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Published in: European Journal of Clinical Microbiology & Infectious Diseases 8/2012

01-08-2012 | Review

Next-generation and whole-genome sequencing in the diagnostic clinical microbiology laboratory

Authors: W. M. Dunne Jr, L. F. Westblade, B. Ford

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 8/2012

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Abstract

The identification and/or prediction of the antimicrobial resistance of microorganisms in clinical materials solely by molecular means in the diagnostic microbiology laboratory is not novel. However, the ability to sequence multitudes of bacterial genomes and deliver and interpret the resultant sequence information in near “real-time” is the basis of next-generation sequencing (NGS) technologies. There have been numerous applications and successes of NGS applications in the clinical and public health domain. However, none have, as yet, delivered perhaps the most sought after application, i.e., the generation of microbial sequence data for “real-time” patient management. In this review, we discuss the use of NGS and whole-genome sequencing (WGS) of microorganisms as a logical next step for the routine diagnosis of infection and the prediction of antimicrobial susceptibility in the clinical microbiology laboratory.
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Metadata
Title
Next-generation and whole-genome sequencing in the diagnostic clinical microbiology laboratory
Authors
W. M. Dunne Jr
L. F. Westblade
B. Ford
Publication date
01-08-2012
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 8/2012
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-012-1641-7

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