Skip to main content
Top
Published in: European Journal of Clinical Microbiology & Infectious Diseases 4/2013

01-04-2013 | Article

Gastric aspiration is not necessary for the diagnosis of pulmonary tuberculosis

Authors: P.-E. Bonnave, D. Raoult, M. Drancourt

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 4/2013

Login to get access

Abstract

Despite recommendations, gastric aspirate collected by invasive nasogastric aspiration is still routinely used for the direct detection of Mycobacterium tuberculosis in our institution. Reviewing 82 patients with culture-proven respiratory tuberculosis over a 28-month period, we observed no patient diagnosed solely by gastric aspirate analysis. Moreover, the diagnosis yield of gastric aspirate (60 %) did not significantly differ from that of stool specimen (64 %). These data confirm that gastric aspirate is no longer useful for the diagnosis of respiratory tuberculosis contrary to stool specimen.
Literature
1.
go back to reference World Health Organization (WHO) (2012) Global tuberculosis control: WHO report 2011. WHO, Geneva World Health Organization (WHO) (2012) Global tuberculosis control: WHO report 2011. WHO, Geneva
2.
go back to reference McNerney R, Maeurer M, Abubakar I, Marais B, McHugh TD, Ford N, Weyer K, Lawn S, Grobusch MP, Memish Z, Squire SB, Pantaleo G, Chakaya J, Casenghi M, Migliori GB, Mwaba P, Zijenah L, Hoelscher M, Cox H, Swaminathan S, Kim PS, Schito M, Harari A, Bates M, Schwank S, O’Grady J, Pletschette M, Ditui L, Atun R, Zumla A (2012) Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities. J Infect Dis 205(Suppl 2):S147–S158PubMedCrossRef McNerney R, Maeurer M, Abubakar I, Marais B, McHugh TD, Ford N, Weyer K, Lawn S, Grobusch MP, Memish Z, Squire SB, Pantaleo G, Chakaya J, Casenghi M, Migliori GB, Mwaba P, Zijenah L, Hoelscher M, Cox H, Swaminathan S, Kim PS, Schito M, Harari A, Bates M, Schwank S, O’Grady J, Pletschette M, Ditui L, Atun R, Zumla A (2012) Tuberculosis diagnostics and biomarkers: needs, challenges, recent advances, and opportunities. J Infect Dis 205(Suppl 2):S147–S158PubMedCrossRef
3.
go back to reference Djelouadji Z, Arnold C, Gharbia S, Raoult D, Drancourt M (2008) Multispacer sequence typing for Mycobacterium tuberculosis genotyping. PLoS One 3:e2433PubMedCrossRef Djelouadji Z, Arnold C, Gharbia S, Raoult D, Drancourt M (2008) Multispacer sequence typing for Mycobacterium tuberculosis genotyping. PLoS One 3:e2433PubMedCrossRef
4.
go back to reference World Health Organization (WHO) (2010) Multidrug and extensively drug-resistant TB (M/XDR-TB): 2010 global report on surveillance and response. WHO, Geneva World Health Organization (WHO) (2010) Multidrug and extensively drug-resistant TB (M/XDR-TB): 2010 global report on surveillance and response. WHO, Geneva
5.
go back to reference Pomputius WF 3rd, Rost J, Dennehy PH, Carter EJ (1997) Standardization of gastric aspirate technique improves yield in the diagnosis of tuberculosis in children. Pediatr Infect Dis J 16:222–226PubMedCrossRef Pomputius WF 3rd, Rost J, Dennehy PH, Carter EJ (1997) Standardization of gastric aspirate technique improves yield in the diagnosis of tuberculosis in children. Pediatr Infect Dis J 16:222–226PubMedCrossRef
6.
go back to reference Procop GW, Roberts GD (2011) Laboratory diagnosis and susceptibility testing. In: Schlossberg D (ed) Tuberculosis and nontuberculous mycobacterial infections, 6th edn. ASM Press, Washington DC, pp 66–74 Procop GW, Roberts GD (2011) Laboratory diagnosis and susceptibility testing. In: Schlossberg D (ed) Tuberculosis and nontuberculous mycobacterial infections, 6th edn. ASM Press, Washington DC, pp 66–74
7.
go back to reference Chierakul N, Anantasetagoon T, Chaiprasert A, Tingtoy N (2003) Diagnostic value of gastric aspirate smear and polymerase chain reaction in smear-negative pulmonary tuberculosis. Respirology 8:492–496PubMedCrossRef Chierakul N, Anantasetagoon T, Chaiprasert A, Tingtoy N (2003) Diagnostic value of gastric aspirate smear and polymerase chain reaction in smear-negative pulmonary tuberculosis. Respirology 8:492–496PubMedCrossRef
8.
go back to reference El Khéchine A, Henry M, Raoult D, Drancourt M (2009) Detection of Mycobacterium tuberculosis complex organisms in the stools of patients with pulmonary tuberculosis. Microbiology 155:2384–2389PubMedCrossRef El Khéchine A, Henry M, Raoult D, Drancourt M (2009) Detection of Mycobacterium tuberculosis complex organisms in the stools of patients with pulmonary tuberculosis. Microbiology 155:2384–2389PubMedCrossRef
