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European Journal of Clinical Microbiology & Infectious Diseases

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Open Access 01-08-2020 | Care | Original Article

Performance and ease of use of a molecular point-of-care test for influenza A/B and RSV in patients presenting to primary care

Authors: Jan Y Verbakel, Veerle Matheeussen, Katherine Loens, Mandy Kuijstermans, Herman Goossens, Margareta Ieven, Christopher C Butler

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 8/2020

Abstract

Annual influenza epidemics cause substantial morbidity and mortality, and the majority of patients with influenza-like illness present to primary care. Point-of-care influenza tests could support treatment decisions. It is critical to establish analytic performance of these platforms in real-life patient samples before uptake can be considered. We aimed to assess the analytical performance and ease of use of the cobas® Liat® PCR POCT in detecting influenza A/B and RSV in samples collected from patients with influenza-like illness in primary care. Sensitivity and specificity of the cobas® Liat® POCT are calculated in comparison with a commercial laboratory-based PCR test (Fast-Track Respiratory Pathogens 21 Plus kit (Fast-Track Diagnostics)). Samples with discordant results were analysed additionally by the RespiFinder 2Smart (PathoFinder) using an Extended Gold Standard (EGS). Acceptability was scored on a five-point Likert scale as well as a failure mode analysis of the cobas® Liat® POCT was performed. Nasal and oropharyngeal swabs were obtained from 140 children and nasopharyngeal swabs from 604 adults (744 patients). The cobas® Liat® POCT had a sensitivity and specificity of 100% (95% CI 99–100%) and 98.1% (95%CI 96.3–99%) for influenza A, 100% (95% CI 97.7–100%) and 99.7% (95%CI 98.7–99.9%) for influenza B and 100% (95% CI 87.1–100%) and 99.4% (95%CI 98.6–99.8%) for RSV, respectively. According to trained lab technicians, the cobas® Liat® POCT was considered easy-to-use, with a fast turn-around-time. Cobas® Liat® POCT is a promising decentralised test platform for influenza A/B and RSV in primary care as it provides fairly rapid results with excellent analytic performance. Point-of-care influenza tests could support treatment decisions in primary care. Cobas® Liat® POCT is a promising decentralised test platform for influenza A/B and RSV in primary care as it provides fairly rapid results with excellent analytic performance.

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Title: Performance and ease of use of a molecular point-of-care test for influenza A/B and RSV in patients presenting to primary care
Authors: Jan Y Verbakel
Veerle Matheeussen
Katherine Loens
Mandy Kuijstermans
Herman Goossens
Margareta Ieven
Christopher C Butler
Publication date: 01-08-2020
Publisher: Springer Berlin Heidelberg
Keywords: Care
Influenza
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 8/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03860-5

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