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Medical Molecular Morphology
Published in: Medical Molecular Morphology 2/2017

01-06-2017 | Original Paper

Enhancement of adherence of Helicobacter pylori to host cells by virus: possible mechanism of development of symptoms of gastric disease

Authors: Hong Wu, Takashi Nakano, Youichi Suzuki, Yukimasa Ooi, Kouichi Sano

Published in: Medical Molecular Morphology | Issue 2/2017

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Abstract

It remains unclear why gastric disease does not develop in all cases of Helicobacter pylori infection. In this study, we analyzed whether simian virus 5 (SV5) enhanced adherence of H. pylori to adenocarcinoma epithelial cells (AGS). H. pylori in AGS (harboring SV5) and SV5-infected Vero cells, and an agglutination of H. pylori mixed with SV5 were observed by light microscopy, scanning and transmission electron microscopies. The adherent rate of H. pylori to SV5-infected Vero cells and treated with an anti-SV5 antibody was determined. H. pylori adhered to the surface of AGS cells near SV5 particles, as shown by scanning and transmission electron microscopies. The adherence of H. pylori to SV5-infected Vero cells was significantly enhanced compared with that to Vero cells. In contrast, the adherence of H. pylori to Vero cells was decreased by treatment with the anti-SV5 antibody. Agglutination of H. pylori mixed with SV5 was observed by scanning and transmission electron microscopies. Agglutination did not occur when SV5 was treated with the anti-SV5 antibody before mixing. These findings demonstrated that SV5 enhanced the adherence of H. pylori to host cells, suggesting that a persistently infected virus may be a factor enhancing the pathogenicity of H. pylori in humans.
Literature
1.
Warren JR, Marshall B (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1:1273–1275
2.
Bohnen JM, Krajden S, Anderson JG, Kempston JD, Fuksa M, Karmali MA, Osborne A, Babida C (1986) Campylobacter pyloridis is associated with acid-peptic disease in Toronto. Can J Surg 29:442–444PubMed
3.
Wadstorm T (1995) An update on Helicobacter pylori. Curr Opin Gastroenterol 11:69–75CrossRef
4.
Kurosawa M, Kikuchi S, Inaba Y, Ishibashi T, Kobayashi F (2000) Helicobacter pylori infection among Japanese children. J Gastroenterol Hepatol 15:1382–1385CrossRefPubMed
5.
Cantorna MT, Balish E (1990) Inability of human clinical strains of Helicobacter pylori to colonize the alimentary tract of germfree rodents. Can J Microbiol 36:237–241CrossRefPubMed
6.
Varro A, Noble PJ, Pritchard DM, Kennedy S, Hart CA, Dimaline R, Dockray GJ (2004) Helicobacter pylori induces plasminogen activator inhibitor 2 in gastric epithelial cells through nuclear factor-κB and RhoA: implications for invasion and apoptosis. Cancer Res 64:1695–1702CrossRefPubMed
7.
Backert S, Moese S, Selbach M, Brinkmann V, Meyer TF (2001) Phosphorylation of tyrosine 972 of the Helicobacter pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells. Mol Microbiol 42:631–644CrossRefPubMed
8.
Segal ED, Cha J, Lo J, Falkow S, Tompkins LS (1999) Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc Natl Acad Sci USA 96:14559–14564CrossRefPubMedCentralPubMed
9.
Tsutsumi R, Higashi H, Higuchi M, Okada M, Hatakeyama M (2003) Attenuation of Helicobacter pylori CagA x SHP-2 signaling by interaction between CagA and C-terminal Src kinase. J Biol Chem 278:3664–3670CrossRefPubMed
10.
Iwakura K, Wu H, Nakano T, Daikoku E, Shimamoto C, Katsu K, Sano K (2007) Unique enhancement of multinuclear giant cell formation in AGS cell line infected with Helicobacter pylori. Bull OMC 53:1–9
