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European Journal of Clinical Microbiology & Infectious Diseases

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01-10-2020 | SARS-CoV-2 | Brief Report

The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Authors: Eric Farfour, Philippe Lesprit, Benoit Visseaux, Tiffany Pascreau, Emilie Jolly, Nadira Houhou, Laurence Mazaux, Marianne Asso-Bonnet, Marc Vasse, on behalf of the SARS-CoV-2 Foch Hospital study group

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 10/2020

Abstract

Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.

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Title: The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
Authors: Eric Farfour
Philippe Lesprit
Benoit Visseaux
Tiffany Pascreau
Emilie Jolly
Nadira Houhou
Laurence Mazaux
Marianne Asso-Bonnet
Marc Vasse
on behalf of the SARS-CoV-2 Foch Hospital study group
Publication date: 01-10-2020
Publisher: Springer Berlin Heidelberg
Keywords: SARS-CoV-2
COVID-19
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 10/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03930-8

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