Top

European Journal of Clinical Microbiology & Infectious Diseases

Published in:

01-03-2009 | Concise Article

Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study

Authors: E. Biagi, B. Vitali, C. Pugliese, M. Candela, G. G. G. Donders, P. Brigidi

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 3/2009

Abstract

The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time.

Fredricks DN, Fiedler TL, Marrazzo JM (2005) Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 353:1899–1911 doi:10.1056/NEJMoa043802 PubMedCrossRef

Sobel JD, Chaim W (1996) Vaginal microbiology of women with acute recurrent vulvovaginal candidiasis. J Clin Microbiol 34:2497–2499PubMed

Rönnqvist PD, Forsgren-Brusk UB, Grahn-Håkansson EE (2006) Lactobacilli in the female genital tract in relation to other genital microbes and vaginal pH. Acta Obstet Gynecol Scand 85:726–735PubMedCrossRef

Hyman RW, Fukushima M, Diamond L, Kumm J, Giudice LC, Davis RW (2005) Microbes on the human vaginal epithelium. Proc Natl Acad Sci USA 102:7952–7957 doi:10.1073/pnas.0503236102 PubMedCrossRef

Eschenbach DA (1993) History and review of bacterial vaginosis. Am J Obstet Gynecol 169:441–445PubMed

Thies FL, König W, König B (2007) Rapid characterization of the normal and disturbed vaginal microbiota by application of 16S rRNA gene terminal RFLP fingerprinting. J Med Microbiol 56:755–761 doi:10.1099/jmm.0.46562-0 PubMedCrossRef

Zdolsek B, Hellberg D, Fröman G, Nilsson S, Mårdh PA (1995) Vaginal microbiological flora and sexually transmitted diseases in women with recurrent or current vulvovaginal candidiasis. Infection 23:81–84 doi:10.1007/BF01833870 PubMedCrossRef

Gibbs RS (2007) Asymptomatic bacterial vaginosis: is it time to treat? Am J Obstet Gynecol 196:495–496 doi:10.1016/j.ajog.2007.04.001 PubMedCrossRef

Zariffard MR, Saifuddin M, Sha BE, Spear GT (2002) Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis. FEMS Immunol Med Microbiol 34:277–281 doi:10.1111/j.1574-695X.2002.tb00634.x PubMedCrossRef

10.

Matsuki T, Watanabe K, Fujimoto J, Takada T, Tanaka R (2004) Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl Environ Microbiol 70:7220–7228 doi:10.1128/AEM.70.12.7220-7228.2004 PubMedCrossRef

11.

Matsuki T, Watanabe K, Fujimoto J, Miyamoto Y, Takada T, Matsumoto K, Oyaizu H, Tanaka R (2002) Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol 68:5445–5451PubMedCrossRef

12.

Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97:1166–1177 doi:10.1111/j.1365-2672.2004.02409.x PubMedCrossRef

13.

Heilig HGHJ, Zoetendal EG, Vaughan EE, Marteau P, Akkermans ADL, de Vos WM (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68:114–123 doi:10.1128/AEM.68.1.114-123.2002 PubMedCrossRef

14.

Sha BE, Chen HY, Wang QJ, Zariffard MR, Cohen MH, Spear GT (2005) Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. J Clin Microbiol 43:4607–4612 doi:10.1128/JCM.43.9.4607-4612.2005 PubMedCrossRef

15.

Vitali B, Pugliese C, Biagi E, Candela M, Turroni S, Bellen G, Donders GG, Brigidi P (2007) Dynamics of vaginal bacterial communities in women developing bacterial vaginosis, candidiasis, or no infection, analyzed by PCR-denaturing gradient gel electrophoresis and real-time PCR. Appl Environ Microbiol 73:5731–5741 doi:10.1128/AEM.01251-07 PubMedCrossRef

16.

De Backer E, Verhelst R, Verstraelen H, Alqumber MA, Burton JP, Tagg JR, Temmerman M, Vaneechoutte M (2007) Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners. BMC Microbiol 7:115 doi:10.1186/1471-2180-7-115 PubMedCrossRef

Title: Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study
Authors: E. Biagi
B. Vitali
C. Pugliese
M. Candela
G. G. G. Donders
P. Brigidi
Publication date: 01-03-2009
Publisher: Springer-Verlag
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 3/2009
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-008-0617-0

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2009

Severe adenovirus community-acquired pneumonia mimicking Legionella

Rapid detection of bla KPC -positive Klebsiella pneumoniae in a clinical setting

Idarubicin inhibits the growth of bacteria and yeasts in an automated blood culture system

A foodborne outbreak of a group A streptococcal infection in a Japanese university hospital

Are dental procedures an important risk factor for infective endocarditis? A case-crossover study

Inhaled aminoglycosides in cancer patients with ventilator-associated Gram-negative bacterial pneumonia: safety and feasibility in the era of escalating drug resistance

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page