11.
Young DF, Carlos TS, Hagmaier K, Fan L, Randall RE (2007) AGS and other tissue culture cells can unknowingly be persistently infected with PIV5; A virus that blocks interferon signalling by degrading STAT1. Virology 365:238–240CrossRefPubMed
12.
Ooi Y, Daikoku E, Wu H, Aoki H, Morita C, Nakano T, Kohno T, Takasaki T, Sano K (2011) Morphology and infectivity of virus that persistently caused infection in an AGS cell line. Med Mol Morphol 44:213–220CrossRefPubMed
13.
Yuan P, Thompson TB, Wurzburg BA, Paterson RG, Lamb RA, Jardetzky TS (2005) Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose. Structure 13:803–815CrossRefPubMed
14.
Shao L, Takeda H, Fukui T, Mabe K, Han J, Kawata S, Ootani K, Fukao A (2010) Genetic diversity of the Helicobacter pylori sialic acid-binding adhesin (sabA) gene. Biosci Trends 4:249–253PubMed
15.
Åberg A, Gideonsson P, Vallström A, Olofsson A, Öhman C, Rakhimova L, Borén T, Engstrand L, Brännström K, Arnqvist A (2014) A repetitive DNA element regulates expression of the Helicobacter pylori sialic acid binding adhesin by a rheostat-like mechanism. PLoS Pathog 10:e1004234CrossRefPubMedCentralPubMed
16.
Kohno T, Mohan S, Goto T, Morita C, Nakano T, Hong W, Sangco JC, Morimatsu S, Sano K (2002) A new improved method for the concentration of HIV-1 infective particles. J Virol Methods 106:167–173CrossRefPubMed
17.
Morimatsu S, Yanagida J, Imanishi A, Nakano T, Wu H, Fujioka Y, Morita C, Goto T, Fukuda Y, Shimoyama T, Yamaguchi N, Sano K (2000) Detection of spiral, large coccoid and small coccoid forms of Helicobacter pylori by a simple staining. Bull OMC 46:35–40
18.
Barranco SC, Townsend CM Jr, Casartelli C, Macik BG, Burger NL, Boerwinkle WR, Gourley WK (1983) Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res 43:1703–1709PubMed
19.
Gowda KL, Marie MA, John J, Pradeep CS, Dabwan KH, Sangeetha G (2014) Cytopathic effects of toxogenic strains of Helicobacter pylori on different cell lines. Indian J Pathol Microbiol 57:187–190CrossRefPubMed
20.
Camargo MC, Kim WH, Chiaravalli AM, Kim KM, Corvalan AH, Matsuo K, Yu J, Sung JJ, Herrera-Goepfert R, Meneses-Gonzalez F, Kijima Y, Natsugoe S, Liao LM, Lissowska J, Kim S, Hu N, Gonzalez CA, Yatabe Y, Koriyama C, Hewitt SM, Akiba S, Gulley ML, Taylor PR, Rabkin CS (2014) Improved survival of gastric cancer with tumour Epstein–Barr virus positivity: an international pooled analysis. Gut 63:236–243CrossRefPubMed
21.
Figueiredo C, Costa S, Karameris A, Machado JC (2015) Pathogenesis of gastric cancer. Helicobacter 20:30–35CrossRefPubMed
22.
Camargo MC, Kim KM, Matsuo K, Torres J, Liao LM, Morgan DR, Michel A, Waterboer T, Zabaleta J, Dominguez RL, Yatabe Y, Kim S, Rocha-Guevara ER, Lissowska J, Pawlita M, Rabkin CS (2016) Anti-Helicobacter pylori antibody profiles in Epstein–Barr virus (EBV)-positive and EBV-negative gastric cancer. Helicobacter 21:153–157CrossRef
23.
Okamoto S, Kawabata S, Nakagawa I, Okuno Y, Goto T, Sano K, Hamada S (2003) Influenza A virus-infected hosts boost an invasive type of Streptococcus pyogenes infection in mice. J Virol 77:4104–4112CrossRefPubMedCentralPubMed
Metadata
Title
Enhancement of adherence of Helicobacter pylori to host cells by virus: possible mechanism of development of symptoms of gastric disease
Authors
Hong Wu
Takashi Nakano
Youichi Suzuki
Yukimasa Ooi
Kouichi Sano
Publication date
01-06-2017
Publisher
Springer Japan
Published in
Medical Molecular Morphology / Issue 2/2017
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI
https://doi.org/10.1007/s00795-017-0153-z

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