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Published in: European Journal of Clinical Microbiology & Infectious Diseases 1/2010

Open Access 01-01-2010 | Brief Report

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Authors: W. J. B. van Wamel, S. Hansenová Maňásková, A. C. Fluit, H. Verbrugh, A. J. de Neeling, E. van Duijkeren, A. van Belkum

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 1/2010

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Abstract

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.
Literature
1.
go back to reference van Loo IH, Diederen BM, Savelkoul PH, Woudenberg JH, Roosendaal R, van Belkum A et al (2007) Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg Infect Dis 13(11):1753–1755PubMed van Loo IH, Diederen BM, Savelkoul PH, Woudenberg JH, Roosendaal R, van Belkum A et al (2007) Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg Infect Dis 13(11):1753–1755PubMed
2.
go back to reference Welinder-Olsson C, Floren-Johansson K, Larsson L, Oberg S, Karlsson L, Ahren C (2008) Infection with Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus t034. Emerg Infect Dis 14(8):1271–1272CrossRefPubMed Welinder-Olsson C, Floren-Johansson K, Larsson L, Oberg S, Karlsson L, Ahren C (2008) Infection with Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus t034. Emerg Infect Dis 14(8):1271–1272CrossRefPubMed
3.
go back to reference Wulf MW, Markestein A, van der Linden FT, Voss A, Klaassen C, Verduin CM (2008) First outbreak of methicillin-resistant Staphylococcus aureus ST398 in a Dutch hospital, June 2007. Euro Surveill 28(9):13 Wulf MW, Markestein A, van der Linden FT, Voss A, Klaassen C, Verduin CM (2008) First outbreak of methicillin-resistant Staphylococcus aureus ST398 in a Dutch hospital, June 2007. Euro Surveill 28(9):13
4.
go back to reference Voss A, Loeffen F, Bakker J, Klaassen C, Wulf M (2005) Methicillin-resistant Staphylococcus aureus in pig farming. Emerg Infect Dis 11(12):1965–1966PubMed Voss A, Loeffen F, Bakker J, Klaassen C, Wulf M (2005) Methicillin-resistant Staphylococcus aureus in pig farming. Emerg Infect Dis 11(12):1965–1966PubMed
5.
go back to reference Yu F, Chen Z, Liu C, Zhang X, Lin X, Chi S et al (2008) Prevalence of Staphylococcus aureus carrying Panton-Valentine leukocidin genes among isolates from hospitalised patients in China. Clin Microbiol Infect 14(4):381–384CrossRefPubMed Yu F, Chen Z, Liu C, Zhang X, Lin X, Chi S et al (2008) Prevalence of Staphylococcus aureus carrying Panton-Valentine leukocidin genes among isolates from hospitalised patients in China. Clin Microbiol Infect 14(4):381–384CrossRefPubMed
6.
go back to reference van Belkum A, Melles DC, Peeters JK, van Leeuwen WB, van Duijkeren E, Huijsdens XW et al (2008) Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans. Emerg Infect Dis 14(3):479–483CrossRefPubMed van Belkum A, Melles DC, Peeters JK, van Leeuwen WB, van Duijkeren E, Huijsdens XW et al (2008) Methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398 in pigs and humans. Emerg Infect Dis 14(3):479–483CrossRefPubMed
7.
go back to reference Ip M, Yung RW, Ng TK, Luk WK, Tse C, Hung P et al (2005) Contemporary methicillin-resistant Staphylococcus aureus clones in Hong Kong. J Clin Microbiol 43(10):5069–5073CrossRefPubMed Ip M, Yung RW, Ng TK, Luk WK, Tse C, Hung P et al (2005) Contemporary methicillin-resistant Staphylococcus aureus clones in Hong Kong. J Clin Microbiol 43(10):5069–5073CrossRefPubMed
8.
go back to reference Boye K, Bartels MD, Andersen IS, Moller JA, Westh H (2007) A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V. Clin Microbiol Infect 13(7):725–727CrossRefPubMed Boye K, Bartels MD, Andersen IS, Moller JA, Westh H (2007) A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V. Clin Microbiol Infect 13(7):725–727CrossRefPubMed
9.
go back to reference Melles DC, Tenover FC, Kuehnert MJ, Witsenboer H, Peeters JK, Verbrugh HA et al (2008) Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals. J Clin Microbiol 46(1):235–241CrossRefPubMed Melles DC, Tenover FC, Kuehnert MJ, Witsenboer H, Peeters JK, Verbrugh HA et al (2008) Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals. J Clin Microbiol 46(1):235–241CrossRefPubMed
10.
go back to reference Lebon A, Labout JA, Verbrugh HA, Jaddoe VW, Hofman A, van Wamel W et al (2008) Dynamics and determinants of Staphylococcus aureus carriage in infancy: the Generation R Study. J Clin Microbiol 46(10):3517–3521CrossRefPubMed Lebon A, Labout JA, Verbrugh HA, Jaddoe VW, Hofman A, van Wamel W et al (2008) Dynamics and determinants of Staphylococcus aureus carriage in infancy: the Generation R Study. J Clin Microbiol 46(10):3517–3521CrossRefPubMed
11.
go back to reference Schijffelen MJ, Boel CHE, van Strijp JAG, Fluit AC (2009) Whole genome analysis of a pig-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis (in press) Schijffelen MJ, Boel CHE, van Strijp JAG, Fluit AC (2009) Whole genome analysis of a pig-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis (in press)
12.
go back to reference Herron-Olson L, Fitzgerald JR, Musser JM, Kapur V (2007) Molecular correlates of host specialization in Staphylococcus aureus. PLoS ONE 2(10):e1120CrossRefPubMed Herron-Olson L, Fitzgerald JR, Musser JM, Kapur V (2007) Molecular correlates of host specialization in Staphylococcus aureus. PLoS ONE 2(10):e1120CrossRefPubMed
Metadata
Title
Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398
Authors
W. J. B. van Wamel
S. Hansenová Maňásková
A. C. Fluit
H. Verbrugh
A. J. de Neeling
E. van Duijkeren
A. van Belkum
Publication date
01-01-2010
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 1/2010
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-009-0816-3

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