9.
go back to reference El Khéchine A, Couderc C, Flaudrops C, Raoult D, Drancourt M (2011) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice. PLoS One 6:e24720PubMedCrossRef El Khéchine A, Couderc C, Flaudrops C, Raoult D, Drancourt M (2011) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice. PLoS One 6:e24720PubMedCrossRef
10.
go back to reference Donald PR, Schaaf HS, Gie RP, Beyers N, Sirgel FA, Venter A (1996) Stool microscopy and culture to assist the diagnosis of pulmonary tuberculosis in childhood. J Trop Pediatr 42:311–312PubMedCrossRef Donald PR, Schaaf HS, Gie RP, Beyers N, Sirgel FA, Venter A (1996) Stool microscopy and culture to assist the diagnosis of pulmonary tuberculosis in childhood. J Trop Pediatr 42:311–312PubMedCrossRef
11.
go back to reference Oberhelman RA, Soto-Castellares G, Gilman RH, Caviedes L, Castillo ME, Kolevic L, Del Pino T, Saito M, Salazar-Lindo E, Negron E, Montenegro S, Laguna-Torres VA, Moore DA, Evans CA (2010) Diagnostic approaches for paediatric tuberculosis by use of different specimen types, culture methods, and PCR: a prospective case–control study. Lancet Infect Dis 10:612–620PubMedCrossRef Oberhelman RA, Soto-Castellares G, Gilman RH, Caviedes L, Castillo ME, Kolevic L, Del Pino T, Saito M, Salazar-Lindo E, Negron E, Montenegro S, Laguna-Torres VA, Moore DA, Evans CA (2010) Diagnostic approaches for paediatric tuberculosis by use of different specimen types, culture methods, and PCR: a prospective case–control study. Lancet Infect Dis 10:612–620PubMedCrossRef
12.
go back to reference Oberhelman RA, Soto-Castellares G, Caviedes L, Castillo ME, Kissinger P, Moore DA, Evans C, Gilman RH (2006) Improved recovery of Mycobacterium tuberculosis from children using the microscopic observation drug susceptibility method. Pediatrics 118:e100–e106PubMedCrossRef Oberhelman RA, Soto-Castellares G, Caviedes L, Castillo ME, Kissinger P, Moore DA, Evans C, Gilman RH (2006) Improved recovery of Mycobacterium tuberculosis from children using the microscopic observation drug susceptibility method. Pediatrics 118:e100–e106PubMedCrossRef
13.
go back to reference Best M, Sattar SA, Springthorpe VS, Kennedy ME (1990) Efficacies of selected disinfectants against Mycobacterium tuberculosis. J Clin Microbiol 28:2234–2239PubMed Best M, Sattar SA, Springthorpe VS, Kennedy ME (1990) Efficacies of selected disinfectants against Mycobacterium tuberculosis. J Clin Microbiol 28:2234–2239PubMed
14.
go back to reference Rikimaru T, Kondo M, Kondo S, Oizumi K (2000) Efficacy of common antiseptics against mycobacteria. Int J Tuberc Lung Dis 4:570–576PubMed Rikimaru T, Kondo M, Kondo S, Oizumi K (2000) Efficacy of common antiseptics against mycobacteria. Int J Tuberc Lung Dis 4:570–576PubMed
15.
go back to reference Peres PJ, Gevaudan MJ, Gulian C, de Micco P (1988) Une méthode de traitement des produits pathologiques en vue de l’isolement des mycobactéries. Revue Fr Lab 173:67–74 Peres PJ, Gevaudan MJ, Gulian C, de Micco P (1988) Une méthode de traitement des produits pathologiques en vue de l’isolement des mycobactéries. Revue Fr Lab 173:67–74
16.
go back to reference Ferroni A, Vu-Thien H, Lanotte P, Le Bourgeois M, Sermet-Gaudelus I, Fauroux B, Marchand S, Varaigne F, Berche P, Gaillard JL, Offredo C (2006) Value of the chlorhexidine decontamination method for recovery of nontuberculous mycobacteria from sputum samples of patients with cystic fibrosis. J Clin Microbiol 44:2237–2239PubMedCrossRef Ferroni A, Vu-Thien H, Lanotte P, Le Bourgeois M, Sermet-Gaudelus I, Fauroux B, Marchand S, Varaigne F, Berche P, Gaillard JL, Offredo C (2006) Value of the chlorhexidine decontamination method for recovery of nontuberculous mycobacteria from sputum samples of patients with cystic fibrosis. J Clin Microbiol 44:2237–2239PubMedCrossRef
Metadata
Title
Gastric aspiration is not necessary for the diagnosis of pulmonary tuberculosis
Authors
P.-E. Bonnave
D. Raoult
M. Drancourt
Publication date
01-04-2013
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 4/2013
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-012-1776-6

Other articles of this Issue 4/2013

European Journal of Clinical Microbiology & Infectious Diseases 4/2013 Go to the issue
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.