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European Journal of Clinical Microbiology & Infectious Diseases

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Open Access 01-12-2021 | Severe Acute Respiratory Syndrome Coronavirus | Brief Report

Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain

Authors: Nina Reiners, Carolin Schnurra, Henning Trawinski, Judith Kannenberg, Thomas Hermsdorf, Andrea Aebischer, Torsten Schöneberg, Sven Reiche, Christian Jassoy

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 12/2021

Abstract

SARS CoV-2 antibody assays measure antibodies against the viral nucleoprotein (NP) or spike protein. The study examined if testing of antibodies against both antigens increases the diagnostic sensitivity. Sera (N=98) from infected individuals were tested with ELISAs based on the NP, receptor-binding domain (RBD), or both proteins. The AUROCs were 0.958 (NP), 0.991 (RBD), and 0.992 (NP/RBD). The RBD- and NP/RBD-based ELISAs showed better performance than the NP-based assay. Simultaneous testing for antibodies against NP and RBD increased the number of true and false positives. If maximum diagnostic sensitivity is required, the NP/RBD-based ELISA is preferable. Otherwise, the RBD-based ELISA is sufficient.

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Title: Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain
Authors: Nina Reiners
Carolin Schnurra
Henning Trawinski
Judith Kannenberg
Thomas Hermsdorf
Andrea Aebischer
Torsten Schöneberg
Sven Reiche
Christian Jassoy
Publication date: 01-12-2021
Publisher: Springer Berlin Heidelberg
Keyword: Severe Acute Respiratory Syndrome Coronavirus
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 12/2021
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-021-04284-5

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