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European Journal of Clinical Microbiology & Infectious Diseases

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Open Access 01-09-2020 | Antibiotic | Original Article

Emergence of linezolid-resistant Staphylococcus epidermidis in the tertiary children’s hospital in Cracow, Poland

Authors: Maja Kosecka-Strojek, Ewa Sadowy, Iwona Gawryszewska, Joanna Klepacka, Tomasz Tomasik, Michal Michalik, Waleria Hryniewicz, Jacek Miedzobrodzki

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 9/2020

Abstract

Coagulase-negative staphylococci, ubiquitous commensals of human skin, and mucous membranes represent important pathogens for immunocompromised patients and neonates. The increasing antibiotic resistance among Staphylococcus epidermidis is an emerging problem worldwide. In particular, the linezolid-resistant S. epidermidis (LRSE) strains are observed in Europe since 2014. The aim of our study was to genetically characterize 11 LRSE isolates, recovered mostly from blood in the University Children’s Hospital in Krakow, Poland, between 2015 and 2017. For identification of the isolates at the species level, we used 16S rRNA sequencing and RFLP of the saoC gene. Isolates were characterized phenotypically by determining their antimicrobial resistance patterns and using molecular methods such as PFGE, MLST, SCCmec typing, detection of the ica operon, and analysis of antimicrobial resistance determinants. All isolates were multidrug-resistant, including resistance to methicillin, and exhibited so-called PhLOPS_A phenotype. In PFGE, all isolates (excluding one from a catheter) represented identical patterns, were identified as ST2, and harbored the ica operon, responsible for biofilm formation. Linezolid resistance was associated with acquisition of A157R mutation in the ribosomal protein L3 and the presence of cfr gene. All isolates revealed new SCCmec cassette element composition. Recently, pediatric patients with serious staphylococcal infections are often treated with linezolid. The increasing linezolid resistance in bacterial strains becomes a real threat for patients, and monitoring such infections combined with surveillance and infection prevention programs is very important to decrease number of linezolid-resistant staphylococcal strains.

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Title: Emergence of linezolid-resistant Staphylococcus epidermidis in the tertiary children’s hospital in Cracow, Poland
Authors: Maja Kosecka-Strojek
Ewa Sadowy
Iwona Gawryszewska
Joanna Klepacka
Tomasz Tomasik
Michal Michalik
Waleria Hryniewicz
Jacek Miedzobrodzki
Publication date: 01-09-2020
Publisher: Springer Berlin Heidelberg
Keywords: Antibiotic
Linezolid
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 9/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03893-w

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