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Open Access 01-12-2020 | Original Article

Analysis of resorbable mesh implants in short-term human muscular fascia cultures: a pilot study

Authors: V. Trapani, G. Bagni, M. Piccoli, I. Roli, F. Di Patti, A. Arcangeli

Published in: Hernia | Issue 6/2020

Abstract

Purpose

Alteration in fascial tissue collagen composition represents a key factor in hernia etiology and recurrence. Both resorbable and non-resorbable meshes for hernia repair are currently used in the surgical setting. However, no study has investigated so far the role of different implant materials on collagen deposition and tissue remodeling in human fascia. The aim of the present study was to develop a novel ex vivo model of human soft tissue repair mesh implant, and to test its suitability to investigate the effects of different materials on tissue remodeling and collagen composition.

Methods

Resorbable poly-4-hydroxybutyrate and non-resorbable polypropylene mesh implants were embedded in human abdominal fascia samples, mimicking common surgical procedures. Calcein-AM/Propidium Iodide vital staining was used to assess tissue vitality. Tissue morphology was evaluated using Mallory trichrome and hematoxylin and eosin staining. Collagen type I and III expression was determined through immunostaining semi-quantification by color deconvolution. All analyses were performed after 54 days of culture.

Results

The established ex vivo model showed good viability at 54 days of culture, confirming both culture method feasibility and implants biocompatibility. Both mesh implants induced a disorganization of collagen fibers pattern. A statistically significantly higher collagen I/III ratio was detected in fascial tissue samples cultured with resorbable implants compared to either non-resorbable implants or meshes-free controls.

Conclusion

We developed a novel ex vivo model and provided evidence that resorbable polyhydroxybutyrate meshes display better biomechanical properties suitable for proper restoration in surgical hernia repair.

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Title: Analysis of resorbable mesh implants in short-term human muscular fascia cultures: a pilot study
Authors: V. Trapani
G. Bagni
M. Piccoli
I. Roli
F. Di Patti
A. Arcangeli
Publication date: 01-12-2020
Publisher: Springer Paris
Published in: Hernia / Issue 6/2020
Print ISSN: 1265-4906
Electronic ISSN: 1248-9204
DOI: https://doi.org/10.1007/s10029-020-02271-x

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Analysis of resorbable mesh implants in short-term human muscular fascia cultures: a pilot study

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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