As laser (wavelengths 830 nm, peak power 25 mW, and spot size 0.10 cm2) at the doses of 4 J/cm2 and 7.5 J/cm2, respectively. Five animals from each group were killed on the third, seventh, and 14 days after surgery, and biopsies were made for histological analysis. On the 3rd and 7th day after the surgery, the number of polymorphonuclear cells (PMN) in HD1, HD2, GD1, and GD2 groups was significantly lower than that of the control group. On the 7th and 14th day, the fibroblasts and new blood vessels counts and collagen density fibers in HD1, HD2, GD1, and GD2 groups were also significantly higher than that of the control groups, and the fibroblast counts and collagen density fibers in HD1 and HD2 groups were higher than that of the GD1 and GD2 groups. LLLT with He-Ne laser compared to Ga-Al-As laser has a positive healing effect on hard palate gingival wounds in mice regardless of the radiation dose.
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Metadata
Title
The effect of He-Ne and Ga-Al-As laser light on the healing of hard palate mucosa of mice
Authors
Farahnaz Fahimipour
Mina Mahdian
Behzad Houshmand
Mohammad Asnaashari
Alireza Naser Sadrabadi
Seid Emadadin Najafi Farashah
Seid Mohsen Mousavifard
Arash Khojasteh
Publication date
01-01-2013
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 1/2013
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-012-1060-0

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