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European Journal of Clinical Microbiology & Infectious Diseases

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Open Access 01-11-2020 | Pneumonia | Brief Report

Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen

Authors: Ana Luísa Tomás, Fernando Cardoso, Bruno de Sousa, Olga Matos

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 11/2020

Abstract

Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results showed that IgM anti-Kex1 levels were found significantly increased in patients with Pneumocystis pneumonia (PcP) compared with non-PcP cases (p < 0.001), allowing a diagnostic performance of PcP with a 70.8% sensitivity and a 75.0% specificity. These results suggest that this Kex1-based ELISA is a promising tool toward the serodiagnosis of PcP when the standard methods are difficult to perform.

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Title: Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
Authors: Ana Luísa Tomás
Fernando Cardoso
Bruno de Sousa
Olga Matos
Publication date: 01-11-2020
Publisher: Springer Berlin Heidelberg
Keyword: Pneumonia
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 11/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03936-2

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