Published in:
Open Access
01-01-2010 | Brief Report
Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398
Authors:
W. J. B. van Wamel, S. Hansenová Maňásková, A. C. Fluit, H. Verbrugh, A. J. de Neeling, E. van Duijkeren, A. van Belkum
Published in:
European Journal of Clinical Microbiology & Infectious Diseases
|
Issue 1/2010
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Abstract
Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.